Enabling breeding for brown rot (<i>Monilinia</i> spp.) resistance in Clemson University peach breeding program

Fu, Wanfang; Burrell, R. C.; Schnabel, Guido; Gašić, Ksenija

doi:10.17660/actahortic.2021.1304.10

Cited by 6 publications

(12 citation statements)

References 15 publications

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“…The genotypes located in the lower susceptibility group with lower values for both LD and LA were Conserva 947, 'Bolinha', Conserva 1600, Conserva 672, and 'Maciel' (Table 1). The cultivar Bolinha is known for having the lowest LD in several other studies performed in nonwounded fruits (Feliciano et al, 1987;Santos et al, 2012;Scariotto et al, 2015;Fu et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…For the inoculation on wounded fruits (wound penetration of 1 mm into the fruits with the syringe tip), it was used an inoculation syringe of 100 μL coupled to a repeating dispenser 50x (Hamilton ® ). In both, wounded and non-wounded fruits, 10 μL suspention of M. fructicola (2.5 × 10 4 conidia mL -1 ) with Tween-80 ® (0.1 g L −1 ) was inoculated in the equatorial region of each fruit (Crisosto, Gradziel, Ogundiwin, Bostock, & Michailides, 2009;Martínez-García et al, 2013;Scariotto et al, 2015;Fu et al, 2018;Obi et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…However, the use of this strategy is limited in commercial orchards because, although there are significant differences in susceptibility among available genotypes, commercial cultivars that are resistant to brown rot are not yet available (Santos & Ueno, 2014). The Brazilian peach cultivar Bolinhapresents a lower susceptibility than other cultivars already evaluated (Feliciano, Feliciano, & Ogawa, 1987, Santos, Raseira, & Zanandrea, 2012Fu, Burrell, Linge, Schnabel, & Gasic, 2018). However, poor fruit quality and productivity discouraged its cultivation.…”

Section: Introductionmentioning

confidence: 97%

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Peach and nectarine susceptibility to brown rot and protocol optimization to evaluate Monilinia fructicola sporulation

Dini

Raseira²,

Scariotto³

et al. 2022

Acta Sci. Agron.

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The fungus Monilinia fructicola, which causes brown rot in fruits, is one of the main peach pathogens. The emergence of fungicide-resistant fungus isolates, as well as the attempt to reduce sprays, favors adoption of other control strategies. Among them, one of the most important is genetic resistance. This study was carried out aiming to evaluate the susceptibility of 16 peach and 4 nectarine genotypes to brown rot, as well as to evaluate how well the sporulation area and diameter correlate with number of spores in the lesions. Both wounded and non-wounded fruits were inoculated with 10 μL of M. fructicola suspension. Wounded fruits from all genotypes (nectarines and peaches) showed susceptibility to M. fructicola, from 92 to 100% of incidence. The disease incidence was between 18 and 100% when non-wounded fruits were inoculated. High variability was detected for the fungus sporulation, in both wounded and non-wounded fruits, with ranges between 16 to 96% and 0 to 94%, respectively. The fungus sporulation was variable among the genotypes (between 0.1 to 96.0 conidia per mm2) and it is positively correlated with the diameter and area of sporulation. The genotypes Conserva 947, Conserva 1662, Conserva 672, Conserva 1600, and 'Bolinha', are the ones with less susceptible to brown rot.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Peach and nectarine susceptibility to brown rot and protocol optimization to evaluate Monilinia fructicola sporulation

Dini

Raseira²,

Scariotto³

et al. 2022

Acta Sci. Agron.

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“…Different authors have carried out phenotypic studies in peach evaluating brown rot tolerance (reviewed in Obi et al 2018). However, large-scale phenotyping is complex (Obi et al 2019) and the quantitative control of the trait (Martínez-García et al 2013a;Casals et al 2015;Fu et al 2018;Lloret et al 2018) adds certain difficulty. Phenotyping is based on inoculation under controlled conditions and the main parameters recorded to asses tolerance-susceptibility are lesion diameter, brown rot incidence, and disease and colonization severity (Obi et al 2018).…”

Section: Biotic Stress Tolerancementioning

confidence: 99%

“…8.6.3.2). The Brazilian peach cultivar Bolinha has been used traditionally in different breeding programs to introduce resistance to brown rot (Feliciano et al 1987;Fu et al 2018).…”

Section: Biotic Stress Tolerancementioning

confidence: 99%

Breeding Climate-Resilient Bananas

Brown

Carpentier

Swennen

2020

Genomic Designing of Climate-Smart Fruit Crops

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Genome-wide association analysis of Monilinia fructicola lesion in a collection of Spanish peach landraces

Martínez-García,

Mas-Gómez,

Prudencio

et al. 2023

Front. Plant Sci.

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Brown rot, caused by the Monilinia spp., is the disease that causes the greatest losses in stone fruit worldwide. Currently, M. fructicola has become the dominant species in the main peach production area in Spain. The fruit cuticle is the first barrier of protection against external aggressions and may have a key role in the susceptibility to brown rot. However, information on the role of skin fruit on the resistance to brown rot in peach is scarce. Previous genetic analyses in peach have demonstrated that brown rot resistance is a complex and quantitative trait in which different fruit parts and resistance mechanisms are involved. To search for genomic areas involved in the control of the cultivar susceptibility to brown rot and to elucidate the role of fruit skin against this infection, we have studied, for two consecutive seasons (2019 and 2020), the fruit susceptibility to M. fructicola, together with fruit cuticle thickness (CT) and density (CD), in a collection of 80 Spanish and 5 foreign peach cultivars from the National Peach Collection at CITA (Zaragoza, Spain). Brown rot incidence, lesion diameter, and severity index were calculated after 5 days of inoculation on non-wounded fruit. The peach collection has also been genotyped using the new peach SNP chip (9 + 9K). Genotypic and phenotypic data have been used to perform a genome-wide association analysis (GWAS). Phenotyping has shown a wide variability on the brown rot susceptibility within the Spanish germplasm as well as on CD and CT. The GWAS results have identified several significant SNPs associated with disease severity index (DSI), CD, and CT, five of which were considered as reliable SNP-trait associations. A wide protein network analysis, using 127 genes within the regions of the reliable SNPs and previously identified candidate genes (169) associated with Monilinia spp. resistance, highlighted several genes involved in classical hypersensitive response (HR), genes related to wax layers as ceramidases and lignin precursors catalyzers, and a possible role of autophagy during brown rot infection. This work adds relevant information on the complexity resistance mechanisms to brown rot infection in peach fruits and the genetics behind them.

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Enabling breeding for brown rot (Monilinia spp.) resistance in Clemson University peach breeding program

Cited by 6 publications

References 15 publications

Peach and nectarine susceptibility to brown rot and protocol optimization to evaluate Monilinia fructicola sporulation

Peach and nectarine susceptibility to brown rot and protocol optimization to evaluate Monilinia fructicola sporulation

Breeding Climate-Resilient Bananas

Genome-wide association analysis of Monilinia fructicola lesion in a collection of Spanish peach landraces

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