Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress

Skórkowska-Telichowska, Katarzyna; Hasiewicz-Derkacz, Karolina; Gębarowski, Tomasz; Kulma, Anna; Moreira, Helena; Kostyń, Kamil; Gębczak, Katarzyna; Szyjka, Anna; Wojtasik, Wioleta; Gąsiorowski, Kazimierz

doi:10.1155/2016/7510759

Cited by 13 publications

(10 citation statements)

References 50 publications

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“…The effect was accompanied by changes in gene expression, particularly those coding for histone, acetylase, deacetylase, proapoptotic, pro‐inflammatory, and anti‐inflammatory genes. This beneficial effect correlated with the amount of squalene present in emulsions . Likewise, dietary squalene downregulated cyclooxygenase‐2 and inducible nitric oxide synthase, thereby improving the oxidative events, in an acute colitis model in mice.…”

Section: Squalene and The Immune Systemmentioning

confidence: 86%

Current Insights into the Biological Action of Squalene

Lou-Bonafonte

Martínez‐Beamonte

Sanclemente

et al. 2018

Molecular Nutrition Food Res

115

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Squalene is a triterpenic compound found in a large number of plants and other sources with a long tradition of research since it was first reported in 1926. Herein a systematic review of studies concerning squalene published in the last 8 years is presented. These studies have provided further support for its antioxidant, anti-inflammatory, and anti-atherosclerotic properties in vivo and in vitro. Moreover, an antineoplastic effect in nutrigenetic-type treatments, which depends on the failing metabolic pathway of tumors, has also been reported. The bioavailability of squalene in cell cultures, animal models, and in humans has been well established, and further progress has been made in regard to the intracellular transport of this lipophilic molecule. Squalene accumulates in the liver and decreases hepatic cholesterol and triglycerides, with these actions being exerted via a complex network of changes in gene expression at both transcriptional and post-transcriptional levels. Its presence in different biological fluids has also been studied. The combination of squalene with other bioactive compounds has been shown to enhance its pleiotropic properties and might lead to the formulation of functional foods and nutraceuticals to control oxidative stress and, therefore, numerous age-related diseases in human and veterinary medicine.

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Section: Squalene and The Immune Systemmentioning

confidence: 86%

Current Insights into the Biological Action of Squalene

Lou-Bonafonte

Martínez‐Beamonte

Sanclemente

et al. 2018

Molecular Nutrition Food Res

115

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“…A collective assessment of the p53-induced antitumor activity of the tested compounds for all performed assays was carried out using multiple-criteria decision analysis (MCDA). The MCDA was performed according to literature procedures [11][12][13]. The assay results were calculated according to the formula: b = (exp-obs) 2 /exp (exp-expected value; obs-observed value).…”

Section: Discussionmentioning

confidence: 99%

Effect of new olivacine derivatives on p53 protein level

et al. 2020

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Background The p53 protein is a transcription factor for many genes, including genes involved in inhibiting cell proliferation and inducing apoptosis in genotoxically damaged and tumor-transformed cells. In more than 55% of cases of human cancers, loss of the essential function of p53 protein is found. In numerous reports, it has been shown that small molecules (chemical compounds) can restore the suppressor function of the mutant p53 protein in tumor cells. The aim of this study was to evaluate the potential anticancer activity of three newly synthesized olivacine derivatives. Methods The study was performed using two cell lines-CCRF/CEM (containing the mutant p53 protein) and A549 (containing a non-mutant, wild-type p53 protein). The cells were incubated with olivacine derivatives for 18 h and then assays were carried out: measurement of the amount of p53 and p21 proteins, detection of apoptosis, cell cycle analysis, and rhodamine 123 accumulation assay (evaluation of P-glycoprotein inhibition). Multiple-criteria decision analysis was used to compare the anticancer activity of the tested compounds. Results Each tested compound caused the reconstitution of suppressor activity of the p53 protein in cells with the mutant protein. In addition, one of the compounds showed significant antitumor activity in both wild-type and mutant cells. For all compounds, a stronger effect on the level of the p53 protein was observed than for the reference compound-ellipticine. Conclusions The observed effects of the tested new olivacine derivatives (pyridocarbazoles) suggest that they are good candidates for new anticancer drugs.

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“…For in vitro studies, the raspberry oil emulsions were formulated in accordance with a protocol developed in a study by Skorkowska-Telichowska et al [ 26 ]. One gram of soybean lecithin, 0.5 ml of Tween 80, and 2.5 ml of oil were mixed gradually and completed with a 5 ml of an aqueous phase containing glycerol 25% v/v.…”

Section: Methodsmentioning

confidence: 99%

“…Collision energy was set at 0, 20, 20-30, 30, and 30-50 kV. Data were processed using MassLynx 2.0 (Waters, Milford, CT, USA [26]. One gram of soybean lecithin, 0.5 ml of Tween 80, and 2.5 ml of oil were mixed gradually and completed with a 5 ml of an aqueous phase containing glycerol 25% v/v.…”

Section: Phenolic Compounds' Compositions and Contentmentioning

confidence: 99%

Bioactive Compounds of Raspberry Oil Emulsions Induced Oxidative Stress via Stimulating the Accumulation of Reactive Oxygen Species and NO in Cancer Cells

Grajzer

Wiatrak

Gębarowski

et al. 2021

Oxidative Medicine and Cellular Longevity

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There are growing interests in the complex combinations of natural compounds that may advance the therapy of cancer. Such combinations already exist in foods, and a good representative is seed oils. Two raspberry oils: cold pressed (ROCOP) and one extracted by supercritical CO2 (ROSCO2) were evaluated for their chemical characteristics and oil emulsions for cell suppression potential against colon adenocarcinoma (LoVo), doxorubicin-resistant colon adenocarcinoma (LoVo/DX), breast cancer (MCF7), doxorubicin-resistant breast cancer (MCF7/DX), and lung cancer (A549) cell lines. The cytotoxicity was also assessed on normal human dermal fibroblasts (NHDFs). With increasing concentration of raspberry oil emulsions (0.5–10%), increasing inhibition of cancer cell viability and proliferation in all of the lines was observed, with different degrees of potency between cancer types and oil tested. ROSCO2 strongly induced free radical production and DNA strand damage in LoVo and MCF7 cells especially doxorubicin-resistant lines. This suggests that ROSCO2 engages and effectively targets the vulnerabilities of the cancer cell. Generally, both ROSCO2 and ROCOP could be a nontoxic support in therapy of selected human cancers.

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Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress

Cited by 13 publications

References 50 publications

Current Insights into the Biological Action of Squalene

Current Insights into the Biological Action of Squalene

Effect of new olivacine derivatives on p53 protein level

Bioactive Compounds of Raspberry Oil Emulsions Induced Oxidative Stress via Stimulating the Accumulation of Reactive Oxygen Species and NO in Cancer Cells

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