Bioactive Compounds of Raspberry Oil Emulsions Induced Oxidative Stress via Stimulating the Accumulation of Reactive Oxygen Species and NO in Cancer Cells

Abstract

There are growing interests in the complex combinations of natural compounds that may advance the therapy of cancer. Such combinations already exist in foods, and a good representative is seed oils. Two raspberry oils: cold pressed (ROCOP) and one extracted by supercritical CO2 (ROSCO2) were evaluated for their chemical characteristics and oil emulsions for cell suppression potential against colon adenocarcinoma (LoVo), doxorubicin-resistant colon adenocarcinoma (LoVo/DX), breast cancer (MCF7), doxorubicin-resistant breast cancer (MCF7/DX), and lung cancer (A549) cell lines. The cytotoxicity was also assessed on normal human dermal fibroblasts (NHDFs). With increasing concentration of raspberry oil emulsions (0.5–10%), increasing inhibition of cancer cell viability and proliferation in all of the lines was observed, with different degrees of potency between cancer types and oil tested. ROSCO2 strongly induced free radical production and DNA strand damage in LoVo and MCF7 cells especially doxorubicin-resistant lines. This suggests that ROSCO2 engages and effectively targets the vulnerabilities of the cancer cell. Generally, both ROSCO2 and ROCOP could be a nontoxic support in therapy of selected human cancers.

Oils from Transgenic Flax Lines as Potential Chemopreventive Agents in Colorectal Cancer

“…Especially, the M line oil contained significantly more polyphenols. As we have shown in our previous studies, the subtle differences in composition of the minor bioactives in oils may result in diverse effectiveness in normal and cancer cells [ 24 , 25 ]. The observed inhibitory impact of B oil on neoplastic cell proliferation could be partially attributed to its marginally elevated stigmasterol concentration compared to the other examined oils.…”

“…Flaxseed oils were tested at final concentrations of 0.1 mg, 0.25 mg, 0.5 mg, 1.0 mg, and 2.5 mg per 1 mL of the medium at room temperature and in sterile conditions. The range of concentrations of the tested oils used is not toxic to cells and allows for chemopreventive effects in cancer cell model [ 25 ]. The MB oil emulsions were prepared by mixing 1:1 B and M oil ( v / v ).…”

“…As challenges like drug resistance encroach upon the efficacy of traditional chemotherapy, the scientific community is gravitating towards natural, cost-efficient agents that counteract CRC [ 20 , 21 , 22 , 23 ]. Seeds oils, including rose and raspberry seeds oils, have demonstrated chemopreventive effects in colon cancer cell models and hold promise as prophylactic agents or supplements to traditional chemotherapy [ 24 , 25 ].…”

A Review on Berry Seeds—A Special Emphasis on Their Chemical Content and Health-Promoting Properties

“…Recently, Grazjer et al have studied two raspberry oils (0.5–10%): cold-pressed and extracted by supercritical CO 2 . They have observed that both tested oils are good candidates for non-toxic support in the therapy of selected human cancers, including colon adenocarcinoma (LoVo), breast cancer (MCF7), and lung cancer (A549) cell lines [ 41 ].…”

Reorganization Reaction Characteristics between Different Volatile Content and Waste Pyrolysis

“…Oxygenic compounds [28][29][30] in biomass thermal electrolyte mainly contain carboxyl, base, and hydroxyl groups, and the reduction of oxygenic compounds is mainly due to the removal reaction of these three groups. 1), mainly the fracture of -COOH bond, mainly organic acids, including acetic acid, oleic acid, and palmitate, which will produce methane or ethylene; oleic acid and palmitate are usually saturated alkane.…”