Emulsion Template Method for the Fabrication of Gelatin-Based Scaffold with a Controllable Pore Structure

Long, Yuan; Li, Xinying; Ge, Liming; Jia, Xiaoqi; Lei, Jinfeng; Mu, Changdao; Li, Defu

doi:10.1021/acsami.8b17555

Cited by 57 publications

(42 citation statements)

References 70 publications

(109 reference statements)

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“…Due to the amphiphilic nature of gelatin-graft-PNIPAM as a continuous phase, they managed to incorporate an internal phase of up to 90% without the use of any surfactants. Recently Yuan et al (2019) reported the fabrication of gelatin PolyHIPEs with 92% porosity by two-step crosslinking and freeze-drying. Alginate, a polysaccharide derived from seaweed, is another biomaterial that can be used to fabricate PolyHIPE scaffolds (Barbetta et al, 2009;Zhou et al, 2013).…”

Section: Hydrophilic Polymers For the Fabrication Of O/w Polyhipesmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims to regenerate critical size defects, which cannot heal naturally, by using highly porous matrices called TE scaffolds made of biocompatible and biodegradable materials. There are various manufacturing techniques commonly used to fabricate TE scaffolds. However, in most cases, they do not provide materials with a highly interconnected pore design. Thus, emulsion templating is a promising and convenient route for the fabrication of matrices with up to 99% porosity and high interconnectivity. These matrices have been used for various application areas for decades. Although this polymer structuring technique is older than TE itself, the use of polymerised internal phase emulsions (PolyHIPEs) in TE is relatively new compared to other scaffold manufacturing techniques. It is likely because it requires a multidisciplinary background including materials science, chemistry and TE although producing emulsion templated scaffolds is practically simple. To date, a number of excellent reviews on emulsion templating have been published by the pioneers in this field in order to explain the chemistry behind this technique and potential areas of use of the emulsion templated structures. This particular review focusses on the key points of how emulsion templated scaffolds can be fabricated for different TE applications. Accordingly, we first explain the basics of emulsion templating and characteristics of PolyHIPE scaffolds. Then, we discuss the role of each ingredient in the emulsion and the impact of the compositional changes and process conditions on the characteristics of PolyHIPEs. Afterward, current fabrication methods of biocompatible PolyHIPE scaffolds and polymerisation routes are detailed, and the functionalisation strategies that can be used to improve the biological activity of PolyHIPE scaffolds are discussed. Finally, the applications of PolyHIPEs on soft and hard TE as well as in vitro models and drug delivery in the literature are summarised.

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Section: Hydrophilic Polymers For the Fabrication Of O/w Polyhipesmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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“…The particle size and morphology of the microspheres are affected by many factors in the emulsification process, such as oil−water ratio, stirring speed, and concentration of sodium alginate. 46 Besides, the particle size and morphology of the resulting microspheres are related to the adding way of cross- link agent too. Uniform particle size of microspheres makes drug-release process controllable and reproducible, which is beneficial to predict the release kinetics of drugs.…”

Section: Resultsmentioning

confidence: 99%

Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Mao

Zhang

et al. 2019

ACS Appl. Bio Mater.

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Bioactive peptide drugs are mostly delivered by parenteral administration, which brings great pain and risks to patients. Oral administration is an acceptable alternative form. However, peptide drugs are extremely sensitive to the strong acidic environment in the stomach after oral administration. They would be degraded by pepsin and trypsin in the gastrointestinal tract. Herein, we present microspheres for intestinal-targeted peptides drug delivery through oral administration. Sodium alginate was reacted with l-cysteine to bring it into thiol groups. Then sodium alginate-l-cysteine conjugates were mixed with native sodium alginate and emulsified by an improved method. Ca2+ was used to fix the emulsion to get the microspheres. Bovine serum albumin was used as the simulating drug to assess the feasibility of microspheres as intestinal delivery carriers. The results showed that the microspheres exhibited spherical properties and narrow size distribution. The drug-loading capacity of microspheres was not compromised after thiol-modification. It is interesting that the microspheres can maintain structural integrity and hold drugs in the strong acidic environment in the stomach. Conversely, the microspheres presented sustained intestinal-targeted drugs release ability as expected. Moreover, thiol-modification further improved the adherence ability of microspheres on the inner walls of the small intestine, which is good for enhancing drug permeability. In sum, the microspheres based on thiol-modified sodium alginate have promising applications as intestinal-targeted macromolecular drug carriers.

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“…The mouse osteoblasts (MC3T3-E1) and mouse myoblasts (C2C12) were used to evaluate the cytocompatibility of samples. The cytotoxicity of samples was evaluated by traditional CCK-8 methods. , The culture medium was high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (penicillin and streptomycin). The ultraviolet-sterilized samples were immersed in culture medium for 24 h at 37 °C to obtain the extraction liquid.…”

Section: Methodsmentioning

confidence: 99%

Controlling the Pore Structure of Collagen Sponge by Adjusting the Cross-Linking Degree for Construction of Heterogeneous Double-Layer Bone Barrier Membranes

Zhao

Xie

et al. 2020

ACS Appl. Bio Mater.

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Guided bone regeneration (GBR) has been regarded as a valuable way to effectively induce bone remodeling. The key factor of GBR is to place a barrier membrane between the soft tissue and bone defect, preventing the untimely intrusion of fibroblasts and permitting the prior settlement of internal osteoblasts. Notably, heterogeneous double-layer GBR membranes with a compact upper layer and a loose lower layer exhibit enhanced effectiveness in blocking fibroblasts and promoting the growth of osteoblasts. Herein, we present porous and interconnected collagen-based sponges with controllable pore size for the fabrication of absorbable GBR membranes with a heterogeneous double-layer structure. Dialdehyde carboxymethyl cellulose was used to fix collagen-based sponges. The pore size of the sponges can be well controlled by adjusting the cross-linking degree, which is decreased with an increase of cross-linking degree. The sponges show enhanced mechanical properties, inhibited swelling ability and biodegradation, good blood compatibility, and good cytocompatibility. The sponges are feasible to form a heterogeneous double-layer structure with a loose lower layer and a compact upper layer. Interestingly, the lower layer with the pore size of 200–300 μm can promote the adhesion, proliferation, and differentiation of osteoblasts MC3T3-E1 cells, while the upper layer with the pore size of 20–50 μm makes a great contribution to the growth of myoblast C2C12 cells. Overall, the collagen-based sponges have the potential to be used to fabricate heterogeneous double-layer bone barrier membranes for bone remodeling.

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Emulsion Template Method for the Fabrication of Gelatin-Based Scaffold with a Controllable Pore Structure

Cited by 57 publications

References 70 publications

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Development of Microspheres Based on Thiol-Modified Sodium Alginate for Intestinal-Targeted Drug Delivery

Controlling the Pore Structure of Collagen Sponge by Adjusting the Cross-Linking Degree for Construction of Heterogeneous Double-Layer Bone Barrier Membranes

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