Emulsifying Properties of Polysaccharide Conjugates Prepared from Chin-Brick Tea

Abstract

Chin-brick tea polysaccharide conjugates (TPC-C) were prepared to study their emulsion capabilities. Interfacial tension and the effects of some factors, such as storage time, metal ion concentrations (Na+, Ca2+), pH (2.0–8.0), and heat treatment (70–100 °C) on the emulsions stabilized by TPC-C were studied. The interfacial tension of TPC-C (10.88 mN/m) was lower than that of gum arabic (15.18 mN/m) at a concentration of 0.08%. As the TPC-C concentration increased from 0.1 to 3.0 wt %, the mean particle diameter (MPD) (d 32) of emulsions stabilized by TPC-C decreased from 1.88 to 0.16 μm. Furthermore, at a concentration of 0.5 wt % or higher, the MPD (d 32) of emulsions stabilized by TPC-C at 25 and 60 °C for 10 days was between 0.20 and 0.50 μm. In the tested pH conditions from 2.0 to 8.0, the MPD (d 32) of emulsions stabilized by 2.0 wt % TPC-C was less than 0.20 μm. At Na+ concentration conditions between 0.10 and 0.50 mol/L, the MPD (d 32) of emulsions was between 0.19 and 0.20 μm, and the zeta potential values varied from −34.10 to −32.60 mV. However, with an increasing Ca2+ concentration from 0.01 to 0.05 mol/L, the MPD (d 32) of emulsions was between 0.20 and 21.65 μm, and the zeta potential raised sharply from −34.10 to −28.46 mV. The emulsions stabilized by TPC-C have a decent storage stability after a high-temperature heat treatment. Overall, tea polysaccharide conjugates strongly stabilized the emulsions, which support their new application as natural emulsifiers.

Characterization of Theabrownins Prepared From Tea Polyphenols by Enzymatic and Chemical Oxidation and Their Inhibitory Effect on Colon Cancer Cells

“…The zeta potential of TBs-E was determined with a Zetasizer Nano-ZS particle diameter and potentiometric analyzer. The pH of the TBs-E aqueous solution was adjusted from 3.0 to 9.0 with solutions of 0.1 M hydrochloric acid and 0.1 M sodium hydroxide solution at an interval of 1.0 and a temperature of 25°C ( 13 , 14 ).…”

“…Among the lanes, lane 1 refers to polyphenol oxidase (PPO), lanes 2 and 3 refer to the TBs-E, and lane 4 refers to the marker proteins. solutions of 0.1 M hydrochloric acid and 0.1 M sodium hydroxide solution at an interval of 1.0 and a temperature of 25 • C (13,14).…”

A novel polysaccharide prepared from Chrysanthemum morifolium cv. Fubaiju tea and its emulsifying properties

“…The absolute potential values of emulsion droplets reflected the surface charge density and the repulsion force between droplets. Emulsion stability was positively correlated with the charges on the droplet surfaces (Chen et al ., 2019). Zeta potential is an index to measure the stability of emulsion caused by electrostatic repulsion force.…”

“…The interfacial tension of CP solution was measured by drop tensiometer (Teclis, France), according to the oil drop profile automatic analysis by using Laplace equation (Chen et al ., 2019). After injection of 10 µL oil droplet into a glass tube containing 5 mL CP emulsion, the measurement was carried out for continuous 12 000 s at 25 ℃.…”

“…High‐speed shearer was used to homogenise the 8.0wt% MCT and 92.0wt% CP solution (pH 7.0, 0.01 M) for 3 min (T18, IKA, Germany). The emulsions were then treated at a pressure of 75 MPa (Microfluidics M‐110L, USA) under high‐pressure homogeniser for three cycles (Chen et al ., 2019; 2022).…”