Emulsification and degradation of “Bunker C” fuel oil by microorganisms

Zajic, J. E.; Supplisson, Bernard

doi:10.1002/bit.260140306

Cited by 96 publications

(60 citation statements)

References 6 publications

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“…Plates were incubated at 30°C for 24 h after which the colonies were counted. Diesel fuel utilizing bacteria in the soil samples were enumerated using oil agar (OA) (Zajic and Supplisson 1972); (1.8 g K 2 HPO 4 , 1.2 g KH 2 PO 4 , 4.0 g NH 4 Cl, 0.2 g MgSo 4 Á7H2O, 0.1 g NaCl, 0.01 g FeS-O 4 Á7H 2 O, 20 g agar, 2 ml diesel fuel, 1,000 ml distilled water). The oil agar plates were incubated for 5 days at 30°C before counting the colonies.…”

Section: Samplingmentioning

confidence: 99%

Dynamics of diesel fuel degradation in contaminated soil using organic wastes

Dadrasnia

Agamuthu

2013

Int. J. Environ. Sci. Technol.

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Bioremediation is an effective measure in dealing with such contamination, particularly those from petroleum hydrocarbon sources. The effect of soil amendments on diesel fuel degradation in soil was studied. Diesel fuel was introduced into the soil at the concentration of 5 % (w/w) and mixed with three different organic wastes tea leaf, soy cake, and potato skin, for a period of 3 months. Within 84 days, 35 % oil loss was recorded in the unamended polluted soil while 88, 81 and 75 % oil loss were recorded in the soil amended with soy cake, potato skin and tea leaf, respectively. Diesel fuel utilizing bacteria counts were significantly high in all organic wastes amended treatments, ranging from 111 9 106 to 152 9 106 colony forming unit/gram of soil, as compared to the unamended control soil which gave 31 9 106 CFU/g. The diesel fuel utilizing bacteria isolated from the oil-contaminated soil belongs to Bacillus licheniformis, Ochrobactrum tritici and Staphylococcus sp. Oil-polluted soil amended with soy cake recorded the highest oil biodegradation with a net loss of 53 %, as compared to the other treatments. Dehydrogenase enzyme activity, which was assessed by 2,3,5-triphenyltetrazolium chloride technique, correlated significantly with the total petroleum hydrocarbons degradation and accumulation of CO 2 . First-order kinetic model revealed that soy cake was the best of the three organic wastes used, with biodegradation rate constant of 0.148 day -1 and half life of 4.68 days. The results showed there is potential for soy cake, potato skin and tea leaf to enhance biodegradation of diesel in oil-contaminated soil.

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Section: Samplingmentioning

confidence: 99%

Dynamics of diesel fuel degradation in contaminated soil using organic wastes

Dadrasnia

Agamuthu

2013

Int. J. Environ. Sci. Technol.

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“…The said species of aquatic phycomycetes included Brevilegnia indica, Protoachyla paradoxa, Saprolegnia bhargavi and Thraustotheca clavata. The aquatic phycomycetes were grown in mineral salt media prepared according to the pioneering method of Zajic and Supplisson (1972) with the following composition: K2HPO4=1.8g, NH4CL=4.0g, MgSO4.7H2O=0.01g, Nacl=0.1g, Agar=20g and made up with 1000mls of distilled water. Drops of streptomycin sulphate (0.3g in 1000mls of distilled water) were added to suppress bacteria growth.…”

Section: Rebaiting Of Baited Aquatic Phycomycetesmentioning

confidence: 99%

“…All the plates inoculated with test aquatic fungi and the controls without test organisms were incubated at room temperature and observed daily between 1-7 days for growth and emulsification properties according to Zajic and Supplisson (1972).…”

Section: Rebaiting Of Baited Aquatic Phycomycetesmentioning

confidence: 99%

The Abilities of Four Species of Nigerian Aquatic Phycomycetes to Utilize Petroleum and Petroleum Products as Sole Carbon Sources

Odu

C.I.C.

Bukbuk

et al. 2018

ESJ

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Studies were carried out on the abilities of four aquatic phycomycetes species isolated from crude oil polluted marine , brackish and fresh water environments in Nigeria to utilize refined Petroleum and Petroleum products as sole sources of carbon. The said species of fungi included Brevilegnia indica, Protoachyla paradoxa, Saprolegnia bhargavi and Thraustotheca clavata. They were grown on fungal culture media which contained mineral salts solution, refined petroleum, kerosene and diesel as sole sources of carbon and agar as a solidifying agent. Two concentrations of each of the resulting oil agar media were used vis 1% and 2%. The resultant medium was then used to culture each of the test fungi in triplicates. The resultant culture plates were then incubated at 25 O C and left for daily observation. The test fungi that grew on each medium were observed for their abilities to emulsify the refined petroleum or its products (diesel and kerosene). Control experiments were also set up using Malt Extract Agar medium. Brevilegnia indica grew on 1% European Scientific Journal January 2018 edition Vol.14, No.3 ISSN: 1857 -7881 (Print) e -ISSN 1857-7431 241 petroleum and diesel growth media but did not grow on kerosene medium. Protoachyla paradoxa grew minimally on diesel medium at 1% and 2% compositions. Saprolegnia bhargavi did not grow on the petroleum agar medium or petroleum products media. Thraustotheca clavata grew minimally on the kerosene medium at both 1% and 2% compositions. The four test fungi emulsified the diesel agar medium at both 1% and 2% compositions. Brevilegnia indica emulsified only petrol at 1% while Non of the isolates emulsified the kerosene medium at both 1% and 2% compositions. Oil globules were also observed in the vegetative hyphae of the test fungi which grew on the experimental culture media. This means that such fungi either degraded or accumulated the petrol or petroleum products in their systems.

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“…Test tubes containing biosurfactant solutions were incubated at different temperatures (10,20,30,40,50,60,70,80,90, 100 °C) for 1 hour, then autoclaved at 121 °C for 15 min. Test solutions were allowed to cool to room temperature before measuring surface tension.…”

Section: Stability Studiesmentioning

confidence: 99%

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Hamzah¹

2016

MJAS

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Bacillus subtilis UKMP-4M5 was isolated from a hydrocarbon contaminated site which was found to be the most potential biosurfactant producer. Isolates were grown in a mineral salt medium (MSM) supplemented with sunflower oil (1% v/v) as the main carbon source. Screening was based on both qualitative (oil spreading technique) and quantitative (emulsification index and surface tension measurement) methods. B. subtilis UKMP-4M5 produced the highest oil displacement activity with and emulsification index of 17%. It also reduced surface tension of culture medium from 58.95 to 41.75 mN/m. B. subtilis UKMP-4M5 produced biosurfactant with surface tension measurement of 32.7 ± 0.66 mN/m using 2% (v/v) palm oil and 0.5% (w/v) yeast extract as carbon and nitrogen sources respectively. The tested biosurfactant exhibited excellent thermal (up to 120 °C) and pH (6.0 -8.0) stability as well as high tolerance for varying salt concentrations (1 -5% w/v) in terms of surface tension reducing ability. This suggests potential applications in fields such as enhanced oil recovery, bioremediation, and the food industry. Keywords: Bacillus subtilis, biosurfactant, palm oil, surface tension AbstrakBacillus subtilis UKMP-4M5 yang dipencilkan dari kawasan yang tercemar dengan hidrokarbon didapati paling berpotensi sebagai penghasil biosurfaktan. Pencilan dihidupkan di dalam medium garam mineral (MSM) yang ditambahkan dengan 1% (i/i) minyak bunga matahari sebagai sumber karbon utama. Penyaringan adalah berdasarkan kepada kedua-dua kaedah kualitatif (teknik penyebaran minyak) dan kuantitatif (indeks emulsifikasi dan ukuran ketegangan permukaan). B. subtilis UKMP-4M5 merekodkan aktiviti sesaran minyak yang tertinggi dengan indeks emulsifikasi sebanyak 17%. Ia juga merendahkan ketegangan permukaan kultur medium dari 58.95 mN/m ke 41.75 mN/m B. subtilis UKMP-4M5 menghasilkan biosurfaktan dengan ukuran ketegangan permukaan 32.7 ± 0.66 mN/m menggunakan 2% (i/i) minyak kelapa sawit dan 0.5% (b/i) ekstrak yis masing-masing sebagai sumber karbon dan nitrogen. Biosurfaktan yang diuji menunjukkan kestabilan yang amat baik pada suhu (sehingga 120 °C) dan pH (6.0 -8.0) serta toleransi yang tinggi pada kepekatan garam yang berbeza (1 -5% b/i) dari segi keupayaannya merendahkan ketegangan permukaan. Ini mencadangkan potensi aplikasinya dalam industri seperti peningkatan perolehan minyak, bioremediasi dan industri makanan.

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Emulsification and degradation of “Bunker C” fuel oil by microorganisms

Cited by 96 publications

References 6 publications

Dynamics of diesel fuel degradation in contaminated soil using organic wastes

Dynamics of diesel fuel degradation in contaminated soil using organic wastes

The Abilities of Four Species of Nigerian Aquatic Phycomycetes to Utilize Petroleum and Petroleum Products as Sole Carbon Sources

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

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