Employment of pulmonary superoxide dismutase, catalase, and glutathione peroxidase activity as criteria for assessing suitable animal models for studies of bronchopulmonary dysplasia

Roberts, Robert J.

doi:10.1016/s0022-3476(79)80465-5

Cited by 12 publications

(5 citation statements)

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“…This, along with elevated concentrations of CAT, GPX, and Mn SOD may contribute to the relative tolerance to hyperoxia evident in the young of many species (22). In the fetal rat G-6-PD, GPX, CuZn SOD, and Mn SOD are all lower in d 20 lung than in d 22 (term) lung.…”

Section: Discussionmentioning

confidence: 97%

“…Successful adaptation to a hyperoxic environment by young or adult animals of a number of species is associated with increased intrapulmonary concentrations of enzymes with antioxidant protective functions (6,22).…”

mentioning

confidence: 99%

“…Subsequent observations suggest manganese SOD may be of critical importance in antioxidant defence (6,11). Reduced concentrations of pulmonary antioxidant enzymes before term could account, in part, for the increased susceptibility of prematurely born infants to BPD (8,14) whereas differences between neonatal and adult enzyme activities may contribute to the increased tolerance of newborn animals tow hyperoxic environments normally lethal to adults of the same species (22).…”

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confidence: 99%

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Pulmonary Antioxidant Enzyme Maturation in the Fetal and Neonatal Rat. I. Developmental Profiles

Tanswell

1984

Pediatr Res

138

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SummaryNeonatal, adult, and fetal rat lungs of 18,20, and 22 d gestation from four to six litters were examined for cytochrome oxidase, glucose-6-phosphate dehydrogenase, catalase, glutathione peroxidase, copper-zinc and manganese superoxide dismutase activities. All results were corrected for the contribution of enzymes in blood that contaminate homogenates. Because lung protein/ DNA ratios and body water change significantly with gestational age, enzyme activities were expressed as U/mg DNA. All activities were low in d 18 lung and increased with advancing gestational age. Only catalase and copper-zinc superoxide dismutase increased activity in response to air breathing, suggesting that maturation of the antioxidant enzyme system is virtually complete before delivery. Activities of glucose-6-phosphate dehydrogenase, catalase, glutathione peroxidase, and manganese superoxide dismutase were higher in neonatal than in adult lung. AbbreviationsBPD, bronchopulmonary dysplasia CAT, catalase CYT OX, cytochrome oxidase G-6-PD, glucose-6-phosphate dehydrogenase GPX, glutathione peroxidase HBSS, Hank's balanced salt solution SOD, superoxide dismutase Premature delivery and exposure to elevated concentrations of inspired oxygen may be significant risk factors (8,14) for the development of BPD. Successful adaptation to a hyperoxic environment by young or adult animals of a number of species is associated with increased intrapulmonary concentrations of enzymes with antioxidant protective functions (6, 22).There is evidence that the initial cell damage observed in hyperoxic tissue occurs as a consequence of increased production of intracellular oxygen radicals by cell constituents, including mitochondria and endoplasmic reticulum (1 I , 20, 29). Cell survival requires the cell either to have adequate antioxidant defense mechanisms or to rapidly respond to oxidant stress by an increases of such mechanisms that can detoxify reduced species of oxygen and by-products. Because production of superoxide and hydrogen peroxide increases in lung cells as a function of oxygen concentration (1 1, 20, 29), the 5-fold increase in arterial oxygen tension which occurs after delivery (23) would be expected to pose significant oxidant stress to the normal neonatal lung, especially since an acute 5-fold increase in inspiratory oxygen concentration is lethal to adults of most species (20). The normal term newborn human infant, however, shows no clinical evidence of pulmonary oxidant damage, suggesting that adequate antioxidant defences have been induced in the lung before delivery. Gestation-dependent increases in antioxidant enzyme activities have been reported for placenta (30) and for catalase, but not GPX or SOD (18, 34) in liver. In rat brain GPX activity is similar, at birth, to that observed in adults whereas SOD activities are low compared with the adult (19).Postnatal changes in pulmonary antioxidant enzyme activities have been comprehensively documented (5, 15, 33) whereas, to our knowledge, antenatal changes have only been studied i...

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Section: Discussionmentioning

confidence: 97%

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confidence: 99%

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confidence: 99%

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Pulmonary Antioxidant Enzyme Maturation in the Fetal and Neonatal Rat. I. Developmental Profiles

Tanswell

1984

Pediatr Res

138

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“…For example, there is incomplete development of rat alveoli at birth, followed by rapid alveolarization of terminal airways after birth. In addition, antioxidant enzyme activities are lower in the neonatal rat than in the adult rat, a characteristic similar to that in the human neonate (20). These developmental similarities make the neonatal rat a suitable model for investigating the role of Se in development of the neonatal lung under conditions of normoxia and hyperoxia.…”

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confidence: 88%

The Role of Selenium Nutrition in the Development of Neonatal Rat Lung

et al. 1991

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“…17 In infants with bronchopulmonary dysplasia the protective enzyme systems, such as superoxide dismutase and catalase, may be overwhelmed during the oxygen therapy for various acute lung disorders. 18 Measurements of the activity of superoxide dismutase in infants with hyaline membrane disease or bronchopulmonary dysplasia have been inconclusive. Analysis of the role of oxygen toxicity in causing bronchopulmonary dysplasia is complicated by the variability in patient susceptibility (perhaps related to lung immaturity) and the concentration and duration of oxygen exposure, which is a function of the severity of the underlying disease.…”

Section: Oxygen Toxicitymentioning

confidence: 99%

Bronchopulmonary Dysplasia: Challenges for Research

Jackson¹

1984

Semin Respir Crit Care Med

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Employment of pulmonary superoxide dismutase, catalase, and glutathione peroxidase activity as criteria for assessing suitable animal models for studies of bronchopulmonary dysplasia

Cited by 12 publications

References 14 publications

Pulmonary Antioxidant Enzyme Maturation in the Fetal and Neonatal Rat. I. Developmental Profiles

Pulmonary Antioxidant Enzyme Maturation in the Fetal and Neonatal Rat. I. Developmental Profiles

The Role of Selenium Nutrition in the Development of Neonatal Rat Lung

Bronchopulmonary Dysplasia: Challenges for Research

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