Employing sulphated polysaccharide (fucoidan) as medium for gold nanoparticles preparation and its anticancer study against HepG2 cell lines

et al. 2021

JPRI

Introduction: A mouthwash or rinse cannot replace the regular oral hygiene routine of twice-daily tooth brushing and daily flossing.A mouthwash or rinse freshen breath, and control the bad breath.A mouthwash is a medicated liquid which is held in the mouth and swished by the action of perioral musculature to eliminate the oral pathogens.The medicinal plants have their application in pharmaceutical, cosmetic, agricultural and food industries.Medicinal plants act as a natural source for bioactive medicinal compounds.Selenium is a vita micronutrient that has excellent antimicrobial, anti cancerous, antidiabetic, and anti-inflammatory properties.The biological method of synthesis of Selenium nanoparticles has extensive application in the field of biomedicine due to low toxicity, targeted delivery of Nano drugs and stability. Materials and Methods: Pterocarpus santalinus plant extract and mouthwash was prepared and cytotoxicity activity was performed. Results: On day 1 all the 10 nauplii were alive in all the concentrations. On day 2 when the concentration was 5, 10, 20, 40,80, control the number of live nauplii was 10,8,8,8,7,10 respectively.Results were tabulated and graphically analyzed in SPSS software. Correlation analysis was done using SPSS software. Conclusion: Increased concentration of selenium nanoparticles was not toxic to living nauplii as it did not cause the death of it.But the Pterocarpus santalinus extraction based selenium nanoparticles have less to no cytotoxicity.This study shows that mouth rinses from medicinal plants as a source of an excellent alternative with less common side-effects to chemical-based oral antimicrobial products.

Section: Discussionmentioning

confidence: 99%

Preparation and Cytotoxic Effect of Pterocarpus santalinus Selenium Based Mouthwash

Nivedha

et al. 2021

JPRI

“…The limitations of this study are that it can be done on various culture plates and observe the specific activity of each [70]. It can also be done with many bacterial and fungal organisms excluding those that have been studied in this research [71]. The future scope for this study can lead to the development of commercial products of various nanoformulations, mouthwash, toothpaste, oral gels, etc that are safe, effective, and are economical [4].…”

Section: Discussionmentioning

confidence: 99%

Green Synthesis of Zinc Oxide NPS Using Boerhavia diffusa and Its Anticariogenic Activity

Jayadharani

et al. 2021

JPRI

Introduction: Boerhavia diffusa (BD) is a plant of rasayana category as per ayurvedic claims. It is reported to possess anticariogenic activity, disease prevention, and life strengthening activities which hold enormous influence in disease burden and affordability/availability of healthcare in the world. Materials and Methods: 1g of Boerhavia diffusa is mixed 100 ml of distilled water and 2.5(0.514)g of (20 micromolar boerhavia diffusa) was dissolved in 60ml of distilled water to that 40 ml of filtered plant extract was added and was kept in an orbital shaker for approximately 72 hours. The formation of nanoparticles were confirmed both visually and by UV visible spectrophotometer. The nanoparticles were then centrifuged with the aid of a lark refrigerator centrifuge for 10 minutes at 8000 rpm. Results and Discussion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar was prepared and sterilized for 45 min at 120Ibs. The media was poured into the sterilized playesa d was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed. Conclusion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar was prepared and sterilized for 45 min at 120Ibs. The media poured into the sterilized plates was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed.

“…It has a direct and wide band gap in the near-UV spectral region, and a large free-exciton binding energy so that excitonic emission processes can persist at or even above room temperature [39,40]. Its properties have been studied since the early days of semiconductor electronics, but the use of Zinc oxide as a semiconductor in electronic devices has been hindered by the lack of control over its electrical conductivity [41,42]. Zinc oxide crystals are almost always n-type, the cause of which has been a matter of extensive debate and research .Over the past decade we have witnessed a significant improvement in the quality of Zinc oxide single-crystal substrates and epitaxial films [ (43,44].This, in turn, has led to a revival of the idea of using Zinc oxide as an optoelectronic or electronic material in its own right [45,46].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Activity of Boerhavia diffusa Zinc Oxide Nanoparticle

Jalan

et al. 2021

JPRI

Introduction: Boerhaavia diffusa is a species of flowering plant in the four o'clock family which is commonly known as punarnava, red spiderling, spreading hogweed, or tarvine. It is taken in herbal medicine for pain relief and other uses. Zinc oxide is used to treat or prevent minor skin irritations such as burns, cuts, and diaper rash. Some products may be used as sunscreen. Materials and Methods: Bovine serum albumin was used for the assay. 2ml of bovine albumin was mixed with 400micro microliter of hyaluronic acid mediated zinc nanoparticles in different concentrations. Diclofenac sodium in different concentrations was used as standard and then incubated for 55 c for 20 min and then the result was analysed spectrometrically. Results: According to the results, at 10μl, 20μl ,30μl , 40μl and 50μl concentration the percentage of inhibition is 25%, 50%, 75%, 85%, 90%, when compared to standard concentration and its percentage of inhibition is 95% . As the concentration increases, the percentage of inhibition also increases. Conclusion: From the above study, it is evident that zinc sulphate nanoparticles synthesised by Boerhevia diffusa based showed a potent anti-inflammatory effect.