Green Synthesis of Zinc Oxide NPS Using Boerhavia diffusa and Its Anticariogenic Activity

Abstract

Introduction: Boerhavia diffusa (BD) is a plant of rasayana category as per ayurvedic claims. It is reported to possess anticariogenic activity, disease prevention, and life strengthening activities which hold enormous influence in disease burden and affordability/availability of healthcare in the world.
 Materials and Methods: 1g of Boerhavia diffusa is mixed 100 ml of distilled water and 2.5(0.514)g of (20 micromolar boerhavia diffusa) was dissolved in 60ml of distilled water to that 40 ml of filtered plant extract was added and was kept in an orbital shaker for approximately 72 hours. The formation of nanoparticles were confirmed both visually and by UV visible spectrophotometer. The nanoparticles were then centrifuged with the aid of a lark refrigerator centrifuge for 10 minutes at 8000 rpm.
 Results and Discussion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar was prepared and sterilized for 45 min at 120Ibs. The media was poured into the sterilized playesa d was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed.
 Conclusion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar was prepared and sterilized for 45 min at 120Ibs. The media poured into the sterilized plates was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed.

“Elutriating therapeutic prospective of natural pharmacological agents of Renatus Nova”

“…Anticarcinogenic and Antiproliferative Activity Boerhaviadiffusa have shown cytotoxic and antiproliferative effect on various cell lines, like human cervical cancer HeLa cell line (Srivastava et al, 2009), mouse macrophage cells (RAW 264.7), human macrophage cells (U937), human monocytic cells (THP-1), mouse fibroblast cells (L929), human embryonic kidney cells (HeLa293), mouse liver cells (BNCLC.2), African green monkey kidney cell (COS-1), mouse lymphoma cells (EL-4), human erythroleukemia cells (K562), and human T cells (Mehrotra et al, 2002) [35]. Nano-technology and Boerhaviadiffusa L. Jayadharani et al,used plant of Boerhaviadiffusa L. as a catalyst in the synthesis of silver nano -particles [36]. Bairwa et al, found out that the 70% ethanol extract was used extricate nano retinoids, and all isolated nanorotenoids were evaluated for their COX-1 and COX-2 inhibitory activities.…”