Empirical Study on Grouping Behavior of Individual Endothelial Cells under Shear Stress.

Tanishita, Kazuo; Nagayama, Kazuhiro; Fujii, Madoka; Kudou, Susumu

doi:10.1299/jsmec.42.715

Cited by 5 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As just described, these changes in cytoskeleton and adherence protein need several hours and probably cause the refractory period of EC migration and network formation. For EC migration, Tanishita et al (34) also observed ECs under shear stress of 5 Pa in which migration velocity was 1-5 m/h initially and accelerated slightly after 12-16 h, finally reaching ϳ16 m/h after 16 h. In our study, we observed that ECs required ϳ10 h to transmit the effect of shear stress to not only EC migration but also network formation. Our study also revealed that the migration velocity and direction of ECs on the confluent surface depended on the distance from the network centroid (Figs.…”

Section: Effect Of Shear Stress On 3-d Network Formationsupporting

confidence: 68%

“…They suggested that the vessel is regulated to maintain a constant wall shear stress at its physiological level. Many in vitro studies using cultured ECs reported that application of fluid shear stress affects various cell functions and morphology (2,8,12,34). Thus wall shear stress is a major factor influencing the adaptive vessel regulation for physiological as well as pathophysiological processes.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model

Ueda

Koga

Ikeda

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

View full text Add to dashboard Cite

Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after approximately 10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (+/-0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (+/-0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

show abstract

Section: Effect Of Shear Stress On 3-d Network Formationsupporting

confidence: 68%

mentioning

confidence: 99%