Empedopeptin (BMY-28117), a new depsipeptide antibiotic. I. Production, isolation and properties.

Cyclic lipopeptides as antibacterial agents – Potent antibiotic activity mediated by intriguing mode of actions

“…4). Most peptides belonging to this family exhibit potent antibacterial activity against Gram-positive bacteria, including important antibiotic-resistant pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci and vancomycin-resistant enterococci (Hashizume et al, 2001;Konishi et al, 1984;Maki et al, 2001;Shoji et al, 1992).…”

Lipodepsipeptide Empedopeptin Inhibits Cell Wall Biosynthesis through Ca2+-dependent Complex Formation with Peptidoglycan Precursors

“…Production and Purification of Empedopeptin-Production of empedopeptin by E. haloabium ATCC 31962 on a 3-liter scale and extraction of the broth with butanol were performed as described (1). The crude butanol extract was subjected to reversed-phase vacuum liquid chromatography using stepwise gradient elution from H 2 4 OAc) over a period of 15 min and 65:35-90:10 over a 5-min period followed by isocratic elution at 90:10 for an additional 10 min (Macherey-Nagel Nucleosil 120-5 C 18 , 250 ϫ 4 mm, 5 m; 0.8 ml/min flow rate; UV monitoring at 210 nm) afforded 6.2 mg of pure empedopeptin.…”

“…Two accompanying publications in 1984 described its isolation, structure determination, and antibacterial activity in vitro and in vivo (1,2). Empedopeptin showed potent antibacterial activity against a broad range of aerobic and anaerobic Gram-positive bacteria including the important pathogens Staphylococcus aureus, Streptococcus pneumoniae, and Clostridium difficile.…”

Tripropeptin C Blocks the Lipid Cycle of Cell Wall Biosynthesis by Complex Formation with Undecaprenyl Pyrophosphate

“…Characteristic features of this class of antibiotics include the presence and positions of an arginine residue and two hydroxy aspartic acids. This class of antibiotics shows potent in vitro antimicrobial activity against Gram-positive pathogens, including drug-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), and also shows excellent therapeutic efficacy, comparable to that of vancomycin (VAN), the last resort for MRSA treatment, in a mouse model of staphylococcal septicemia (11,22). TPPC shows no cross-resistance to other important antibiotic-resistant strains (4,5), strongly suggesting that TPPC has a unique mode of action.…”

“…Plusbacins (22,23) and empedopeptin (11,25), which are structurally related to TPPC, are reported to have potent activity against Gram-positive bacteria (Fig. 1).…”