EMMPRIN and fascin overexpression associated with clinicopathologic parameters of pancreatobiliary adenocarcinoma in Chinese people

Tsai, Wen‐Chiuan; Chao, You‐Chen; Sheu, Lai-Fa; Lin, Yeh-Feng; Nieh, Shin; Chen, Ann; Yu, Cheng-Pin; Jin, Jong‐Shiaw

doi:10.1111/j.1600-0463.2007.apm_858.x

Cited by 30 publications

(39 citation statements)

References 21 publications

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“…Previous studies of colon cancer by Davidson [7] and Tsai [8] proved that EMMPRIN played a key role in prognostic of colon carcinoma and pancreatic cancer. On the other hand, studies of liver cancer founded that EMMPRIN did not express in normal liver tissue but expressed in all liver cancer cells and related to liver cancer metastasis and clinical analysis [9] .…”

Section: Discussionmentioning

confidence: 99%

EMMPRIN enhances the metastatic ability of CT26 murine colon adenocarcinoma

Dai

Tian

Tan

et al. 2011

Chin. -Ger. J. Clin. Oncol.

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Colon cancer metastasis is the biological characters of colon cancer, and also lethal factor of most colon cancer patients. Primary colon cancer can be healed by surgical removal or radiotherapy, but for colon cancer which had spread; it is difficult to get ideal effects through above methods. Colon cancer metastasis or not has key impact on efficacy and prognosis, which is central difficulty on therapy of colon cancer. For the reasons, there are mainly two: (1) heterogeneous of malignant tumor; (2) different host factors and diversity of in vivo conditions. Extracellular matrix metalloproteinase inducer (EMMPRIN) was first purified from human lung cancer cell LX-1 by Ellis at 1989 [1] , and was named tumor cellderived collagenase stimulatory factor (TSCF). Later research found that it is also expressed in normal human tissues. For the function of evidently inducing matrix metalloproteinase, it is renamed EMMPRIN by Biswas [2] at 1995.At normal physical conditions, EMMPRIN extensive expressed in normal human tissues, such as horniness cells, embryo epithelia cells, vas endodermis cells and so on. It formed human blood brain barrier and participated in many physiological process like wound heal, tissue repair, gestation and fetation [3] . In pathological state like malignant tumor pathological process, EMMPRIN basically participate in adhesion between cell and cell or cell and matrix. In vitro experiment found that EMMPRIN from tumor cell can stimulate human fibroblasts and endothelial cells secreting MMPs and engage in damaging natural organization mechanical barrier helping cancer cell invasion and spread [4][5][6] .In order to define the role of EMMPRIN in colon cancer metastasis, we constructed the eukaryotic expression vector pCMV-HA2-EMMPRIN to transfect colon cancer cell line CT26 and observed the effect to CT26 metastasis. Materials and methods MaterialsCell Lines and plasmids CT26 murine colon carcinoma cell were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. Eukaryotic expression vector pCMV-HA2 was from our library. Main reagent and instrumentAbstract Objective: Colon cancer metastasis is the key in fertility rate of colon cancer. Many recent results about metastasis research indicated that EMMPRIN played an important role in cancer metastasis. So, we designed this experiment to investigate whether EMMPRIN can enhance the metastatic ability of murine colon adenocarcinoma cell, CT26. Methods: EMMPRIN was over expressed in CT26 cells through transfecting pCMV-HA2-EMMPRIN into the CT26 cells. Invasion assay, wound migration assay and adhesion assay were utilized to analyze the metastasis of CT26 cells in vitro after EMMPRIN over expression. Results: After EMMPRIN over expression, invasion assay showed that invasive cells were 103.33 + 8.49 in EMMPRIN group and 48.67 + 5.3 in control group (P < 0.001). Migration assay showed that migrating cells were 40.67 + 2.49 in EMMPRIN group and 18.33 + 2.05 in control group (P < 0.001). CCK-8 absorbance value in adhesion assay were 3.33 + 0.17 ...

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Section: Discussionmentioning

confidence: 99%

EMMPRIN enhances the metastatic ability of CT26 murine colon adenocarcinoma

Dai

Tian

Tan

et al. 2011

Chin. -Ger. J. Clin. Oncol.

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“…For these reasons, fascin and EMMPRIN expression have been investigated for many tumors and revealed to be important for tumor progress and metastasis ability. Their presence is usually associated with poor prognostic and clinicopathological features [13][14][15][16][17][20][21][22][23][24][25][26]. For lung carcinomas, there have been few studies of fascin and EMMPRIN expression [27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…MMPs have been shown to be related to degradation of the extracellular matrix, angiogenesis and tumor progression [11,12]. Therefore, elevated EMMPRIN expression is reported for some tumors and its presence correlates with their poor clinical and pathologic features [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

EMMPRIN and fascin expression in non-small cell lung carcinoma

et al. 2010

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AbstractFascin and EMMPRIN (CD 147) have a demonstrated relationship with the invasion and progression of many tumors. The aim of this study was to analyze fascin and EMMPRIN expression in non-small cell lung carcinoma and their relationship with clinicopathologic features. Fascin and EMMPRIN expression levels were investigated via the immunohistochemistry of paraffin-embedded tissues of 64 patients with non-small cell lung carcinoma, including 46 squamous cell carcinoma and 18 adenocarcinoma patients. The patients were scored on the basis of staining extent and intensity, and were then assigned a combined score. Fascin expression was present in 44 of 46 (95.6%) patients with SCC and 16 of 18 (88.8%) patients with adenocarcinoma. There was a significant correlation between fascin expression and tumor stage in the SCC and adenocarcinoma groups. EMMPRIN expression was observed in all patients with SCC (46 of 46, 100%) and 16 of 18 (88.8%) patients with adenocarcinoma. There was significant correlation between EMMPRIN expression and both tumor stage and diameter in the SCC group, but not in the adenocarcinoma group. This study revealed that high levels of fascin and EMMPRIN expression may indicate the importance of their roles in the progression of non-small cell lung carcinoma and they could be used as prognostic marker for these tumors.

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“…The growth and invasion of GC cells involves the breakdown of the extracellular matrix by proteinases including matrix metalloproteinases (MMPs), which are involved in tumor progression, growth, invasion, angiogenesis and metastasis (12,13). Previous studies have demonstrated that Basigin promotes tumor cell invasion by stimulating stromal cells to produce elevated levels of MMPs (14)(15)(16).…”

Section: Discussionmentioning

confidence: 99%

Tumor-enhancing activity of Basigin expression in gallbladder carcinoma and its prognostic role

Tang¹

2009

Mol Med Rep

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Abstract. Basigin (EMMPRIN/CD147) is a multifunctional membrane glycoprotein that is overexpressed in many solid tumors and is involved in tumor invasion and angiogenesis. The main purpose of this study was to investigate the tumorenhancing activity of Basigin in a gallbladder carcinoma (GC) cell line and in primary GC tissues. A system that blocks Basigin in the human primary GC cell line GBC-SD was developed using RNA interference. GBC-SD cells were transfected with the small interfering RNA that target Basigin, then the proliferative, invasive and migration activities of the cells were assayed in vitro. Additionally, tissue samples from 98 patients with GC and 26 patients with chronic cholecystitis were stained with anti-Basigin antibody for immunohistochemical analysis. Furthermore, the association of Basigin expression with the clinicopathological characteristics and prognosis of the patients was analyzed. siRNA-treated GBC-SD cells exhibited significantly decreased growth ability, invasion and migration capacities compared to control cells in vitro. Moreover, clinicopathological analysis demonstrated that the intensity of Basigin staining in cancerous tissue was significantly associated with the histological type (p=0.02), distant metastasis (p<0.01) and Nevin stage (p<0.01) of GC. A proportional hazards model revealed the survival rate of patients with stronger Basigin expression to be the lowest (p<0.01). These results suggest that Basigin is a prognostic marker and potential therapeutic target for patients with GC.

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EMMPRIN and fascin overexpression associated with clinicopathologic parameters of pancreatobiliary adenocarcinoma in Chinese people

Cited by 30 publications

References 21 publications

EMMPRIN enhances the metastatic ability of CT26 murine colon adenocarcinoma

EMMPRIN enhances the metastatic ability of CT26 murine colon adenocarcinoma

EMMPRIN and fascin expression in non-small cell lung carcinoma

Tumor-enhancing activity of Basigin expression in gallbladder carcinoma and its prognostic role

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