EMMPRIN enhances the metastatic ability of CT26 murine colon adenocarcinoma

Abstract

Colon cancer metastasis is the biological characters of colon cancer, and also lethal factor of most colon cancer patients. Primary colon cancer can be healed by surgical removal or radiotherapy, but for colon cancer which had spread; it is difficult to get ideal effects through above methods. Colon cancer metastasis or not has key impact on efficacy and prognosis, which is central difficulty on therapy of colon cancer. For the reasons, there are mainly two: (1) heterogeneous of malignant tumor; (2) different host factors and diversity of in vivo conditions. Extracellular matrix metalloproteinase inducer (EMMPRIN) was first purified from human lung cancer cell LX-1 by Ellis at 1989 [1] , and was named tumor cellderived collagenase stimulatory factor (TSCF). Later research found that it is also expressed in normal human tissues. For the function of evidently inducing matrix metalloproteinase, it is renamed EMMPRIN by Biswas [2] at 1995.At normal physical conditions, EMMPRIN extensive expressed in normal human tissues, such as horniness cells, embryo epithelia cells, vas endodermis cells and so on. It formed human blood brain barrier and participated in many physiological process like wound heal, tissue repair, gestation and fetation [3] . In pathological state like malignant tumor pathological process, EMMPRIN basically participate in adhesion between cell and cell or cell and matrix. In vitro experiment found that EMMPRIN from tumor cell can stimulate human fibroblasts and endothelial cells secreting MMPs and engage in damaging natural organization mechanical barrier helping cancer cell invasion and spread [4][5][6] .In order to define the role of EMMPRIN in colon cancer metastasis, we constructed the eukaryotic expression vector pCMV-HA2-EMMPRIN to transfect colon cancer cell line CT26 and observed the effect to CT26 metastasis. Materials and methods MaterialsCell Lines and plasmids CT26 murine colon carcinoma cell were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. Eukaryotic expression vector pCMV-HA2 was from our library. Main reagent and instrumentAbstract Objective: Colon cancer metastasis is the key in fertility rate of colon cancer. Many recent results about metastasis research indicated that EMMPRIN played an important role in cancer metastasis. So, we designed this experiment to investigate whether EMMPRIN can enhance the metastatic ability of murine colon adenocarcinoma cell, CT26. Methods: EMMPRIN was over expressed in CT26 cells through transfecting pCMV-HA2-EMMPRIN into the CT26 cells. Invasion assay, wound migration assay and adhesion assay were utilized to analyze the metastasis of CT26 cells in vitro after EMMPRIN over expression. Results: After EMMPRIN over expression, invasion assay showed that invasive cells were 103.33 + 8.49 in EMMPRIN group and 48.67 + 5.3 in control group (P < 0.001). Migration assay showed that migrating cells were 40.67 + 2.49 in EMMPRIN group and 18.33 + 2.05 in control group (P < 0.001). CCK-8 absorbance value in adhesion assay were 3.33 + 0.17 ...