Emerging ThermotolerantCampylobacterSpecies in Healthy Ruminants and Swine

Abstract

Campylobacters other than Campylobacter jejuni or C. coli were isolated in 35% of 343 farms recently analyzed in northern Spain. This study was aimed at identifying at the species level the 120 isolates collected (21 ovine, 52 beef cattle, 44 dairy cattle, and 3 porcine) by species-specific polymerase chain reaction and 16S rRNA gene sequencing analysis. Thus, five species were identified: Campylobacter hyointestinalis (90 isolates), Campylobacter lanienae (12), Campylobacter fetus subsp. fetus (10), Campylobacter lari (1), and Campylobacter sputorum (1). Ambiguous results were obtained for six isolates. Phylogenetic analyses of the 16S rRNA gene sequence placed three of them (cattle isolates) as an intermediate clade between C. hyointestinalis subsp. hyointestinalis and C. fetus, two ovine isolates formed a new clade clustering with Campylobacter concisus despite sharing higher similarity with Campylobacter mucosalis, and one porcine isolate shared similarly high homology with C. lanienae and C. hyointestinalis subsp. lawsonii. C. hyointestinalis was the predominant species, particularly in cattle, but it was also isolated from sheep and swine. C. lanienae was only found in sheep, C. fetus in cattle and sheep, and C. lari in a single dairy cattle farm. Although previously reported, the isolation of C. lari from cattle is not common, and this is the first report of C. lanienae and C. hyointestinalis in sheep. This study demonstrated the wide distribution in livestock of several emerging zoonotic Campylobacter species and provided valuable information on their host animal reservoirs.

Prevalence and Antimicrobial Susceptibility of Foodborne Bacteria in Wild Boars (Sus scrofa) and Wild Deer (Cervus nippon) in Japan

“…In that study, C. lanienae was isolated from the feces of healthy abattoir workers in Switzerland. Although C. lanienae has occasionally been isolated from pigs (Schweitzer et al, 2011), cattle (Inglis et al, 2006), and sheep (Oporto and Hurtado, 2011) in countries other than Japan, this bacterium is a minor species of campylobacters in these animals. The primary source of C. lanienae remains unknown.…”

Factores asociados a la contaminación con <i>Campylobacter</i> spp. termotolerante en pollos de engorde, en tres niveles de la cadena avícola, para consumo humano en Costa Rica.

“…a la carne de aves, canales de cerdo y de res (Oporto & Hurtado 2011, Abley et al 2012, Mattheus et al 2012, por lo cual, la contaminación cruzada, al existir contacto directo con carne de diferentes orígenes en el punto de venta, es altamente probable; factor que se demostró en este estudio cuando no había separación física entre las carnes (RP 1.77).…”

A new variant of cytolethal distending toxin in a clinical isolate of Campylobacter hyointestinalis

“…Identification of CDT in C. hyointestinalis C. hyointestinalis was isolated and detected from various animals, including pig (Gebhart et al, 1983(Gebhart et al, , 1985(Gebhart et al, , 1990Gorkiewicz et al, 2002), cattle (Gebhart et al, 1985;Oporto & Hurtado, 2011), dog (Chaban et al, 2010), hamster (Gebhart et al, 1985), monkey (Russell et al, 1992), sheep (Oporto & Hurtado, 2011), deer (Hill et al, 1987) and reindeer (Hänninen et al, 2002). Based on these findings C. hyointestinalis was thought to be a pathogen to animals, in particular pigs.…”