Emerging strategies for RNA interference (RNAi) applications in insects

Nandety, Raja Sekhar; Kuo, Yen-Wen; Nouri, Shahideh; Falk, Bryce W.

doi:10.4161/21655979.2014.979701

Cited by 64 publications

(57 citation statements)

References 134 publications

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“…Recently, plant viruses have been investigated as tools to trigger RNAi in plants (Khan et al, 2013; Wuriyanghan and Falk, 2013; Nandety et al, 2015). Generally, plants respond to infections caused by viruses through the siRNAi pathway (vsRNAs).…”

Section: Novel Delivery Methodsmentioning

confidence: 99%

“…Generally, plants respond to infections caused by viruses through the siRNAi pathway (vsRNAs). Therefore, if an insect-specific RNAi inducer sequence is introduced into an engineered plant virus, this will produce siRNAs in the plant that are specific for insect targets (Nandety et al, 2015). The RNAi effect can be induced when insects feed on plants containing engineered virus to produced specific siRNAs.…”

Section: Novel Delivery Methodsmentioning

confidence: 99%

“…Here, it is important to remember that dsRNA can induces RNAi through siRNAs, and afterwards yielding a wide range of siRNAs which might influence the potential for off target effects. The processing of dsRNA does not occur at fixed/phased ~21 nt intervals (Nandety et al, 2015). The dsRNA sequence recognized as foreign can be cleaved by Dicer beginning at basically any nucleotide, generating a number of potentially overlapping siRNA ranging in size and sequence that are further populated by other size siRNAs generated by the RNAi mechanism.…”

Section: What Matters For Rnai Efficiency?mentioning

confidence: 99%

“…RNAi technology has been an effective tool in functional genomics studies and its application toward pest management is already close to a reality (Kupferschmidt, 2013; Palli, 2014; Saurabh et al, 2014; Nandety et al, 2015; Sherman et al, 2015; Zotti and Smagghe, 2015; Andrade and Hunter, 2016). The effectiveness of the RNAi mechanism is mainly depending on the delivery, stability, and uptake of dsRNA by target species.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

et al. 2016

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In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches toward insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of dsRNA through non-transformative methods. We discuss factors influencing the RNAi mechanism in insects and systemic properties of dsRNA. Finally, novel strategies to deliver dsRNA are discussed, including delivery by symbionts, plant viruses, trunk injections, root soaking, and transplastomic plants.

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Section: Novel Delivery Methodsmentioning

confidence: 99%

Section: Novel Delivery Methodsmentioning

confidence: 99%

Section: What Matters For Rnai Efficiency?mentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

See 2 more Smart Citations

RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

et al. 2016

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“…These RISCs recognize complementary sequences of corresponding genes and induce specific gene silencing by degrading mRNAs or inhibiting translation [9]. Similar strategies based on the specific blocking of parasitism genes by host-derived dsRNAs have also been reported to show promising effects on controlling various pathogens such as viruses, fungi, and insects [10,11,12,13]. …”

Section: Introductionmentioning

confidence: 99%

Host-Derived Artificial MicroRNA as an Alternative Method to Improve Soybean Resistance to Soybean Cyst Nematode

Tian

Oakley

et al. 2016

Genes

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The soybean cyst nematode (SCN), Heterodera glycines, is one of the most important pests limiting soybean production worldwide. Novel approaches to managing this pest have focused on gene silencing of target nematode sequences using RNA interference (RNAi). With the discovery of endogenous microRNAs as a mode of gene regulation in plants, artificial microRNA (amiRNA) methods have become an alternative method for gene silencing, with the advantage that they can lead to more specific silencing of target genes than traditional RNAi vectors. To explore the application of amiRNAs for improving soybean resistance to SCN, three nematode genes (designated as J15, J20, and J23) were targeted using amiRNA vectors. The transgenic soybean hairy roots, transformed independently with these three amiRNA vectors, showed significant reductions in SCN population densities in bioassays. Expression of the targeted genes within SCN eggs were downregulated in populations feeding on transgenic hairy roots. Our results provide evidence that host-derived amiRNA methods have great potential to improve soybean resistance to SCN. This approach should also limit undesirable phenotypes associated with off-target effects, which is an important consideration for commercialization of transgenic crops.

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Knockdown of Dcr1 and Dcr2 limits the lethal effect of C‐factor in Chilo suppressalis

Yao

Jin

Yan

et al. 2023

Arch Insect Biochem Physiol

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Dicer is a highly conserved ribonuclease in evolution. It belongs to the RNase III family and can specifically recognize and cleave double‐stranded RNA (dsRNA). In this study, the genome and transcriptome of Chilo suppressalis were analyzed, and it was found that there were two members in the Dicer family, named Dcr1 and Dcr2. The dsRNAs of Dcr1 and Dcr2 genes were synthesized and fed to C. suppressalis larvae. The C‐factor of C. suppressalis was selected as the marker gene. The results showed that both Dcr1 and Dcr2 genes were significantly knocked down. The larval mortality was significantly reduced by 43.50% (p < 0.05) after feeding on dsC‐factor and dsDcr1. The transcription levels of C‐factor genes were significantly increased by 33.95% (p < 0.05) and 32.94% (p < 0.05) when the larvae fed with dsDcr2 + dsC‐factor for 72 h and 96 h, respectively. Furthermore, the mortality was significantly decreased by 79% (p < 0.05) after feeding dsC‐factor and dsDcr2. These findings imply that Dcr1 can decrease the lethal effect of C‐factor gene but cannot affect its RNAi efficiency and Dcr2 can decrease the lethal effect of C‐factor gene by inhibiting RNAi efficiency.

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Emerging strategies for RNA interference (RNAi) applications in insects

Cited by 64 publications

References 134 publications

RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

Host-Derived Artificial MicroRNA as an Alternative Method to Improve Soybean Resistance to Soybean Cyst Nematode

Knockdown of Dcr1 and Dcr2 limits the lethal effect of C‐factor in Chilo suppressalis

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