Emerging nonsurgical methods for the treatment of vitreomacular adhesion: a review

Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin

“…Ocriplasmin includes just the enzymatic section of plasmin, whereas the other parts are deleted. Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues 22 . Commercially, ocriplasmin is produced using yeast Pichia pastoris.…”

“…Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues. 22 Commercially, ocriplasmin is produced using yeast P. pastoris . Since ocriplasmin does not contain any O- or N-glycosylation or some other post-translational modifications, E. coli can be considered as an economical and simple host for recombinant expression.…”

Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin

“…Ocriplasmin includes just the enzymatic section of plasmin, whereas the other parts are deleted. Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues 22 . Commercially, ocriplasmin is produced using yeast Pichia pastoris.…”

“…Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues. 22 Commercially, ocriplasmin is produced using yeast P. pastoris . Since ocriplasmin does not contain any O- or N-glycosylation or some other post-translational modifications, E. coli can be considered as an economical and simple host for recombinant expression.…”

Profile of ocriplasmin and its potential in the treatment of vitreomacular adhesion

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Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin

“…Ocriplasmin includes just the enzymatic section of plasmin, whereas the other parts are deleted. Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues 22 . Commercially, ocriplasmin is produced using yeast Pichia pastoris.…”

“…Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues. 22 Commercially, ocriplasmin is produced using yeast P. pastoris . Since ocriplasmin does not contain any O- or N-glycosylation or some other post-translational modifications, E. coli can be considered as an economical and simple host for recombinant expression.…”

Profile of ocriplasmin and its potential in the treatment of vitreomacular adhesion

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Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin

“…Ocriplasmin includes just the enzymatic section of plasmin, whereas the other parts are deleted. Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues 22 . Commercially, ocriplasmin is produced using yeast Pichia pastoris.…”

“…Due to smaller size of ocriplasmin compared to the original molecule (27 kDa versus 80 kDa,) when retaining the similar enzymatic activity, theoretically may provide better permeation into epiretinal tissues. 22 Commercially, ocriplasmin is produced using yeast P. pastoris . Since ocriplasmin does not contain any O- or N-glycosylation or some other post-translational modifications, E. coli can be considered as an economical and simple host for recombinant expression.…”

Profile of ocriplasmin and its potential in the treatment of vitreomacular adhesion

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