Emerging nanolabels-based immunoassays: Principle and applications in food safety

Pan, Ruiyuan; Li, Guoliang; Liu, Shucheng; Zhang, Xianlong; Liu, Jianghua; Su, Zhuoqun; Wu, Yongning

doi:10.1016/j.trac.2021.116462

Cited by 27 publications

(13 citation statements)

References 241 publications

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“…Because of the advantages of simple preparation, low cost and many types of signal output, nanomaterials can be used as an excellent signal indicator to couple with antibodies or antigens to form signal probes, which opens up a new way for the construction of immunosensors. 62 As shown in Fig. 9, Cao et al , 63 constructed a novel fluorescent immunosensor for sensitive detection of PSA based on Förster resonance energy transfer between nitrogen and sulfur co-doped carbon quantum dots functionalized silica nanospheres (Si/NS-CDs) and Au@AgNPs.…”

Section: The Role Of Nanomaterials In Immunosensormentioning

confidence: 99%

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

et al. 2022

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Section: The Role Of Nanomaterials In Immunosensormentioning

confidence: 99%

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

et al. 2022

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“…Immunological assays use antibody-antigen reactions for detecting the target viral protein (antigen) or immune response (antibody) against the SARS-CoV-2 [ 9 ]. Immunoassays have been miniaturized in several formats such as lateral flow device and electrochemical sensor at point-of-care (POC) diagnostics [ 10 ]. For example, Grant et al developed a half-strip lateral flow assay for SARS-CoV-2 antigen detection [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Label free electrochemical DNA biosensor for COVID-19 diagnosis

Lomae

Preechakasedkit

Hanpanich

et al. 2023

Talanta

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“…Through specific biotin–streptavidin affinity, the PB NP-tagged ssDNA was anchored on the microplate, and with the engagement of target DNA, the activated CRISPR/Cas12a system would efficiently cleave the linker ssDNA, making a corresponding number of PB NPs suspended in the solution. Notably, the PB NPs in this assay have advantages like regular shape, easy modification, simple synthesis procedure, and a great target-responsive function . Under alkali condition, the breakdown of PB NPs would be triggered, releasing massive Fe(CN) 6 4– .…”

Section: Introductionmentioning

confidence: 99%

“…Notably, the PB NPs in this assay have advantages like regular shape, easy modification, simple synthesis procedure, and a great target-responsive function. 21 Under alkali condition, the breakdown of PB NPs would be triggered, releasing massive Fe(CN) 6 4− . The special CN structure of Fe(CN) 6 4− exhibits a unique characteristic Raman peak that is located in the "biological Raman-silent region" (1800−2800 cm −1 ).…”

Section: ■ Introductionmentioning

confidence: 99%

Ultrasensitive CRISPR/Cas12a-Driven SERS Biosensor for On-Site Nucleic Acid Detection and Its Application to Milk Authenticity Testing

Pan

Liu

Wang

et al. 2022

J. Agric. Food Chem.

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An ultrasensitive surface-enhanced Raman scattering (SERS) biosensor driven by CRISPR/Cas12a was proposed for on-site nucleic acid detection. We tactfully modified single-strand DNA (ssDNA) with a target-responsive Prussian blue (PB) nanolabel to form a probe and fastened it in the microplate. Attributed to the specific base pairing and highly efficient trans-cleavage ability of the CRISPR/Cas12a effector, precise target DNA recognition and signal amplification can be achieved, respectively. In the presence of target DNA, trans-cleavage towards the probe was activated, leading to the release of a certain number of PB nanoparticles (NPs). Then, these free PB NPs would be removed. Under alkali treatment, the breakdown of the remaining PB NPs in the microplate was triggered, producing massive ferricyanide anions (Fe(CN) 6 4− ), which could exhibit a unique characteristic Raman peak that was located in the "biological Raman-silent region". By mixing the alkali-treated solution with the SERS substrate, Au@Ag core−shell NP, the concentration of the target DNA was finally exhibited as SERS signals with undisturbed background, which can be detected by a portable Raman spectrometer. Importantly, this strategy could display an ultralow detection limit of 224 aM for target DNA. Furthermore, by targeting cow milk as the adulterated ingredient in goat milk, the proposed biosensor was successfully applied to milk authenticity detection.

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Emerging nanolabels-based immunoassays: Principle and applications in food safety

Cited by 27 publications

References 241 publications

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Label free electrochemical DNA biosensor for COVID-19 diagnosis

Ultrasensitive CRISPR/Cas12a-Driven SERS Biosensor for On-Site Nucleic Acid Detection and Its Application to Milk Authenticity Testing

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