Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling

Matta, Leann Lerie; Alocilja, Evangelyn C.

doi:10.1016/j.bios.2018.07.007

Cited by 9 publications

(9 citation statements)

References 125 publications

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“…Nonspecific interactions of nanoparticles with serum components are inevitable when their mixing takes place during the assay procedure. Matrix interference is a significant issue in magnetic nanoparticle-based biosensing, causing a dramatic decrease in specificity [75]. According to recent reports, protein shells can provide antifouling properties by decreasing the amount of nonspecific binding of cells and medium components, first of all, serum proteins [65,76,77].…”

Section: Resultsmentioning

confidence: 99%

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

et al. 2019

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The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable of increasing the stability, biocompatibility, and transverse relaxivity (r2) of magnetic nanoparticles. In this work, we functionalized the nanoclusters of carbon-coated iron nanoparticles with four proteins: bovine serum albumin, casein, and gelatins A and B, and we conducted a comprehensive comparative study of their properties essential to applications in biosensing. First, we examined the influence of environmental parameters on the size of prepared nanoclusters and synthesized protein-coated nanoclusters with a tunable size. Second, we showed that protein coating does not significantly influence the r2 relaxivity of clustered nanoparticles; however, the uniform distribution of individual nanoparticles inside the protein coating facilitates increased relaxivity. Third, we demonstrated the applicability of the obtained nanoclusters in biosensing by the development of a nuclear-magnetic-resonance-based immunoassay for the quantification of antibodies against tetanus toxoid. Fourth, the protein coronas of nanoclusters were studied using SDS-PAGE and Bradford protein assay. Finally, we compared the colloidal stability at various pH values and ionic strengths and in relevant complex media (i.e., blood serum, plasma, milk, juice, beer, and red wine), as well as the heat stability, resistance to proteolytic digestion, and shelf-life of protein-coated nanoclusters.

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Section: Resultsmentioning

confidence: 99%

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

et al. 2019

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“…Thus, they are excellent tools for efficient capture of cells, proteins, and biomolecules. MNPs can easily be suspended in solutions for bacterial capture before separation from the supernatant using a magnet [ 53 , 54 ].…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

“…Lectins have broad specificities for complex carbohydrates [ 68 ]. Thus, magnetic nanoparticles coated with glycans can bind to various bacteria cells through non-covalent electrostatic interactions with protein residues on the bacteria surface, effectively mimicking the role of cell surface glycans [ 53 , 63 ]. Due to the superparamagnetic properties of the MNPs, the MNP-bacteria complexes can then be manipulated by an external magnetic field, allowing for rapid extraction of the bacteria [ 23 ].…”

Section: Carbohydrate Functionalized (Glycan-coated) Magnetic Separationmentioning

confidence: 99%

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Dester

Alocilja

2022

Biosensors

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Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the use of glycan-coated magnetic nanoparticles (MNPs) is often omitted. This review seeks to analyze the potential of this technique as a rapid and cost-effective solution for concentration of bacteria directly from foods. The primary focus is the mechanism of glycan-coated MNP binding, as well as its current applications in concentration of foodborne pathogens. First, a background on the synthesis, properties, and applications of MNPs is provided. Second, synthesis of glycan-coated particles and their theorized mechanism for bacterial adhesion is described. Existing research into extraction of bacteria directly from food matrices is also analyzed. Finally, glycan-coated MNPs are compared to the magnetic separation technique of immunomagnetic separation (IMS) in terms of cost, time, and other factors. At its current state, glycan-coated MNPs require more research to fully identify the mechanism, potential for optimization, and extraction capabilities directly in food matrices. However, current research indicates glycan-coated MNPs are an incredibly cost-effective method for rapid food pathogen extraction and concentration.

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“…Apart from that, m-NPs are characterised by other particular features such as high electrical conductivity, coercivity, high magnetic susceptibility and, especially, superparamagnetism that favour their separation from the samples, simplifying the procedures, shortening times and, consequently, reducing costs [1,9]. These characteristics make them ideal for the preparation of sorbents in microextraction techniques or for clean-up steps [8,10,11] and as materials for the preparation of sensors [1,10,12]. In this respect, their use in magnetic-micro-dispersive solid-phase extraction (m-µ-dSPE) has constituted their main application, while their utilisation in optical and electrochemical sensors has involved the main uses in these approaches, although other novel systems have also been described in the literature.…”

Section: Introductionmentioning

confidence: 99%

“…There are several types of m-NPs, including alloys and oxides involving both iron and cobalt, fundamentally (i.e., FePt, CoPt 3 , Fe 3 O 4 , Fe 2 O 3 , CoFe 2 O 4 , etc.). However, the most widely applied so far have been magnetite (Fe 3 O 4 ) based NPs [9,12]. For their preparation, a great variety of procedures, such as co-precipitation, thermal decomposition, micro-emulsion, or hydrothermal synthesis, has been applied.…”

Section: Introductionmentioning

confidence: 99%

Recent Applications of Magnetic Nanoparticles in Food Analysis

et al. 2020

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Nanotechnology has become a topic of interest due to the outstanding advantages that the use of nanomaterials offers in many fields. Among them, magnetic nanoparticles (m-NPs) have been one of the most widely applied in recent years. In addition to the unique features of nanomaterials in general, which exclusively appear at nanoscale, these present magnetic or paramagnetic properties that result of great interest in many applications. In particular, in the area of food analysis the use of these nanomaterials has undergone a considerable increase since they can be easily separated from the matrix in sorbent-based extractions, providing a considerable simplification of the procedures. This allows reducing cost and giving fast responses, which is essential in the food trade to guarantee consumer safety. These materials can also be easily tunable, providing higher selectivity. Moreover, their particular electrical, thermal and optical characteristics allow enhancing sensor signals, increasing the sensitivity of the approaches based on this type of device. The aim of this review article is to summarise the most remarkable applications of m-NPs in food analysis in the last five years (2016–2020) showing a general view of the use of such materials in the field.

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Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling

Cited by 9 publications

References 125 publications

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Recent Applications of Magnetic Nanoparticles in Food Analysis

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