Emerging Biomimetic Applications of DNA Nanotechnology

Shen, Haijing; Wang, Yingqian; Wang, Jie; Li, Zhihao; Yuan, Quan

doi:10.1021/acsami.8b06175

Cited by 51 publications

(46 citation statements)

References 138 publications

(239 reference statements)

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“…Interfacing DNA nanostructures with biological systems has gained significant interest in recent years, due to the ability to design arbitrary shapes with immense structural control combined with the relative ease of modifying DNA with functional groups in a sequence-programmable manner (1,2). Particularly, studying and mimicking biological processes at cell membranes or model lipid bilayers has become a popular and successful application of DNA nanotechnology (3,4).…”

Section: Introductionmentioning

confidence: 99%

Controlling aggregation of cholesterol-modified DNA nanostructures

Ohmann

Göpfrich

Joshi

et al. 2019

Nucleic Acids Research

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DNA nanotechnology allows for the design of programmable DNA-built nanodevices which controllably interact with biological membranes and even mimic the function of natural membrane proteins. Hydrophobic modifications, covalently linked to the DNA, are essential for targeted interfacing of DNA nanostructures with lipid membranes. However, these hydrophobic tags typically induce undesired aggregation eliminating structural control, the primary advantage of DNA nanotechnology. Here, we study the aggregation of cholesterol-modified DNA nanostructures using a combined approach of non-denaturing polyacrylamide gel electrophoresis, dynamic light scattering, confocal microscopy and atomistic molecular dynamics simulations. We show that the aggregation of cholesterol-tagged ssDNA is sequence-dependent, while for assembled DNA constructs, the number and position of the cholesterol tags are the dominating factors. Molecular dynamics simulations of cholesterol-modified ssDNA reveal that the nucleotides wrap around the hydrophobic moiety, shielding it from the environment. Utilizing this behavior, we demonstrate experimentally that the aggregation of cholesterol-modified DNA nanostructures can be controlled by the length of ssDNA overhangs positioned adjacent to the cholesterol. Our easy-to-implement method for tuning cholesterol-mediated aggregation allows for increased control and a closer structure–function relationship of membrane-interfacing DNA constructs — a fundamental prerequisite for employing DNA nanodevices in research and biomedicine.

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Section: Introductionmentioning

confidence: 99%

Controlling aggregation of cholesterol-modified DNA nanostructures

Ohmann

Göpfrich

Joshi

et al. 2019

Nucleic Acids Research

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“…The addressability and programmability of DNA nanostructures make it a promising candidate to mimic the structures and functions of membrane proteins. [15][16][17] On the other hand, many strategies have also been developed to prepare artificial amphiphilic membranes to mimic the natural lipid bilayer in the natural membrane systems. [18][19][20][21][22] Combining these two powerful tools, applying DNA nanostructures on amphiphilic membranes would allow the preparation of the artificial membrane model which facilitates the investigation of complicated membrane biology issues in a simple way.…”

Section: Introductionmentioning

confidence: 99%

“…While the intersection of DNA nanotechnology and amphiphilic membranes have also been reviewed and discussed elsewhere, [16,[23][24][25][26][27] this review aims to provide a comprehensive understanding of the latest developments in the application of DNA nanostructures-amphiphilic membrane to mimic biological membrane systems.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

Piao

Yuan

Dong

2021

Macromolecular Bioscience

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Employing DNA nanostructures mimicking membrane proteins on artificial amphiphilic membranes have been widely developed to understand the structures and functions of the natural membrane systems. In this review, the recent developments in artificial systems constructed by amphiphilic membranes and DNA nanostructures are summarized. First, the preparations and properties of the amphipathic bilayer models are introduced. Second, the interactions are discussed between the membrane and the DNA nanostructures, as well as their coassembly behaviors. Next, the alternative systems related to membrane protein‐mediated signal transmission, selective distribution, transmembrane channels, and membrane fusion are also introduced. Moreover, the constructions of membrane skeleton protein‐mimicking DNA nanostructures are also highlighted.

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“…Importantly, the lipid encapsulation significantly enhances the DNA origami stability against nuclease digestion and could therefore be used as an easily accessible and non‐toxic approach to increase the bioavailability of DNA‐based nanostructures. DNA origami is a powerful tool to artificially construct and mimic cell membrane‐associated structural components, [20] and therefore, it is expected that hybrid materials assembled using DNA nanostructures and lipids will elicit multiple applications in biology and nanomedicine in the near future [19] . However, efficient strategies to assemble the hydrophobic lipids with the hydrophilic DNA origami are still needed.…”

Section: Resultsmentioning

confidence: 99%

DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies

et al. 2020

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Lipids are important building blocks in cellular compartments, and therefore their self‐assembly into well‐defined hierarchical structures has gained increasing interest. Cationic lipids and unstructured DNA can co‐assemble into highly ordered structures (lipoplexes), but potential applications of lipoplexes are still limited. Using scaffolded DNA origami nanostructures could aid in resolving these drawbacks. Here, we have complexed DNA origami together with a cationic lipid 1,2‐dioleoly‐3‐trimethylammonium‐propane (DOTAP) and studied their self‐assembly driven by electrostatic and hydrophobic interactions. The results suggest that the DNA origami function as templates for the growth of multilamellar lipid structures and that the DNA origami are embedded in the formed lipid matrix. Furthermore, the lipid encapsulation was found to significantly shield the DNA origami against nuclease digestion. The presented complexation strategy is suitable for a wide range of DNA‐based templates and could therefore find uses in construction of cell‐membrane‐associated components.

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Emerging Biomimetic Applications of DNA Nanotechnology

Cited by 51 publications

References 138 publications

Controlling aggregation of cholesterol-modified DNA nanostructures

Controlling aggregation of cholesterol-modified DNA nanostructures

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies

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