Emerging arboviruses in Rio Grande do Sul, Brazil: Chikungunya and Zika outbreaks, 2014‐2016

Gregianini, Tatiana Schäffer; Ranieri, Tani Schilling; Favreto, Cátia; Nunes, Zenaida Marion Alves; Giannini, Gabriela Luchiari Tumioto; Sanberg, Nara Druck; Rosa, Marilda Tereza Mar da; Veiga, Ana Beatriz Gorini da

doi:10.1002/rmv.1943

Cited by 35 publications

(48 citation statements)

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“…The Asian tiger mosquito, Aedes albopictus, is an aggressive and epidemiologically important insect, and its geographical range has significantly expanded over the past decades. As a public health threat, it acts as a vector for many viral agents, including those responsible for dengue fever, chikungunya and Zika fever (Hochedez et al, 2006; Correspondence: Xiao-Guang Chen Gregianini et al, 2017;Liu et al, 2017). Given that it is one of the world's 100 most invasive species, the territory of Ae.…”

Section: Introductionmentioning

confidence: 99%

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

et al. 2018

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Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit several arboviruses, including dengue, Zika and chikungunya viruses, and is considered a public health threat. Despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of Ae. albopictus has been slow due to a lack of efficient methods and genetic markers. In the present study, we established targeted disruptions in two genes, kynurenine hydroxylase (kh) and dopachrome conversion enzyme (yellow), to analyze the feasibility of generating visible phenotypes with genome editing by the clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR‐associated protein 9 (Cas9) system in Ae. albopictus. Following Cas9 single guide RNA ribonucleoprotein injection into the posterior end of pre‐blastoderm embryos, 30%–50% of fertile survivors produced alleles that failed to complement existing kh and yellow mutations. Complete eye and body pigmentation defects were readily observed in G1 pupae and adults, indicating successful generation of highly heritable mutations. We conclude that the CRISPR/Cas9‐mediated gene editing system can be used in Ae. albopictus and that it can be adopted as an efficient tool for genome‐scale analysis and biological study.

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Section: Introductionmentioning

confidence: 99%

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

et al. 2018

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“…Além dos fatores que favorecem a dispersão das doenças, o Brasil representa um país com condições ambientais ótimas para a permanência e disseminação de mosquitos vetores, como o Aedes aegypti (Gregianini et al, 2017).…”

Section: Introductionunclassified

Aedes aegypti e sociedade: o impacto econômico das arboviroses no Brasil

Teich¹,

Arinelli²,

Fahham³

2017

JBES

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RESUMOObjetivo: Arboviroses são motivo de preocupação para a saúde pública, com impactos clínicos e econômicos negativos. Este estudo teve o objetivo de avaliar custos de combate ao vetor, custos médicos diretos e custos indiretos associados à dengue clássica, dengue hemorrágica, chikungunya e infecção pelo Zika vírus (ZIKV) no Brasil, para o ano de 2016. Métodos: As análises quantificaram custos relacionados ao combate ao vetor, como repasses de recursos federais e aquisição de pesticidas, custos médicos diretos para tratamento das doenças por meio de condutas de manejo ambulatorial e hospitalar e custos indiretos relacionados ao absenteísmo, calculados com base em estudos publicados ou de estimativa de anos de vida ajustados por incapacidade (AVAIs). Resultados: O investimento para combate ao vetor foi de R$ 1,5 bilhão no Brasil e o custo reportado pelo governo federal para aquisição de inseticidas e larvicidas foi de R$ 78,6 milhões. Custos médicos diretos geraram gasto total de R$ 374 milhões. Febre chikungunya apresentou o maior número de AVAIs perdidos por episódio da doença (0,036 AVAI), seguido pela infecção por ZIKV (0,005 AVAI). O custo indireto total foi estimado em R$ 431 milhões. Custos totais com o manejo das arboviroses atingiram impacto de R$ 2,3 bilhões no Brasil, em 2016. Minas Gerais, São Paulo, Bahia e Rio de Janeiro apresentaram os maiores custos. Conclusões: Arboviroses geram consideráveis impactos econômico e social ao Brasil. Custos de combate ao vetor, custos médicos diretos e custos indiretos representaram 2% do orçamento previsto para a saúde no País, em 2016. ABSTRACTObjective: Arboviruses are a cause of concern for public health, with negative clinical and economic impacts. The objective of this study was to evaluate costs of vector control, direct medical costs and indirect costs associated with classical dengue fever, dengue hemorrhagic fever, chikungunya and Zika virus infection in Brazil for the year 2016. Methods: The analyzes quantified costs related to vector control, such as federal resource transfers and acquisition of pesticides, direct medical costs related to diseases treatment through outpatient and hospital management, and indirect costs related to absenteeism, calculated from published studies or estimates of disability-adjusted life years (DALYs). Results: The investment for vector combat was R$ 1.5 billion in Brazil and the cost reported by federal government for the acquisition of insecticides and larvicides was R$ 78.6 million. Direct medical costs generated total expenses of R$ 374 million. Fever chikungunya presented the highest number of DALYs lost per episode of disease (0.036 DALYs), followed by Zika virus infection (0.005 DALYs). Total indirect cost was estimated at R$ 431 million. Total costs with the management of arboviroses reached an impact of R$ 2.3 billion in Brazil for 2016. Minas Gerais, São Paulo, Bahia and Rio de Janeiro presented the highest costs. Conclusions: Arboviroses generate considerable economic and social impact to Brazil. Vector combat c...

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“…The first is that this virus would have gone through cospeciation with its primate host. The origin of primates from the order Platyrrhini is believed to date from approximately 30 million years ago 25 . These animals arrived in South America and expanded their distribution southwards to southern Patagonia during the Miocen Climate Optima (MCO) and retracted with the rising of the Andes and the global cooling that occurred subsequently 26 .…”

Section: Discussionmentioning

confidence: 99%

Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

Almeida

Ehlers

Demoliner

et al. 2019

Preprint

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Mosquito borne flaviviruses cause a series of important diseases in humans and animals. These viruses are maintained in cycles involving replication in mosquito and in vertebrate hosts. Most natural hosts are vertebrate animals living in sylvatic or peridomestic environments. Human contact with these environments may result in host shifts that lead to the establishment of urban transmission cycles. Zika virus is a Flavivirus that persists in nature in a transmission cycle involving non-human primates (NHP). Its recent emergence in Brazil has shed light upon the importance of surveying this agent in Brazilian sylvatic environments. Here we present histopathological and molecular evidence that free ranging howler monkeys (Alouatta guariba) in Southern Brazil are infected by ZIKV closely related to African lineage MR766. Nine NHP were nested RT-PCR positive for ZIKV RNA. Sequence analysis revealed 96 to 98% identity to ZIKV MR766 and 85% identity to ZIKV P6-740, the current epidemic strain. The affected howler monkeys presented discrete inflammatory infiltrates in several tissues and immunohistochemichal (IHC) labeling of viral antigen was observed in placenta. These findings point to the circulation of African lineage Zika virus in the Americas in non-human primates. And raises the possibility that ZIKV was introduced into the Americas on more than one occasion. 1

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Emerging arboviruses in Rio Grande do Sul, Brazil: Chikungunya and Zika outbreaks, 2014‐2016

Cited by 35 publications

References 31 publications

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

Aedes aegypti e sociedade: o impacto econômico das arboviroses no Brasil

Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

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