Emergency Use of Targeted Osmotic Lysis for the Treatment of a Patient with Aggressive Late-Stage Squamous Cell Carcinoma of the Cervix

Gould, Harry J.; Miller, Paige R.; Edenfield, Samantha; Sherman, Kelly Jean; Brady, Chad K.; Paúl, Dennis

doi:10.3390/curroncol28030196

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…These methods consist of electroporation, serving as an efficient gene delivery system [ 45 ], electrofusion [ 46 ], microinjection [ 47 ], transfection [ 48 ], liposome-mediated drug delivery [ 49 ], the fusion of cell ghost [ 50 ], cell-penetrating peptides to deliver therapeutic proteins and oligonucleotides [ 51 ], osmotic lysis [ 52 ] and laser pulses for the delivery of therapeutics into cells [ 53 ] or by glass beads [ 54 ]. As an example, mouse L cells grown in suspension culture could be rendered permeable to exogenous deoxynucleoside triphosphates by a cold shock in a slightly hypotonic buffer system containing 0.01 M Tris-HCl pH 7.8, 0.25 M sucrose, 1 mM EDTA, 30 mM 2-mercaptoethanol and 4 mM MgCl 2 [ 6 , 7 , 8 ].…”

Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

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Eukaryotic chromosomes have not been visualized during the interphase. The fact that chromosomes cannot be seen during the interphase of the cell cycle does not mean that there are no means to make them visible. This work provides visual evidence that reversible permeabilization of the cell membrane followed by the regeneration of cell membranes allows getting a glimpse behind the nuclear curtain. Reversibly permeable eukaryotic cells have been used to synthesize nascent DNA, analyze the 5′-end of RNA primers, view individual replicons and visualize interphase chromosomes. Dextran T-150 in a slightly hypotonic permeabilizing buffer prevented cells from disruption. Upon reversal of permeabilization, the nucleus could be opened at any time during the interphase. A broad spectrum of a flexible chromatin folding pattern was revealed through a series of transient geometric forms of chromosomes. Linear attachment of chromosomes was visualized in several mammalian and lower eukaryotic cells. The linear connection of chromosomes is maintained throughout the cell cycle showing that rather than individual chromosomes, a linear array of chromosomes is the functional giant macromolecule. This study proves that not only the prokaryotic genome but also linearly attached eukaryotic chromosomes form a giant macromolecular unit.

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Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

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Targeted Osmotic Lysis: A Novel Approach to Targeted Cancer Therapies

Gould

Paúl

2022

Biomedicines

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The conventional treatment of cancer has been based on the delivery of non-selective toxins and/or ionizing energy that affect both the cancer and normal tissues in the hope of destroying the offending disease before killing the patient. Unfortunately, resistance often develops to these treatments and patients experience severe, dose-limiting adverse effects that reduce treatment efficacy and compromise quality of life. Recent advances in our knowledge of the biology of tumor cells and their microenvironment, the recognition of surface proteins that are unique to specific cancers and essential to cell growth and survival and signaling pathways associate with invasion and metastasis have led to the development of targeted therapies that are able to identify specific cellular markers and more selectively deliver lethal treatment to the invading cancer thus improving efficacy and limiting adverse effects. In the context of targeted approaches to cancer therapy, we present targeted osmotic lysis as a novel and fundamentally different approach for treating advanced-stage carcinoma that exploits the conserved relationship between voltage-gated sodium channels and Na+, K+-ATPase and has the potential to increase survival without compromising quality of life in a broad spectrum of highly malignant forms of cancer.

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Cancer as a Channelopathy—Appreciation of Complimentary Pathways Provides a Different Perspective for Developing Treatments

Gould

Paúl

2022

Cancers

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Life depends upon the ability of cells to evaluate and adapt to a constantly changing environment and to maintain internal stability to allow essential biochemical reactions to occur. Ions and ion channels play a crucial role in this process and are essential for survival. Alterations in the expression of the transmembrane proteins responsible for maintaining ion balance that occur as a result of mutations in the genetic code or in response to iatrogenically induced changes in the extracellular environment is a characteristic feature of oncogenesis and identifies cancer as one of a constellation of diseases known as channelopathies. The classification of cancer as a channelopathy provides a different perspective for viewing the disease. Potentially, it may expand opportunities for developing novel ways to affect or reverse the deleterious changes that underlie establishing and sustaining disease and developing tolerance to therapeutic attempts at treatment. The role of ions and ion channels and their interactions in the cell’s ability to maintain ionic balance, homeostasis, and survival are reviewed and possible approaches that mitigate gain or loss of ion channel function to contribute to new or enhance existing cancer therapies are discussed.

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Emergency Use of Targeted Osmotic Lysis for the Treatment of a Patient with Aggressive Late-Stage Squamous Cell Carcinoma of the Cervix

Cited by 3 publications

References 23 publications

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Targeted Osmotic Lysis: A Novel Approach to Targeted Cancer Therapies

Cancer as a Channelopathy—Appreciation of Complimentary Pathways Provides a Different Perspective for Developing Treatments

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