Emergence of microfluidic devices in sample extraction; an overview of diverse methodologies, principals, and recent advancements

Alidoust, Mina; Baharfar, Mahroo; Manouchehri, Mahshid; Yamini, Yadollah; Tajik, Mohammad; Seidi, Shahram

doi:10.1016/j.trac.2021.116352

Cited by 27 publications

(10 citation statements)

References 179 publications

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“…We also foresee such a sensor being part of a fieldable or point of care device. Such devices are being developed with preconcentration modules for direct testing of samples, which could help focus on a separate or initial protease evaluation. , Alternatively or simultaneously if desired, a sample could be extracted and preconcentrated by a module on the device and then undergo multitarget amplification by PCR or another isothermal process where the current sensor would be utilized for multiplexed screening and detection . In terms of optical detection, we note the recent development of a portable 3D smartphone-based device utilizing a double-chopper design for TG PL assay imaging .…”

Section: Discussionmentioning

confidence: 99%

Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor

Hastman,

Hooe,

Chiriboga

et al. 2023

ACS Sens.

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Almost all pathogens, whether viral or bacterial, utilize key proteolytic steps in their pathogenesis. The ability to detect a pathogen's genomic material along with its proteolytic activity represents one approach to identifying the pathogen and providing initial evidence of its viability. Here, we report on a prototype biosensor design assembled around a single semiconductor quantum dot (QD) scaffold that is capable of detecting both nucleic acid sequences and proteolytic activity by using orthogonal energy transfer (ET) processes. The sensor consists of a central QD assembled via peptidyl-PNA linkers with multiple DNA sequences that encode complements to genomic sequences originating from the Ebola, Influenza, and COVID-19 viruses, which we use as surrogate targets. These are hybridized to complement strands labeled with a terbium (Tb) chelate, AlexaFluor647 (AF647), and Cy5.5 dyes, giving rise to two potential FRET cascades: the first includes Tb → QD → AF647 → Cy5.5 (→ = ET step), which is detected in a time-gated modality, and QD → AF647 → Cy5.5, which is detected from direct excitation. The labeled DNAdisplaying QD construct is then further assembled with a Ru II -modified peptide, which quenches QD photoluminescence by charge transfer and is recognized by a protease to yield the full biosensor. Each of the labeled DNAs and peptides can be ratiometrically assembled to the QD in a controllable manner to tune each of the ET pathways. Addition of a given target DNA displaces its labeled complement on the QD, disrupting that FRET channel, while protease addition disrupts charge transfer quenching of the central QD scaffold and boosts its photoluminescence and FRET relay capabilities. Along with characterizing the ET pathways and verifying biosensing in both individual and multiplexed formats, we also demonstrate the ability of this construct to function in molecular logic and perform Boolean operations; this highlights the construct's ability to discriminate and transduce signals between different inputs or pathogens. The potential application space for such a sensor device is discussed.

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Section: Discussionmentioning

confidence: 99%

Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor

Hastman,

Hooe,

Chiriboga

et al. 2023

ACS Sens.

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“…Liquid–liquid extraction plays an important role in industrial applications. To improve the mass-transfer efficiency, miniaturized devices have been integrated with liquid–liquid extraction processes especially in biochemical processes, , pharmaceutical production, − and analytical chemistry, , for it could provide shorter mass-transfer lengths, larger interface areas, and low energy/material consumption. In 1996, Liu and Dasgupta developed a liquid-phase microextraction (LPME) system.…”

Section: Introductionmentioning

confidence: 99%

Continuous Counter-Current Multistage Microextraction in a Novel Tube-In-Tube Microextractor

Chen

Zhou

Xie

et al. 2023

Ind. Eng. Chem. Res.

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Counter-current operation has been widely applied to enhance the extraction efficiency for systems with low distribution coefficients, which was hindered in microextraction because viscous force and surface tension dominate over gravity and inertial force. In this study, a novel design of a tube-in-tube microextractor relying on a poly tetra fluoroethylene membrane to construct a stable interface was proposed to conduct continuous counter-current extraction. Extraction kinetics for two systems with different distribution coefficients, membranes with different pore radii, and three flow rate ratios were measured as a function of residence time. For the system that toluene was used to extract 0.06 wt % phenol in water, 4.1 stages of extraction efficiency were achieved at a residence time of 9.8 min. A challenging case study (50 wt % acetone−water−toluene) was subsequently conducted to prove the high applicability for emulsion systems of this special microextractor. Lastly, a mathematical model was established and the predicted results of three flow rate ratios are in good agreement with experimental results.

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“…Microfluidic devices have undergone significant improvements over the past decade, with advances in fabrication techniques, materials, integration, and applications [1]. These improvements have enabled the development of more advanced and sophisticated microfluidic devices, which are being used in a wide range of applications, including drug delivery [2], diagnostics [3], environmental monitoring [4,5], and biological sample analysis [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

An improved microfluidic device to enhance the enrichment factors in liquid phase microextraction: application to the simultaneous extraction of polar and non-polar acids in biological samples

et al. 2023

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A new microfluidic device to enhance the enrichment factor in miniaturized systems is proposed. The microfluidic system was design for liquid phase microextractions, and it was applied to the simultaneous extraction of acidic compounds of a wide range of polarity (0.5 < log P < 3). The device operated under stagnant acceptor phase conditions and all the operational parameters involved were optimized. Tributyl phosphate was found to be a new highly efficient supported liquid membrane to simultaneously extract analytes of very different polarities. The optimal donor and acceptor phase were pH 2 and pH 13, respectively. The donor flow rate and the extraction time were investigated simultaneously, offering great versatility with high enrichment factors (EFs). Limits of quantitation were within 0.02 and 0.09 µg mL−1 for all compounds at 10 µL min−1 as donor flow rate and 20-min extractions, offering EFs between 11 and 18 with only 200-µL sample volume consumption. The method was successfully applied to human urine samples, observing recoveries between 47 and 90% for all compounds. This new proposed microfluidic system increases the wide range of applications, especially when the analytes are present in lower concentrations in the sample. Graphical Abstract

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Emergence of microfluidic devices in sample extraction; an overview of diverse methodologies, principals, and recent advancements

Cited by 27 publications

References 179 publications

Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor

Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor

Continuous Counter-Current Multistage Microextraction in a Novel Tube-In-Tube Microextractor

An improved microfluidic device to enhance the enrichment factors in liquid phase microextraction: application to the simultaneous extraction of polar and non-polar acids in biological samples

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