Emergence of Lumpy Skin Disease in Greece, 2015

Tasioudi, Konstantia E.; Antoniou, Sotiria‐Eleni; Iliadou, Peristera; Sachpatzidis, Achilleas; Plevraki, E.; Agianniotaki, Eirini I.; Fouki, Christina; Mangana-Vougiouka, O; Chondrokouki, Eleni; Dile, C.

doi:10.1111/tbed.12497

Cited by 117 publications

(105 citation statements)

References 12 publications

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“…Vaccine lots 444 and 446 were used. The immunizations were performed within the framework of the emergency LSD vaccination which is being implemented in Greece from the beginning of the 2015 epizootic (Tasioudi et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, from August 2015, and until the end of December 2015, 117 outbreaks have been laboratory‐confirmed in six regional units of Northern Greece (Central Macedonia, Eastern Macedonia and Thrace) and the Island of Limnos. Approximately 6,000 cattle were killed, within the framework of the stamping‐out policy (Tasioudi et al., ). By December 2015, the spread of LSD in Greece stopped.…”

Section: Introductionmentioning

confidence: 99%

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Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Katsoulos

Chaintoutis

Dovas

et al. 2017

Transbound Emerg Dis

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SummaryThe present study was performed to investigate the clinical impact and certain virological and haematological parameters following immunization of cattle against lumpy skin disease (LSD). The study was conducted in a dairy cattle farm (215 animals), immunized with a Neethling strain-based live vaccine. Twenty-seven animals (14 lactating cows, four dry cows and nine calves) were randomly selected for repetitive blood and saliva samplings. An EvaGreen-based real-time PCR was designed to differentiate vaccine from field LSDVs. Vaccinated animals underwent examination for adverse reactions. Nodule samples were collected from two representative cases for histopathological testing and virus identification. Milk yield was calculated based on bulk-tank measurements of all lactating cows (79). Viral DNA was detected between days 6-15 post-vaccination (p.v.) at 63% of the sampled animals (17/27). Saliva and bulk-tank milk samples were LSDV-negative. Pronounced swelling was observed at injection sites of 12% of the immunized animals (26/215), starting at day 6 p.v., and was resolved after 2-4 days. Small-sized (<0.5 cm) cutaneous lumps were developed between days 8-18 p.v. at 9% of the vaccinated animals (19/215). These were observed in adult cows and not in calves/heifers.Resolution was observable 10 days post-development. The vaccine virus was also identified in nodules and injection-site aspirates. Haematological changes (e.g., lower leucocyte counts) were observed in cows and not in calves. Daily milk production was being reduced during the first 12 days p.v. LSD immunization of cows resulted in nodules and low viraemia levels. The fact that nodules and haematological changes were not observed in calves, along with the low viraemia, supports the reduced virulence of the Neethling vaccine strain. The characteristic nodules in vaccinated animals could allow clinical differentiation from those observed in LSD. The developed real-time PCR efficiently differentiates infected from vaccinated cattle, and should be further validated as a tool in LSD surveillance. † The first two authors contributed equally to this work.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Katsoulos

Chaintoutis

Dovas

et al. 2017

Transbound Emerg Dis

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“…It is feared that the disease could cross boundaries into neighbouring countries or spread rapidly throughout the region and become a pandemic. Recently, the disease was reported in Greece in 2015 (18). It is likely that this disease will spread to the rest of Europe.…”

Section: Resultsmentioning

confidence: 99%

“…The disease is endemic in many African countries. Since 2000, it has spread to the Middle East, was confirmed in Turkey in 2013, and is now prevalent in many countries in the European Union (9,18,20).…”

Section: Praca Oryginalnamentioning

confidence: 99%

Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey

Albayrak¹,

Özan²,

Kadı³

et al. 2018

Medycyna Weterynaryjna

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The aim of this study was to investigate the prevalence and distribution patterns of LSDV infections in the provinces of northern Turkey, and to detect the factors influencing the epidemiology of LSD virus infections (age, breed, season, climate, geography, population dynamic, animal movement), as well as to assess the diagnostic value of the sampled materials in the diagnosis of LSDV infections. Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Turkey and the Middle East. In this study, a total of 564 samples (skin, blood and lung) from different cattle breeds (Jersey, Holstein-Friesian, Anatolian Black, Simmental and Brown Swiss) (n=465) in the many herds suspected of lumpy skin disease virus (LSDV) infection as clinically and macroscopic pathologic remarks, housed in the 7 different provinces of northern Turkey, were used for gel based conventional polymerase chain reaction (PCR). LSDV nucleic acid was detected in 259 of 564 (45.92%) materials by PCR. According to the result of PCR, the LSDV infection was diagnosed in 54.62% (254/465) of the sampled animals. The diagnostic value of necropsy and clinical materials such as skin and lung were determined as more valuable diagnostic materials in the diagnosis of LSDV infection by PCR. Data showed that LSDV infection was widespread in the provinces of northern Turkey and that the prevalence of the infection in the region varies in accordance with factors such as geographical conditions (climate, season, location etc.) and the method of breeding. .

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“…Historically restricted to Africa, the disease spread to the Middle East in 2012 and Turkey in 2013 (1). In August 2015, the first LSD cases in mainland Europe were observed in Greece in the Evros region at the European border with Turkey (2). The disease has been spreading into Europe ever since (3, 4).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

et al. 2017

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Emergence of Lumpy Skin Disease in Greece, 2015

Cited by 117 publications

References 12 publications

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey

Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

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