Emergence of KPC-2-Producing Pseudomonas aeruginosa Sequence Type 463 Isolates in Hangzhou, China

Abstract

Thirty-nine Klebsiella pneumoniae carbapenemase (KPC)-producing Pseudomonas aeruginosa isolates, all exhibiting high-level resistance to carbapenems and other ␤-lactam antibiotics, were isolated in Hangzhou, China. Molecular epidemiology analysis indicated the presence of two dominant clones, namely, clones A and B, both of which belong to sequence type 463 (ST463). A genetic environment analysis demonstrated that both clones harbor an ISKpn8 transposase, bla KPC-2 , and an ISKpn6-like transposase. These findings depict the features of clonal expansion and transmission of KPC-2-producing P. aeruginosa strains in Hangzhou, China.

Emergence of Raoultella ornithinolytica Coproducing IMP-4 and KPC-2 Carbapenemases in China

“…The bla KPC-2 gene in R. ornithinolytica is also located on a Tn3-Tn4401 integration structure, as previously described from K. pneumoniae and E. coli (8,20). Of note, the bla KPC-2 gene has been detected in K. pneumoniae, E. coli, Enterobacter cloacae, Morganella morganii, and even Pseudomonas aeruginosa isolates from Zhejiang province (16,(21)(22)(23). Our observation further documents the transmission of this structure in different Enterobacteriaceae in China.…”

Epidemiological surveillance of multidrug‐resistant gram‐negative bacteria in a solid organ transplantation department

“…In Greece, as in most other countries, the problem of resistance mainly concerns gram‐negative bacteria, namely Klebsiella pneumoniae , Pseudomonas aeruginosa , and Acinetobacter baumannii . These bacteria can acquire resistance, even multidrug resistance (MDR), through various mechanisms and transmit this resistance . Indeed, cross‐transmission of the resistant strains between patients is of great importance and determined by several factors, mainly related to hospitalization conditions and implementation of measures to prevent infections …”

Global Dissemination of bla KPC into Bacterial Species beyond Klebsiella pneumoniae and In Vitro Susceptibility to Ceftazidime-Avibactam and Aztreonam-Avibactam

“…The impact of KPC became more fully recognized as this family of enzymes became a global threat to public health, in that the gene encoding KPC (bla KPC ) has now been observed in multiple Enterobacteriaceae species and has disseminated worldwide, in large part due to the spread of K. pneumoniae isolates belonging to the successful high-risk clonal complex 258 (7,13). bla KPC is most often embedded within the Tn4401 transposon, though it has also been reported in other mobile elements, and found in plasmids belonging to 12 incompatibility groups capable of species-to-species transfer within Enterobacteriaceae and some nonfermentative Gram-negative pathogens, including Pseudomonas aeruginosa (14)(15)(16)(17). Furthermore, these plasmids commonly also carry genes encoding aminoglycoside resistance mechanisms and additional ␤-lactamases, including extended-spectrum ␤-lactamases (ESBLs) (12,17).…”

“…bla KPC is most often embedded within the Tn4401 transposon, though it has also been reported in other mobile elements, and found in plasmids belonging to 12 incompatibility groups capable of species-to-species transfer within Enterobacteriaceae and some nonfermentative Gram-negative pathogens, including Pseudomonas aeruginosa (14-17). Furthermore, these plasmids commonly also carry genes encoding aminoglycoside resistance mechanisms and additional ␤-lactamases, including extended-spectrum ␤-lactamases (ESBLs) (12,17). bla KPC has also been found inserted into the bacterial chromosome (18,19).…”