Emergence of foot and mouth disease virus, Lib‐12 lineage of topotype
            <scp>VII</scp>
            , serotype
            <scp>SAT</scp>
            2 in Egypt, 2018

Soltan, Mohamed A.; Dohreig, Ragab M. A.; Abbas, Hussein A.; Ellawa, Mohamed; Yousif, Ibrahim A.; Aly, Ayman E.; Wasfy, Momtaz; El-Sayed, M. M.

doi:10.1111/tbed.13152

Cited by 13 publications

(15 citation statements)

References 6 publications

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“…The results of conventional RT-PCR assays in this study (Table 5), indicated that FMDV SAT2 was detected in Egypt during 2018-2020. The most prevalent serotype responsible for the outbreaks is SAT2 and this is in alignment with the findings reported earlier (Ahmed et al 2012;FAO 2012;Kandeil et al 2013;ElShehawy et al 2014;Soltan et al 2018). Furthermore, the low detection rate of serotypes A and O is supported by (Kandeil et al 2013), who stated that the low incidence of serotypes A and O may be due to regular vaccination control in Egypt during the period of study.…”

Section: Discussionsupporting

confidence: 84%

“…The obtained VP1 nucleotide sequence of serotype SAT2 Sharkia 2018 and 2019 strains were compared as shown in (Fig. 6 to 10) with other isolates in the database indicated that the detected strain is closely related to SAT-2/LIB/41/2012, SAT-2, Ismailia/1.2.3/2018 which belong to topotype VII and other Egyptian isolates published during 2018, these results in alignment with the findings of Soltan et al (2019) who expected that Egyptian outbreaks of FMDV SAT2 during the period of 2018 -2019 may be originated from Libya. Also, these results in close agreement with the findings of Hagag et al (2019) who detected that FMD SAT2 circulated in Egypt in 2018-2019 and reported reemergence of FMD serotype O Manisa in Egyptian cattle in 2019.…”

Section: Discussionsupporting

confidence: 81%

“…Egyptian veterinary authorities started an emergency vaccination campaigns using the regularly used trivalent vaccines of serotype O, topotype ME-SA, Panasia2 lineage; serotype A, Asian topotype, Iran-05 lineage; and serotype SAT2, topotype VII, Gharbia12 lineage) and locally produced univalent vaccine containing exotic strain (Serotype SAT2, topotype VII, Lib12 lineage) as described by Soltan et al (2019) manufactured by the Middle East for Veterinary Vaccines (MEVAC, Egypt).…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Molecular Characterization of Circulating Foot and Mouth Disease Virus in Egypt During 2018-2020

2021

Int J Vet Sci

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Foot and Mouth Disease (FMD) is a contagious viral disease with high economic losses and primary animal health concerns. FMDV type SAT2 is endemic in Egypt since 2012. This work aimed to characterize the circulating FMDV SAT2 strains genetically in Egypt from 2018 to 2020. A total of 209 vesicular fluids and tongue epithelium were collected from infected cattle and buffaloes in Sharkia, Ismailia, and Dakhlia provinces. All samples were examined by real-time PCR and conventional PCR for FMDV using pan- serotype and serotype-specific primers targeting the VP1 region. Out of 209 samples, 45 infected animals were positive for FMDV SAT2 virus, 29 cattle (21.5%), and 16 buffaloes (13.6%). No FMDV serotype A or O were detected. The highest prevalence of FMDV SAT2 was observed in Sharkia province with a percentage of 10% followed by Ismailia and Dakhlia with a rate of 2.9 and 0.9%, respectively. Three FMDV SAT2 positive samples represented as Sharkia 2018 and Sharkia 2019 and Ismailia 2020 were selected for sequencing and phylogenetic analysis of VP1. Sequencing and phylogenetic analysis of VP1of the three Egyptian strains demonstrated that these strains are closely related to other Egyptian strains in gene bank as Alex 2018 (MK4933346), Ismailia 2018 (MK4933341), and Menofia 2018 (MT199283) with homology ranged from 95.8 to 98.2%. Phylogenetic tree of FMDV SAT2 showed clustering of Sharkia 2018, Sharkia 2019, and Ismailia 2020 with Libya 2012 topotype VII with three amino acid substitutions at the site 24, 28, and 52.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Isolation and Molecular Characterization of Circulating Foot and Mouth Disease Virus in Egypt During 2018-2020

2021

Int J Vet Sci

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“…The majority of SAT 2 viruses (n = 21) were characterized as belonging to the XIII topotype, which was first reported from a single isolate collected in 2007 (Ayelet et al, 2009), suggesting possible virus introduction(s) from Sudan and subsequent persistence in Ethiopia at least through 2010 ( Figure S4). The other SAT 2 viruses from topotype VII, Alx-12 and Lib-12 lineages, were found to be closely related to viruses collected across several countries of East, West and North Africa ( Figure S4) (Ehizibolo et al, 2017;Soltan et al, 2019).…”

Section: Discussionmentioning

confidence: 85%

Molecular characterization of foot‐and‐mouth disease viruses circulating in Ethiopia between 2008 and 2019

Gizaw¹,

Tesfaye

Wood

et al. 2020

Transbound. Emerg. Dis.

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“…Since 2012, livestock in Egypt have suffered occasional FMD outbreaks due to different FMDV strains, resulting in significant losses among calves [8,9]; moreover, even co-infections with other infectious pathogens have been reported [10]. The appearance of new lineages from the different serotypes is associated with increased mortality rates in young ruminants and adults, inflicting severe economic losses [11,12]. All FMDV serotypes produce a clinically indistinguishable disease, but immunity to one serotype does not protect against the others due to the wide antigenic diversity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Foot and Mouth Disease Virus Serotype SAT-2 in Swamp Water Buffaloes (Bubalus bubalis) under the Egyptian Smallholder Production System

Damaty

Fawzi

Neamat‐Allah

et al. 2021

Animals

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Spontaneous mutations are a common characteristic of the foot and mouth disease virus (FMDV), leading to wide antigenic variations resulting in the emergence of new topotypes and lineages of FMDV, which contributes to occasional vaccination failures. The objectives of the present study were to genetically characterize FMDV isolated from water buffaloes and study the biochemical and histopathological indicators of infected animals. Fifty-four water buffaloes of both sexes and different ages suffered from acute symptoms of FMD were clinically examined and randomly selected for inclusion in this study. Oral desquamated epithelial and oropharyngeal fluid samples have been tested for FMDV by reverse transcriptase PCR (RT-PCR). Tissue and serum samples were also collected from the diseased buffaloes and subjected to histopathological and biochemical analysis. Our findings showed that all examined samples were confirmed to be positive to FMDV serotype SAT-2 and were adjusted to be responsible for the recent disease outbreak in this study. Phylogenetic analysis revealed that the circulating viruses were of the SAT-2 serotype, closely related to the lineage of lib12, topotype VII, with 98.9% identity. The new lineage of SAT-2 showed a high virulence resulting in the deaths of water buffaloes due to heart failure, confirmed by high serum levels of inflammatory and cardiac markers, including haptoglobin, ceruloplasmin, cardiac troponin I and creatine phosphokinase-MB, indicating an unfavorable FMD-infection prognosis. In conclusion, we document the presence of new incursions circulating in water buffalo populations in Egypt in early 2019, explaining the high morbidity rate of FMD outbreak in early 2019. Furthermore, the newly identified serotype SAT-2 lib12 lineage, topotype VII, showed an aggressive pattern in water buffaloes of the smallholder production system.

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Emergence of foot and mouth disease virus, Lib‐12 lineage of topotype VII , serotype SAT 2 in Egypt, 2018

Cited by 13 publications

References 6 publications

Isolation and Molecular Characterization of Circulating Foot and Mouth Disease Virus in Egypt During 2018-2020

Isolation and Molecular Characterization of Circulating Foot and Mouth Disease Virus in Egypt During 2018-2020

Molecular characterization of foot‐and‐mouth disease viruses circulating in Ethiopia between 2008 and 2019

Characterization of Foot and Mouth Disease Virus Serotype SAT-2 in Swamp Water Buffaloes (Bubalus bubalis) under the Egyptian Smallholder Production System

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