Emergence of Extensively Drug-Resistant ST170 Citrobacter portucalensis with Plasmids pK218-KPC, pK218-NDM, and pK218-SHV from a Tertiary Hospital, China

Luo, Xinhua; Yu, Lei; Feng, Jiao; Zhang, Jin; Zheng, Cheng; Hu, Dakang; Dai, Piaopiao; Xu, Mengqiao; Li, Piaopiao; Lin, Ran; Mu, Kai

doi:10.1128/spectrum.02510-22

Cited by 6 publications

(7 citation statements)

References 42 publications

(45 reference statements)

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“…However, the misidentification of C. portucalensis as C. freundii has led to an underestimation of its prevalence in clinical settings. 7,26 In this study, L2724hy C. portucalensis was initially misidentified as C. freundii using MALDI-TOF/MS, and was later identified as C. portucalensis by a combination of 16s rRNA sequencing and ANI analysis. Thus, it is essential to enhance the whole-genome sequencing of less common strains.…”

Section: Discussionmentioning

confidence: 97%

“…2 Subsequently, in 2022, the coexistence of bla KPC-2 and bla NDM-1 was reported in an extensively drug-resistant C. portucalensis obtained from a hospital in China. 7 Additionally, in 2023, researchers isolated C. portucalensis carrying bla NDM-1 from an endangered marine animal that died from sepsis. 8 This growing body of evidence underscores the increasing clinical and extra-clinical dissemination of drug-resistant C. portucalensis, warranting heightened attention to address the associated public health concerns.…”

Section: Introductionmentioning

confidence: 99%

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Emergence of an Extensive Drug Resistant Citrobacter portucalensis Clinical Strain Harboring blaSFO-1, blaKPC-2, and blaNDM-1

Guo,

Zhao,

Yang

et al. 2024

IDR

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Emergence of an Extensive Drug Resistant Citrobacter portucalensis Clinical Strain Harboring blaSFO-1, blaKPC-2, and blaNDM-1

Guo,

Zhao,

Yang

et al. 2024

IDR

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“…A number of studies reported that few ANT(3") enzymes, encoded by aadA (aadA1, aadA2, aadA16), conferred resistance to spectinomycin and streptomycin in Citrobacter spp. (Babiker et al, 2020;Ramsamy et al, 2020;Moser et al, 2021;Han et al, 2022;Luo et al, 2022). The presence of streptomycin inactivating genes (strA, strB) was also reported in Citrobacter spp.…”

Section: Antibiotic Resistancementioning

confidence: 95%

“…The presence of streptomycin inactivating genes (strA, strB) was also reported in Citrobacter spp. (Yao et al, 2021;Luo et al, 2022).…”

Section: Antibiotic Resistancementioning

confidence: 99%

A brief insight into Citrobacter species - a growing threat to public health

Jabeen,

Islam,

Hassan

et al. 2023

Front. Antibiot.

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Citrobacter spp. are Gram-negative, non-spore forming, rod-shaped, facultative anaerobic bacteria from the Enterobacteriaceae family often found in soil, sewage, sludge, water, food, and the intestinal tracts of animals and humans. Several members of Citrobacter spp. especially C. freundii, C. koseri, C. braakii are frequently detected in newborn illnesses, urinary tract infections, and patients with severe underlying conditions, including hypertension, diabetes, cancer, and respiratory infections, or those who are immunocompromised. Strains of Citrobacter spp. can spread vertically or horizontally from carriers or other hospital sources and thus cause nosocomial infections in hospital settings. A total of 19 Citrobacter genomospecies have been recognized based on genomics. It has been noted that the Citrobacter genus acquired antimicrobial resistance and virulence, including invasion, colonization, biofilm formation, and toxin production. The recent emergence and spread of antimicrobial resistance to β-lactams, carbapenems, fluoroquinolones, aminoglycosides, and colistin in Citrobacter spp. through chromosomal and plasmid-mediated resistance limits the empiric treatment options. Therefore, combination therapy involving costly and potentially hazardous antibiotics poses significant challenges in treating Citrobacter infections. Here we summarized the nomenclature of Citrobacter spp., clinical manifestations, epidemiology, pathogenesis, antibiotic resistance mechanisms, and treatments from various clinical samples. This review will expand our knowledge of the genomics and epidemiology of Citrobacter spp., enabling improved control of infections and the spread of these organisms.

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“…The donor and recipient strains were grown in 3 mL LB broth overnight at 37°C. Subsequently, 50 μL of donor strain culture was mixed with 500 μL of recipient strain culture (v:v = 1:10) and 4.5 mL of fresh LB broth (Luo et al, 2022). In addition, 100 μL of the mixture was applied onto a cellulose filter membrane (pore size, 0.22 μm) already placed on an LB agar plate.…”

Section: Conjugation Experimentsmentioning

confidence: 99%

Convergence of plasmid-mediated Colistin and Tigecycline resistance in Klebsiella pneumoniae

Zhao,

Qian,

et al. 2024

Front. Microbiol.

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ObjectiveThe co-occurrence of colistin and tigecycline resistance genes in Klebsiella pneumoniae poses a serious public health problem. This study aimed to characterize a K. pneumoniae strain, K82, co-harboring a colistin resistance gene (CoRG) and tigecycline resistance gene (TRG), and, importantly, investigate the genetic characteristics of the plasmid with CoRG or TRG in GenBank.MethodsK. pneumoniae strain K82 was subjected to antimicrobial susceptibility testing, conjugation assay, and whole-genome sequencing (WGS). In addition, comparative genomic analysis of CoRG or TRG-harboring plasmids from K82 and GenBank was conducted. K. pneumoniae strain K82 was resistant to all the tested antimicrobials including colistin and tigecycline, except for carbapenems.ResultsWGS and bioinformatic analysis showed that K82 belonged to the ST656 sequence type and carried multiple drug resistance genes, including mcr-1 and tmexCD1-toprJ1, which located on IncFIA/IncHI2/IncHI2A/IncN/IncR-type plasmid pK82-mcr-1 and IncFIB/IncFII-type plasmid pK82-tmexCD-toprJ, respectively. The pK82-mcr-1 plasmid was capable of conjugation. Analysis of the CoRG/TRG-harboring plasmid showed that mcr-8 and tmexCD1-toprJ1 were the most common CoRG and TRG of Klebsiella spp., respectively. These TRG/CoRG-harboring plasmids could be divided into two categories based on mash distance. Moreover, we found an IncFIB/IncHI1B-type plasmid, pSYCC1_tmex_287k, co-harboring mcr-1 and tmexCD1-toprJ1. To the best of our knowledge, this is the first report on the co-occurrence of mcr-1 and tmexCD1-toprJ1 on a single plasmid.ConclusionOur research expands the known diversity of CoRG and TRG-harboring plasmids in K. pneumoniae. Effective surveillance should be implemented to assess the prevalence of co-harboring CoRG and TRG in a single K. pneumoniae isolate or even a single plasmid.

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Emergence of Extensively Drug-Resistant ST170 Citrobacter portucalensis with Plasmids pK218-KPC, pK218-NDM, and pK218-SHV from a Tertiary Hospital, China

Cited by 6 publications

References 42 publications

Emergence of an Extensive Drug Resistant Citrobacter portucalensis Clinical Strain Harboring blaSFO-1, blaKPC-2, and blaNDM-1

Emergence of an Extensive Drug Resistant Citrobacter portucalensis Clinical Strain Harboring blaSFO-1, blaKPC-2, and blaNDM-1

A brief insight into Citrobacter species - a growing threat to public health

Convergence of plasmid-mediated Colistin and Tigecycline resistance in Klebsiella pneumoniae

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