Emergence of clinical isolates of Pseudomonas asiatica and Pseudomonas monteilii from Japan harbouring an acquired gene encoding a carbapenemase VIM-2

Abstract

Pseudomonas asiatica and Pseudomonas monteilii , belonging to the Pseudomonas putida phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as P. putida in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of P. asiatica and P. monteilii were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying bla VIM-2 was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of P. asiatica (two isolates) and P. monteilii (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured blaVIM-2 . The two isolates of P. asiatica had a different pattern in the PFGE analysis, with both having a 23 kb plasmid. Of the four isolates of P. monteilii with similar patterns in the PFGE analysis, three had 320 kb plasmids and one had a 240 kb plasmid. The genetic environments of the 320/240 kb and 23 kb plasmids differed. The results strongly indicated that carbapenem-resistant P. asiatica and P. monteilii producing metallo-β-lactamase are emerging in Japan. This is the first report of carbapenem-resistant P. asiatica and P. monteilii in Japan.

Genomic Epidemiology of MBL-Producing Pseudomonas putida Group Isolates in Poland

“…The study revealed predominance of P. alloputida, P. monteilii and P. asiatica in the entire group (* 76% in total), which in general corresponds to other reports [5,6,18,51] and numbers of genomic sequences available at the time of the study. The clonality and phylogenetic analyses revealed the presence of three lineages, one per each of the major species, of the apparently broad geographic distribution, which may be considered to be epidemiologically 'successful' clones.…”

“…In several crosssectional population studies, it has been suggested that the P. putida group may act as a significant AMR reservoir [4,11,13,16], however, not all authors have shared this opinion [17]. Out of 51 species currently included in the P. putida group, a small portion have only been repeatedly identified in nosocomial infections, namely P. alloputida, P. monteilii, P. asiatica, P. kurunegalensis, and P. juntendi [4,6,18]. For the majority of those, the broader population clonal structures and potentially epidemic genotypes remain almost unexplored [19].…”

Genomic Analysis of Pseudomonas asiatica JP233: An Efficient Phosphate-Solubilizing Bacterium

“…P. asiatica was first described in 2019 [ 49 ], and has been isolated from water [ 50 ], soil [ 51 ], and human stool samples [ 49 ]. Strains of this species are functionally diverse and have been reported to be resistant to antimicrobials and heavy metals [ 50 , 52 ] and are capable of degrading the generally recalcitrant advanced glycation end products Nε-carboxymethyllysine and Nε–carboxyethyllysine [ 51 ]. P. asiatica C1 has been reported to aerobically synthesize coenzyme B 12 and was developed as a microbial cell factory for the synthesis of 3-hydroxypropionic acid from glycerol [ 53 ].…”

“…Heavy metal tolerant bacteria have been used in bioremediation processes to improve plant growth in soils contaminated with heavy metals [ 72 ]. P. asiatica strains have previously been reported to be highly tolerant to heavy metals [ 50 , 52 ]. The present genome analysis of P. asiatica JP233 identified genes encoding tolerance to arsenic ( arsH , arsC , arsB , arsR ), copper ( cusR , cusS , copB ), chromium ( chrA and chrR ), zinc ( zntA ) and cadmium ( cadR ).…”

Pseudomonas paralcaligenes sp. nov., isolated from a hospitalized patient

“… Pseudomonas is a complex genus of Gram-stain-negative species [1], with 259 species currently listed in the List of Prokaryotic names with Standing in Nomenclature (LPSN) (http://www.bacterio.net) [2]. Many species of the genus Pseudomonas can cause opportunistic infections in humans, including Pseudomonas aeruginosa, Pseudomonas alcaligenes [3], Pseudomonas asiatica [4], Pseudomonas fluorescens [5], Pseudomonas monteilii [6], Pseudomonas putida [7] and Pseudomonas stutzeri [7].…”