Genomic Analysis of Pseudomonas asiatica JP233: An Efficient Phosphate-Solubilizing Bacterium

Abstract

The bacterium Pseudomonas sp. strain JP233 has been reported to efficiently solubilize sparingly soluble inorganic phosphate, promote plant growth and significantly reduce phosphorus (P) leaching loss from soil. The production of 2-keto gluconic acid (2KGA) by strain JP233 was identified as the main active metabolite responsible for phosphate solubilization. However, the genetic basis of phosphate solubilization and plant-growth promotion remained unclear. As a result, the genome of JP233 was sequenced and analyzed in this study. The JP233 genome consists of a circular chromosome with a size of 5,617,746 bp and a GC content of 62.86%. No plasmids were detected in the genome. There were 5097 protein-coding sequences (CDSs) predicted in the genome. Phylogenetic analyses based on genomes of related Pseudomonas spp. identified strain JP233 as Pseudomonas asiatica. Comparative pangenomic analysis among 9 P. asiatica strains identified 4080 core gene clusters and 111 singleton genes present only in JP233. Genes associated with 2KGA production detected in strain JP233, included those encoding glucose dehydrogenase, pyrroloquinoline quinone and gluoconate dehydrogenase. Genes associated with mechanisms of plant-growth promotion and nutrient acquisition detected in JP233 included those involved in IAA biosynthesis, ethylene catabolism and siderophore production. Numerous genes associated with other properties beneficial to plant growth were also detected in JP233, included those involved in production of acetoin, 2,3-butanediol, trehalose, and resistance to heavy metals. This study provides the genetic basis to elucidate the plant-growth promoting and bio-remediation properties of strain JP233 and its potential applications in agriculture and industry.

Soil phosphorus transformation and plant uptake driven by phosphate-solubilizing microorganisms

“…Glucose can form gluconic acid through the synergistic effects of pyrroloquinoline quinine (PQQ) and glucose dehydrogenase (GDH), which dissolves insoluble phosphate ( Jaiswal et al, 2021 ). Pseudomonas produces gluconic acid to increase phosphate solubility, which has become an important technology for improving phosphate fertilizer management in modern agriculture ( Wang et al, 2022 ; Rai et al, 2023 ). Inoculation of Pseudomonas fluorescens and Pseudomonas putida under soluble phosphate-restricted conditions can produce a large amount of gluconic acid, which promotes plant growth ( Jin et al, 2022 ).…”

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

“…S119, a gene coding the biosynthesis pathway of gluconic acid. It is also a major member of the group that dissolves insoluble phosphorus in soil [148,149]. Some studies have also found an interaction between AFM and PSB.…”