Emergence of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> Clinical Isolates Collected from Some Libyan Hospitals

Mathlouthi, Najla; Areig, Zaynab; Al-Bayssari, Charbel; Bakour, Sofiane; Salabi, Allaaeddin Ali El; Gwierif, Salha Ben; Zorgani, Abdulaziz; Slama, Karim Ben; Chouchani, Chedly; Rolain, Jean‐Marc

doi:10.1089/mdr.2014.0235

Cited by 47 publications

(30 citation statements)

References 37 publications

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“…Previous studies have reported similar results (Jeannot et al, 2014;Santimaleeworagun et al, 2014;Khorsi et al, 2015;Mathlouthi et al, 2015;Memish et al, 2015;Wang et al, 2015). Khorsi et al (2015) analyzed the prevalence of A. baumannii multidrugresistance in hospitals in Algiers, and found that carbapenem resistance was mainly mediated by blaOXA-23 and blaOXA-24 genes.…”

Section: Discussionsupporting

confidence: 59%

Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China

Fang¹,

Wang²,

Dang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT.Acinetobacter baumannii is an aerobic non-motile Gramnegative coccobacillus, and it is one of the most important nosocomial pathogens worldwide. The aim of this study was to determine the molecular epidemiology of the outbreak strains. Between March 2011 and March 2014, a total of 205 strains of A. baumannii were isolated from patients at the Nanyang City Center Hospital. The blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 genes were amplified by multiplex polymerase chain reaction. We found that 68 (33.17%) strains were positive for the blaOXA-23 gene, and 88.24% of these 68 showed resistance to carbapenems, while 11.76% were sensitive to carbapenems. The blaOXA-51 gene was found in 132 (64.39%) strains, and 17.42% of these were resistant to carbapenems while 82.58% were sensitive to carbapenems. Moreover, 5 (2.44%) strains were positive for blaOXA-58, of which 80% were resistant to carbapenems and 20% were sensitive to carbapenems. We found that A. baumannii showed 100% drug resistance to ampicillin, cefotetan, cefazolin, and cefoperazone. Our findings suggest that the blaOXA-23 and blaOXA-51 genes are most frequently identified in A. baumannii, while blaOXA-23 is the most important gene for resistance to carbapenems.

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Section: Discussionsupporting

confidence: 59%

Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China

Fang¹,

Wang²,

Dang³

et al. 2016

Genet. Mol. Res.

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“…A total of 30 subtypes of given enzymes have been described, which are found in the following genera based on the BLDB database and literature search: Citrobacter, Escherichia, Enterobacter, Klebsiella, Serratia, Shewanella, and Acinetobacter. 49 Our primer was able to identify 27 subtypes, while not detecting the OXA-54 and OXA-535 enzymes described in Shewanella. New pairs of primers will need to be designed to detect them.…”

Section: Figmentioning

confidence: 84%

PCR Detection of Oxacillinases in Bacteria

Mlynárčik

Chalachanova

Vágnerová

et al. 2020

Microbial Drug Resistance

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Oxacillinases (OXA) have been mostly described in Enterobacteriaceae, Acinetobacter, and Pseudomonas species. Recent years have witnessed an increased prevalence of intrinsic and/or acquired b-lactamaseproducing Acinetobacter in food-producing animals. This study was conducted to assess the prevalence of OXA among selected bacterial species and to characterize these enzymes by in silico analysis. Screening of OXA was performed by PCR amplification using specific pairs of oligonucleotides. Overall, 40 pairs of primers were designed, of which 6 were experimentally tested in vitro. Among 49 bacterial isolates examined, the presence of bla OXA-1-like genes was confirmed in 20 cases (41%; 19 times in Klebsiella pneumoniae and once in Enterobacter cloacae). No OXA were found in animal isolates. The study results confirmed the specificity of the designed oligonucleotide pairs. Furthermore, the designed primers were found to possess the ability to specifically detect 90.2% of all OXA. These facts suggest that the in silico and in vitro tested primers could be used for single or multiplex PCR to screen for the presence of OXA in various bacteria, as well as to monitor their spread. At the same time, the presence of conserved characteristic amino acids and motifs was confirmed by in silico analysis of sequences of representative members of OXA.

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“…Other report have also demonstrated the presence of bla VIM-2 gene in P. aeruginosa and OXA-carbapenemase-encoding genes (i.e. bla OXA-23 , bla OXA-24 - and bla OXA-48 ) in A. baumannii isolates from hospitalized patients in a Libyan hospital [17]. …”

Section: Discussionmentioning

confidence: 99%

“…Recent and increasing reports have also documented the carbapenamase-producing bacteria, mainly involving Libyan traveller patients [6, 12-16]. These recent and limited studies have reported different carbapenemase-encoding genes in the Gram-negative bacteria, including the blaVIM-2, blaNDM and OXA-genes [17, 18]. Nevertheless, surveillance and epidemiological investigations on AMR bacterial pathogens in this particular region remain inadequate, limited and incomplete.…”

Section: Introductionmentioning

confidence: 99%

Current problematic and emergence of carbapenemase-producing bacteria: a brief report from a libyan hospital

Elramalli¹,

Almshawt²,

Ahmed³

2017

Pan Afr Med J

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A collection of 94 Gram-negative bacteria isolates, showing different antimicrobial resistance phenotypes including to the carbapenem classes was investigated. Strains were originated form clinical sources from a single hospital in Tripoli, Libya during 2015 and were identified based on cultural and phenotypic characteristics, and fully characterized by the VITEK automated system. Forty-eight percent (48%) of the collection was identified as Acinetobacter baumannii, 50% Klebsiella pneumoniae and 2% Escherichia coli. Resistance to the carbapenem classes was reported in 96% of the A. baumannii strains and 94% of the K. pneumonia strains. Seventy-eight percent (78%) of the isolates showed different multidrug-resistant (MDR) phenotypes, of which K. pneumoniae expressing the highest rates of MDRs(i.e. 91%). Emergence of resistance to carbapenems in the Gram-negative bacteria is a challenging global problem, particularly for Africa. Surveillance of these pathogens and appropriate actions are urgently required in Libyan healthcare settings.

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Emergence of Carbapenem-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Clinical Isolates Collected from Some Libyan Hospitals

Cited by 47 publications

References 37 publications

Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China

Molecular characterization of carbapenemase genes in Acinetobacter baumannii in China

PCR Detection of Oxacillinases in Bacteria

Current problematic and emergence of carbapenemase-producing bacteria: a brief report from a libyan hospital

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