Emergence of Bacterial Soft Rot in Cucumber Caused by Pectobacterium carotovorum subsp. brasiliense in China

Abstract

During 2014 to 2015, a devastating bacterial soft rot on cucumber stems and leaves occurred in Shandong, Shanxi, Hebei, Henan, and Liaoning provinces of China, resulting in serious economic losses for cucumber production. The gummosis emerged on the surface of leaves, stems, petioles, and fruit of cucumber. The basal stem color was dark brown and the stem base turned to wet rot. Yellow spots and wet rot emerged at the edge of the infected cucumber leaves and gradually infected the leaf centers. In total, 45 bacterial strains were isolated from the infected tissues. On the basis of phenotypic properties of morphology, physiology, biochemistry, and 16S ribosomal RNA gene sequence analysis, the pathogen was identified as Pectobacterium carotovorum. Multilocus sequence analysis confirmed that the isolates were P. carotovorum subsp. brasiliense, and the pathogens fell in clade II. The pathogenicity of isolated bacteria strains was confirmed. The strains reisolated were the same as the original. The host range test confirmed that strains had a wide range of hosts. As far as we know, this is the first report of cucumber stem soft rot caused by P. carotovorum subsp. brasiliense in China as well as in the world, which has a significant economic impact on cucumber production.

Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

“…P. brasiliense caused major economic losses to several crops (potato, cucumber, paprika, etc.) in many countries, including Canada, USA, South Africa, China, Korea and New Zealand [ 55 , 56 , 57 , 58 , 59 , 60 ]. The wide host range of this pathogen could facilitate its survival even in harsh environments, by parasitizing many alternative host plants.…”

Dynamic changes in bacterial communities in the recirculating nutrient solution of cucumber plug seedlings cultivated in an ebb-and-flow subirrigation system

“…lachrymans (causing bacterial angular leaf spot pathogen) [54], Erwinia tracheiphila (causing bacterial wilt) [55] and Pectobacterium carotovorum subsp. brasiliense (causing bacterial soft rot) [56], was detected in this ebb-and-flow system. Additionally, inoculation of the cucumber seedlings with microbes isolated from the effluent solution samples did not lead to any disease (data not shown).…”

Evaluation of a loop-mediated isothermal amplification assay based onhrpZgene for rapid detection and identification ofPseudomonas syringaepv.lachrymansin cucumber leaves

“…; Meng et al . ). Five strains belonging to Psl and 12 strains belonging to other species pathogens were used for the specificity test.…”

“…The specificity of the LAMP reaction was tested with DNA from different species of bacteria from the genera Pseudomonas, Xanthomonas, Acidovorax, Pectobacterium and Clavibacter (Table 1). Some strains within these genera can also infect cucumber (El-Hendawy 1999; Martin et al 1999;Meng et al 2016b). Five strains belonging to Psl and 12 strains belonging to other species pathogens were used for the specificity test.…”