Emergence of azole-resistant Candida albicans in small ruminants

Brilhante, Raimunda Sâmia Nogueira; Silva, S. T. C.; Castelo-Branco, Débora de Souza Collares Maia; Teixeira, Carlos Eduardo Cordeiro; Borges, L. C.; Bittencourt, Paula Vago; Oliveira, Jonathas Sales de; Monteiro, André Jalles; Bandeira, Tereza J. P. G.; Cordeiro, Rossana de Aguiar; Moreira, José Luciano Bezerra; Sidrim, José Júlio Costa; Rocha, Marcos F.G.

doi:10.1007/s11046-015-9888-z

Cited by 7 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, degradation of both resistant starch and fibres is expected to occur, as in monogastric vertebrates colon, as well as SCFA production and absorption by the colonic mucosa. A considerable amount of data have been collected on the composition and functions of sheep rumen microbiota (Shi et al ., ; Brilhante et al ., ; Morgavi et al ., ; Zeng et al ., ), but much less is currently known on the microbial populations that colonize the large intestine, despite their crucial role in the sheep intestinal metabolism.…”

Section: Introductionmentioning

confidence: 98%

Diversity and functions of the sheep faecal microbiota: a multi‐omic characterization

Tanca

Fraumene

Manghina

et al. 2017

Microbial Biotechnology

View full text Add to dashboard Cite

SummaryLittle is currently known on the microbial populations colonizing the sheep large intestine, despite their expected key role in host metabolism, physiology and immunity. This study reports the first characterization of the sheep faecal microbiota composition and functions, obtained through the application of a multi‐omic strategy. An optimized protocol was first devised for DNA extraction and amplification from sheep stool samples. Then, 16S rDNA sequencing, shotgun metagenomics and shotgun metaproteomics were applied to unravel taxonomy, genetic potential and actively expressed functions and pathways respectively. Under a taxonomic perspective, the sheep faecal microbiota appeared globally comparable to that of other ruminants, with Firmicutes being the main phylum. In functional terms, we detected 2097 gene and 441 protein families, finding that the sheep faecal microbiota was primarily involved in catabolism. We investigated carbohydrate transport and degradation activities and identified phylum‐specific pathways, such as methanogenesis for Euryarchaeota and acetogenesis for Firmicutes. Furthermore, our approach enabled the identification of proteins expressed by the eukaryotic component of the microbiota. Taken together, these findings unveil structure and role of the distal gut microbiota in sheep, and open the way to further studies aimed at elucidating its connections with management and dietary variables in sheep farming.

show abstract

Section: Introductionmentioning

confidence: 98%

Diversity and functions of the sheep faecal microbiota: a multi‐omic characterization

Tanca

Fraumene

Manghina

et al. 2017

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Studies with strains from animals, developed by our group, have detected the emergence of azole resistance in Candida spp. recovered from several animal species, especially C. albicans, demonstrating the importance of researches assessing this subject [12][13][14][15].…”

Section: Discussionmentioning

confidence: 99%

“…However, our group has recovered azole-resistant C. albicans from several animal species [12][13][14][15]. Therefore, this study aimed to reidentify these isolates, focusing on evaluating the accuracy of combined phenotypic tests for the differentiation of C. albicans and C. dubliniensis, using the polymerase chain reaction (PCR)-based identification as gold standard.…”

Section: Introductionmentioning

confidence: 99%

Combination of Phenotypic Tests as a Screening Approach for the Differentiation of Cryptic Species Candida albicans and Candida dubliniensis

Rocha¹,

Bandeira²,

Alencar³

et al. 2017

Med Mycol Open Access

View full text Add to dashboard Cite

show abstract

“…The emergence of resistance among C. albicans from animals is not well understood. Despite reports of resistance to azoles in these strains, the contribution of the efflux pump encoding genes CDR and MDR and the gene involved in ergosterol biosynthesis ERG11 had never been assessed in animal strains. It is hypothesised that the development of resistance in these fungi may be a way to adapt to stress in the environment and/or within animal hosts .…”

Section: Introductionmentioning

confidence: 99%

Azole resistance inCandida albicansfrom animals: Highlights on efflux pump activity and gene overexpression

Rocha

Bandeira

Alencar

et al. 2017

Mycoses

View full text Add to dashboard Cite

This study investigated potential mechanisms of azole resistance among Candida albicans from animals, including efflux pump activity, ergosterol content and gene expression. For this purpose, 30 azole-resistant C. albicans strains from animals were tested for their antifungal susceptibility, according to document M27-A3, efflux pump activity by rhodamine 6G test, ergosterol content and expression of the genes CDR1, CDR2, MDR1, ERG11 by RT-qPCR. These strains were resistant to at least one azole derivative. Resistance to fluconazole and itraconazole was detected in 23 and 26 strains respectively. Rhodamine 6G tests showed increased activity of efflux pumps in the resistant strains, showing a possible resistance mechanism. There was no difference in ergosterol content between resistant and susceptible strains, even after fluconazole exposure. From 30 strains, 22 (73.3%) resistant animal strains overexpressed one or more genes. From this group, 40.9% (9/22) overexpressed CDR1, 18.2% (4/22) overexpressed CDR2, 59.1% (13/22) overexpressed MDR1 and 54.5% (12/22) overexpressed ERG11. Concerning gene expression, a positive correlation was observed only between CDR1 and CDR2. Thus, azole resistance in C. albicans strains from animals is a multifactorial process that involves increased efflux pump activity and the overexpression of different genes.

show abstract

Emergence of azole-resistant Candida albicans in small ruminants

Cited by 7 publications

References 13 publications

Diversity and functions of the sheep faecal microbiota: a multi‐omic characterization

Diversity and functions of the sheep faecal microbiota: a multi‐omic characterization

Combination of Phenotypic Tests as a Screening Approach for the Differentiation of Cryptic Species Candida albicans and Candida dubliniensis

Azole resistance inCandida albicansfrom animals: Highlights on efflux pump activity and gene overexpression

Contact Info

Product

Resources

About