Emergence of a novel Enterobacter kobei clone carrying chromosomal-encoded CTX-M-12 with diversified pathogenicity in northeast China

Zhou, Kai; Yu, Wei; Bonnet, Richard; Cattoir, Vincent; Shen, Ping; Wang, Baohong; Rossen, John W. A.; Xiao, Yonghong

doi:10.1016/j.nmni.2017.01.006

Cited by 9 publications

(6 citation statements)

References 12 publications

(12 reference statements)

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“…According to the ANI results of 977 ECC genomes retrieved from the NCBI RefSeq database, the four species ( E. cloacae , E. hormaechei , E. roggenkampii , and E. kobei ) accounted for 82.7% of ECC population studied in this work, and most genomes were clinically associated, suggesting that the four species were predominant in the clinical setting. This is consistent with the previous studies ( Zhou et al., 2017 ; Davin-Regli et al., 2019 ; Wang et al., 2020 ). It is meaningful to develop a method which can identify ECC organisms efficiently and differentially, and has the potential to aid the control of four major ECC infections greatly.…”

Section: Discussionsupporting

confidence: 94%

“…Another important member of ECC is E. kobei . Clinical strains of E. kobei have been isolated from various clinical samples, including blood, sputum, urine, bronchoalveolar lavage, abscesses, and especially intra-abdominal samples ( Zhou et al., 2017 ; Davin-Regli et al., 2019 ). A recent study showed that E. hormaechei and E. kobei were the predominant ESBL-positive species accounting for more than 70% of community-acquired ECC strains collected across China ( Zhou et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (Enterobacter cloacae, Enterobacter hormaechei, Enterobacter roggenkampii, and Enterobacter kobei) Belonging to Enterobacter cloacae Complex With Clinical Significance

Wang

et al. 2021

Front. Cell. Infect. Microbiol.

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Enterobacter cloacae complex (ECC) is composed of multiple species and the taxonomic status is consecutively updated. In last decades ECC is frequently associated with multidrug resistance and become an important nosocomial pathogen. Currently, rapid and accurate identification of ECC to the species level remains a technical challenge, thus impedes our understanding of the population at the species level. Here, we aimed to develop a simple, reliable, and economical method to distinguish four epidemiologically prevalent species of ECC with clinical significance, i.e., E. cloacae, E. hormaechei, E. roggenkampii, and E. kobei. A total of 977 ECC genomes were retrieved from the GenBank, and unique gene for each species was obtained by core-genome comparisons. Four pairs of species-specific primers were designed based on the unique genes. A total of 231 ECC clinical strains were typed both by hsp60 typing and by species-specific PCRs. The specificity and sensitivity of the four species-specific PCRs ranged between 96.56% and 100% and between 76.47% and 100%, respectively. The PCR for E. cloacae showed the highest specificity and sensitivity. A one-step multiplex PCR was subsequently established by combining the species-specific primers. Additional 53 hsp60-typed ECC and 20 non-ECC isolates belonging to six species obtained from samples of patients, sewage water and feces of feeding animals were tested by the multiplex PCR. The identification results of both techniques were concordant. The multiplex PCR established in this study provides an accurate, expeditious, and cost-effective way for routine diagnosis and molecular surveillance of ECC strains at species level.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (Enterobacter cloacae, Enterobacter hormaechei, Enterobacter roggenkampii, and Enterobacter kobei) Belonging to Enterobacter cloacae Complex With Clinical Significance

Wang

et al. 2021

Front. Cell. Infect. Microbiol.

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“…The clinical significance of these strains is often uncertain. However, the species is now also of concern because of ESBL production (80).…”

Section: Enterobacter Cloacae Complexmentioning

confidence: 99%

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

2019

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SUMMARY The genus Enterobacter is a member of the ESKAPE group, which contains the major resistant bacterial pathogens. First described in 1960, this group member has proven to be more complex as a result of the exponential evolution of phenotypic and genotypic methods. Today, 22 species belong to the Enterobacter genus. These species are described in the environment and have been reported as opportunistic pathogens in plants, animals, and humans. The pathogenicity/virulence of this bacterium remains rather unclear due to the limited amount of work performed to date in this field. In contrast, its resistance against antibacterial agents has been extensively studied. In the face of antibiotic treatment, it is able to manage different mechanisms of resistance via various local and global regulator genes and the modulation of the expression of different proteins, including enzymes (β-lactamases, etc.) or membrane transporters, such as porins and efflux pumps. During various hospital outbreaks, the Enterobacter aerogenes and E. cloacae complex exhibited a multidrug-resistant phenotype, which has stimulated questions about the role of cascade regulation in the emergence of these well-adapted clones.

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“…These species are regularly isolated from clinical material and are involved in the development of opportunistic infections ( Raro et al., 2019 ; Arabaghian et al., 2019 ; Araujo et al., 2021 ). Likewise, Enterobacter species can cause opportunistic infections in human and animals ( Davin-Regli, Pagès, 2015 ; Zhou et al., 2017 ; Büyükcam et al., 2018 ; Annavajhala et al., 2019 ; Fuentes-Castillo et al., 2021 ). Citrobacter europaeus , isolated in this study from soil and dust samples of the intensive traffic area; was first isolated from the feces of a diarrheal patient and described in 2017 ( Ribeiro et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Enterobacteriaceae in soils and atmospheric dust aerosol accumulations of Moscow city

Глушакова

Качалкин

Прокофьева

et al. 2022

Current Research in Microbial Sciences

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Emergence of a novel Enterobacter kobei clone carrying chromosomal-encoded CTX-M-12 with diversified pathogenicity in northeast China

Abstract: International audienc

Cited by 9 publications

References 12 publications

Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (Enterobacter cloacae, Enterobacter hormaechei, Enterobacter roggenkampii, and Enterobacter kobei) Belonging to Enterobacter cloacae Complex With Clinical Significance

Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (Enterobacter cloacae, Enterobacter hormaechei, Enterobacter roggenkampii, and Enterobacter kobei) Belonging to Enterobacter cloacae Complex With Clinical Significance

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

Enterobacteriaceae in soils and atmospheric dust aerosol accumulations of Moscow city

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