Emergence and molecular characterization of multidrug-resistant &lt;em&gt;Klebsiella pneumoniae&lt;/em&gt; isolates harboring &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;CTX-M-15&lt;/sub&gt; extended-spectrum &amp;beta;-lactamases causing ventilator-associated pneumonia in China

Xu, Hui; Huo, Chunxiu; Sun, Yao; Zhou, Yiheng; Xiong, Yilin; Zhao, Zinan; Zhou, Qi; Li, Sha; Zhang, Beibei; Chen, Yang

doi:10.2147/idr.s189494

Cited by 15 publications

(13 citation statements)

References 41 publications

(42 reference statements)

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“…In addition, multiresistant strains increase the risk of infections caused by treatment failure in humans and animals. Multiresistant K. pneumonia isolates have emerged in many countries, including Northwest Iran, Turkey, Australia and China 25–28. Our study also showed a high occurrence of multiresistant K. pneumonia among humans and different animals, which suggest that these multiresistant strains can potentially be transmitted among humans and animals.…”

Section: Discussionsupporting

confidence: 57%

High rate of multiresistant Klebsiella pneumoniae from human and animal origin

Yang

Deng

Liao

et al. 2019

IDR

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Purpose The main objectives of the present study were to detect the antimicrobial susceptibility and molecular characteristics of Klebsiella pneumoniae isolated from different hosts and to investigate the possibility of K. pneumoniae transmission between animals and humans. Materials and methods A total of 189 nonduplicate K. pneumoniae isolates were collected from hospitals and four species of animals in Henan Province, China. The disk diffusion method was used for antimicrobial susceptibility testing, and resistance and virulence genes were screened by polymerase chain reaction (PCR). The molecular types were identified through multilocus sequence typing (MLST), and the hypermucoviscous (HMV) phenotype was identified using the “string-forming test”. Pearson’s parameters were used to determine the potential link among the molecular types and resistance and virulence genes of all K. pneumoniae strains. Results The resistance rates of the 189 K. pneumoniae isolates against 15 antibiotics ranged from 11.6% to 77.8%. The highest multidrug resistance rate was detected in the pig strains (93.6%), followed by the human strains (90.4%), chicken strains (88.9%), cow strains (52.0%) and sheep strains (50.0%). Forty-eight (25.4%) K. pneumoniae strains presented the HMV phenotype. entB, ﬁmH-1 and mrkD were the most prevalent of the detected virulence genes, and magA and rmpA were the least prevalent genes in all the isolates. The MLST analysis revealed 24 unique sequence types (STs) among from the 189 isolates. ST11, ST235 and ST258 were common STs among the five isolates of host origin. ST258 exhibited significantly positive correlations with blaNDM, magA and the HMV phenotype and a negative correlation with qnrB . Conclusion K. pneumoniae strains from different hosts, including humans and animals, have common molecular types and similar phenotypes, and these strains can potentially be transmitted between humans and animals.

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Section: Discussionsupporting

confidence: 57%

High rate of multiresistant Klebsiella pneumoniae from human and animal origin

Yang

Deng

Liao

et al. 2019

IDR

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“…In addition, isolate 1TR5R was susceptible to tobramycin, ceftazidime, and minocycline. Multidrug resistant K. pneumoniae has been reported by previous researchers [32,33], and is globally spreading unabated. This might lead to increased hospital stay and K. pneumoniae associated mortality.…”

Section: Antibiogram Of Colistin Resistant K Pneumoniae Isolatesmentioning

confidence: 91%

Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates

et al. 2021

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Multidrug resistant Enterobacterales have become a serious global health problem, with extended hospital stay and increased mortality. Antibiotic monotherapy has been reported ineffective against most drug resistant bacteria including Klebsiella pneumoniae, thus encouraging the use of multidrug therapies as an alternative antibacterial strategy. The present works assessed the antibacterial activity of colistin against K. pneumoniae isolates. Resistant isolates were tested against 16 conventional antibiotics alone and in combination with colistin. The results revealed that all colistin resistant isolates demonstrated multidrug resistance against the tested antibiotics except amikacin. At sub-inhibitory concentrations, combinations of colistin with amikacin, or fosfomycin showed synergism against 72.72% (8 of 11 isolates). Colistin with either of gentamicin, meropenem, cefoperazone, cefotaxime, ceftazidime, moxifloxacin, minocycline, or piperacillin exhibited synergism against 81.82% (9 of 11 isolates). Combinations of colistin with either of tobramycin or ciprofloxacin showed synergism against 45.45% (5 in 11 isolates), while combinations of colistin with imipenem or ceftolozane and tazobactam displayed 36.36% (4 of 11 isolates) and 63.64% (7 of 11 isolates) synergism. In addition, combinations of colistin with levofloxacin was synergistic against 90.91% (10 of 11 isolates). The results revealed that combinations of colistin with other antibiotics could effectively inhibit colistin resistant isolates of K. pneumoniae, and thus could be further explore for the treatment of multidrug resistant pathogens.

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“…Of note, we observed some known epidemic clones among the E. coli (i.e., ST10 and ST38) and K. pneumoniae (i.e., ST15 and ST307) isolates ( Woodford et al, 2011 ; Mathers et al, 2015 ; Navon-Venezia et al, 2017 ). K. pneumoniae ST15 isolates harboring bla CTX–M –15 , are emerging among patients with respiratory tract infections in China ( An et al, 2012 ; Xu et al, 2019 ) and have been previously isolated from companion animals in Paris ( Morcatti et al, 2018 ). As previously described, our data reveal clonal diversity among the recovered isolates and highlight Brazilian poultry meat as a reservoir of ExPEC lineages (i.e., ST10) ( Casella et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil

et al. 2021

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This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America.

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Emergence and molecular characterization of multidrug-resistant <em>Klebsiella pneumoniae</em> isolates harboring <em>bla</em><sub>CTX-M-15</sub> extended-spectrum β-lactamases causing ventilator-associated pneumonia in China

Cited by 15 publications

References 41 publications

<p>High rate of multiresistant <em>Klebsiella pneumoniae</em> from human and animal origin</p>

<p>High rate of multiresistant <em>Klebsiella pneumoniae</em> from human and animal origin</p>

Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates

Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil

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