<p>High rate of multiresistant <em>Klebsiella pneumoniae</em> from human and animal origin</p>

Abstract

Purpose The main objectives of the present study were to detect the antimicrobial susceptibility and molecular characteristics of Klebsiella pneumoniae isolated from different hosts and to investigate the possibility of K. pneumoniae transmission between animals and humans. Materials and methods A total of 189 nonduplicate K. pneumoniae isolates were collected from hospitals and four species of animals in Henan Province, China. The disk diffusion method was used for antimicrobial susceptibility testing, and resistance and virulence genes were screened by polymerase chain reaction (PCR). The molecular types were identified through multilocus sequence typing (MLST), and the hypermucoviscous (HMV) phenotype was identified using the “string-forming test”. Pearson’s parameters were used to determine the potential link among the molecular types and resistance and virulence genes of all K. pneumoniae strains. Results The resistance rates of the 189 K. pneumoniae isolates against 15 antibiotics ranged from 11.6% to 77.8%. The highest multidrug resistance rate was detected in the pig strains (93.6%), followed by the human strains (90.4%), chicken strains (88.9%), cow strains (52.0%) and sheep strains (50.0%). Forty-eight (25.4%) K. pneumoniae strains presented the HMV phenotype. entB, fimH-1 and mrkD were the most prevalent of the detected virulence genes, and magA and rmpA were the least prevalent genes in all the isolates. The MLST analysis revealed 24 unique sequence types (STs) among from the 189 isolates. ST11, ST235 and ST258 were common STs among the five isolates of host origin. ST258 exhibited significantly positive correlations with blaNDM, magA and the HMV phenotype and a negative correlation with qnrB . Conclusion K. pneumoniae strains from different hosts, including humans and animals, have common molecular types and similar phenotypes, and these strains can potentially be transmitted between humans and animals.

Emergence of Colistin and Carbapenem Resistance in Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae Isolated from Chickens and Humans in Egypt

“…In this study, K. pneumoniae was isolated from 9% of diseased chickens and 16.7% of environment samples, which is inconsistent with the findings reported in a previous study in Egypt, where K. pneumoniae was recovered from 35% of broiler chickens and 25% of water samples [ 42 ]. However, the prevalence of K. pneumoniae among contact poultry workers (22.7%) and hospitalized patients (14.4%) in the same region was lower than lately published reports in Egypt [ 7 , 42 , 43 ] or abroad, both in China [ 44 ] and Taiwan [ 45 ]. Hence, the direct transmission may be facilitated via close contact between chickens and humans as evidenced previously by Davis et al [ 46 ].…”

Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals

“…K. pneumoniae is a nosocomial pathogen with increasing MDR rates and global dissemination of high risk clones [ 70 ]. Although several studies have previously focused on K. pneumoniae in farm and companion animals [ 71 , 72 , 73 , 74 ], information about this pathogen in wildlife is still scarce [ 42 , 55 , 75 , 76 , 77 , 78 ]. Nevertheless, there are some reports about the isolation of K. pneumoniae from marine mammals along the U.S. Pacific Coast [ 19 , 79 , 80 , 81 ].…”

Antibiotics and Antibiotic Resistance Genes in Animal Manure – Consequences of Its Application in Agriculture

“…Although pathogens resistant to only one antibiotic may cause severe infections that are very hard to treat, MDR bacteria, such as those isolated from cattle feces, are particularly dangerous. Yang et al (2019) identified MDR in ESBL + K. pneumoniae isolated from cow feces. The strain showed resistance to 13 antibiotics from almost all commonly-used antimicrobial classes.…”