Emergence and characterisation of vanB vancomycin-resistant Enterococcus faecalis in Rio de Janeiro, Brazil

Merquior, Vânia Lúcia Carreira; Faria, Adriana Rocha; Rocha, Filomena Soares Pereira da; Morais, Jaqueline Martins; Neves, Felipe Piedade Gonçalves; Teixeira, Lúcia Martins

doi:10.1590/s0074-02762012000400020

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…In these experiments, we used V583, a vancomycin-resistant strain of E. faecalis (38). It should be noted that vancomycin-resistant E. faecalis strains represent only a minority of all vancomycin-resistant enterococci infections (39)(40)(41)(42)(43)(44)(45). However, risk factors for vancomycin-resistant E. faecalis acquisition include previous antibiotic exposure and invasive procedures (43,44), factors that are present in patients undergoing colorectal surgery.…”

Section: Discussionmentioning

confidence: 99%

Collagen degradation and MMP9 activation by Enterococcus faecalis contribute to intestinal anastomotic leak

et al. 2015

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Even under the most expert care, a properly constructed intestinal anastomosis can fail to heal resulting in leakage of its contents, peritonitis and sepsis. The cause of anastomotic leak remains unknown and its incidence has not changed in decades. Here, we demonstrate that the commensal bacterium Enterococcus faecalis contributes to the pathogenesis of anastomotic leak through its capacity to degrade collagen and to activate tissue matrix metalloprotease-9 (MMP9) in host intestinal tissues. We demonstrate in rats that leaking anastomotic tissues were colonized by E. faecalis strains that showed an increased collagen-degrading activity and also an increased ability to activate host MMP9, both of which contributed to anastomotic leakage. We demonstrate that the E. faecalis genes gelE and sprE were required for E. faecalis-mediated MMP9 activation. Either elimination of E. faecalis strains through direct topical antibiotics applied to rat intestinal tissues or pharmacological suppression of intestinal MMP9 activation prevented anastomotic leak in rats. In contrast, the standard recommended intravenous antibiotics used in patients undergoing colorectal surgery did not eliminate E. faecalis at anastomotic tissues nor did they prevent leak in our rat model. Finally, we show in humans undergoing colon surgery and treated with the standard recommended intravenous antibiotics, that their anastomotic tissues still contained E. faecalis and other bacterial strains with collagen-degrading/MMP9 activity. We suggest that intestinal microbes with the capacity to produce collagenases and to activate host metalloproteinase MMP9 may break down collagen in the gut tissue contributing to anastomotic leak.

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Section: Discussionmentioning

confidence: 99%

Collagen degradation and MMP9 activation by Enterococcus faecalis contribute to intestinal anastomotic leak

et al. 2015

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“…Gram-positive cocci infections such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus constitute a long-standing problem, but its real impact on hospital-acquired infections (HAI) has not been properly evaluated in our region. 12 …”

Section: Introductionmentioning

confidence: 99%

Online continuing interprofessional education on hospital-acquired infections for Latin America

Medina-Presentado

Margolis

Teixeira

et al. 2017

The Brazilian Journal of Infectious Diseases

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Implementation of this educational program showed the feasibility of a continent-wide interprofessional massive course on hospital acquired-infections in Latin America, in the two main languages spoken in the region. Next steps included a new edition of this course and a "New Challenges" course on hospital-acquired infections, which were successfully implemented in the second semester of 2015 by the same institutions.

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“…Os dados de estudos de vigilância global, mesmo diante das subnotificações indicam que a incidência da resistência microbiana aos antimicrobianos está aumentando exponencialmente PITTET, 2010;OHL;DODDS, 2011;ROSES PERIOGO, 2011;MERQUIOR et al, 2012;ANVARI, et al 2014).…”

Section: Introductionunclassified

Seguimento retrospectivo da sensibilidade de isolados clínicos aos antibióticos utilizados em um hospital terciário brasileiro de 2007 a 2012

Paula¹

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Emergence and characterisation of vanB vancomycin-resistant Enterococcus faecalis in Rio de Janeiro, Brazil

Cited by 6 publications

References 18 publications

Collagen degradation and MMP9 activation by Enterococcus faecalis contribute to intestinal anastomotic leak

Collagen degradation and MMP9 activation by Enterococcus faecalis contribute to intestinal anastomotic leak

Online continuing interprofessional education on hospital-acquired infections for Latin America

Seguimento retrospectivo da sensibilidade de isolados clínicos aos antibióticos utilizados em um hospital terciário brasileiro de 2007 a 2012

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