Embryotoxicity of polystyrene microplastics in zebrafish Danio rerio

Marco, Giuseppe De; Conti, Gea Oliveri; Giannetto, Alessia; Cappello, Tiziana; Galati, Mariachiara; Iaria, Carmelo; Pulvirenti, Eloise; Capparucci, Fabiano; Mauceri, Angela; Ferrante, Margherita; Maisano, Maria

doi:10.1016/j.envres.2021.112552

Cited by 83 publications

(25 citation statements)

References 55 publications

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“…While MPs are abundant in terrestrial environments, most studies to date have focused on MP effects on aquatic animals (e.g., Bringer et al, 2021 ; De Marco et al, 2022 ; Gaspar et al, 2018 ; Li et al, 2020 ; Lindeque et al, 2020 ). MPs are readily ingested by a variety of aquatic animals (e.g., invertebrates, sea birds, fish) and have a wide range of direct and indirect toxicities ( Anbumani and Kakkar, 2018 ; Cappello et al, 2021 ; Huang et al, 2021 ; Lusher et al, 2017 ; Weber et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Ingestion of Microplastic Fibres, But Not Microplastic Beads, Impacts Growth Rates in the Tropical House Cricket Gryllodes Sigillatus

et al. 2022

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Microplastic is a growing concern as an environmental contaminant as it is ubiquitous in our ecosystems. Microplastics are present in terrestrial environments, yet the majority of studies have focused on the adverse effects of microplastics on aquatic biota. We hypothesized that microplastic ingestion by a terrestrial insect would have localized effects on gut health and nutrient absorption, such that prolonged dietary microplastic exposure would impact growth rate and adult body size. We further hypothesized that plastic form (fibres vs. beads) would influence these effects because of the nature of gut-plastic interactions. Freshly hatched tropical house crickets (Gryllodes sigillatus) were fed a standard diet containing different concentrations of either fluorescent polyethylene microplastic beads (75–105 μm), or untreated polyethylene terephthalate microfibers (< 5 mm) until they died or reached adulthood (approximately 8 weeks). Weight and body length were measured weekly and microplastic ingestion was confirmed through fluorescence microscopy and visual inspection of the frass. While, to our surprise, we found no effect of polyethylene bead ingestion on growth rate or final body size of G. sigillatus, females experienced a reduction in size and weight when fed high concentrations of polyethylene terephthalate microfibers. These results suggest that high concentrations of polyethylene beads of the 100 μm size range can pass through the cricket gut without a substantial negative effect on their growth and development time, but high concentrations of polyethylene terephthalate microfibers cannot. Although we report the negative effects of microplastic ingestion on the growth of G. sigillatus, it remains uncertain what threats microplastics pose to terrestrial insects.

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Section: Introductionmentioning

confidence: 99%

Ingestion of Microplastic Fibres, But Not Microplastic Beads, Impacts Growth Rates in the Tropical House Cricket Gryllodes Sigillatus

et al. 2022

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“…[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45]). In particular, 11 studies (5 in vitro studies [29,33,37,38,42], 4 in vivo [27,30,32,36] and 2 both [34,39]) investigated exposure to PS , 3 studies investigated PP exposure (2 in vitro [37,41,42] and 1 in vivo [41]), 3 studies investigated PE exposure (1 in vitro [42] and 2 in vivo [28,31]), 2 studies investigated exposure to MPs (2 in vivo studies [44,45]) instead of PVC exposure (1 study in seen [3]), polymers (1 in vitro study [43]), LDPE (1 in vivo study [35]), HDPE (1 in vivo study [40]), SFb and LFb (1 in vivo study [41]), sphere (1 in vivo study [45]) and NPs (1 in vivo study [44]) was evaluated in a species-specific study.…”

Section: Resultsmentioning

confidence: 99%

“…Extreme solidity at room temperature, excellent electrical, thermal, and acoustic insulation, portability, ease of use thanks to the lightweight and cheapness are the main properties of plastic. These properties made it the most widespread and the most used synthetic material worldwide, causing a global pollution [1][2][3]. It was found in soil, sea, air, and also in the Arctic sea ice [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Nano and Microplastics on the Inflammatory Process: In Vitro and In Vivo Studies Systematic Review

Pulvirenti¹,

Ferrante²,

Barbera³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Microplastics (MPs) and Nanoplastics (NPs) are plastic fragments that spread in the environment and accumulate in the human body, so they have been becoming a worldwide environmental concern because of their potential human health effects. The aim of this systematic review was to investigate the prospective impact of MPs and NPs on the inflammatory process. Methods: Electronic article search was performed on PubMed, Scopus and Web of Science international databases from 1 Jan 2012 to 31 Dec 2021. Screenings of titles, abstracts and full texts were performed according to the Preferred Reporting Item for Systematic Review and Meta-analyses (PRISMA). The methodological quality of the studies was checked by the Toxicological data Reliability Assessment Tool. Results: Electronic article search identified 125 records, from which 6 in vitro, 11 in vivo and 2 both in vivo and in vitro studies were included. Both in vivo and in vitro studies have showed an increase ofdifferent inflammatory outcomes (Interleukines, Tumor necrosis factor, Chemokines, Interferones, Transcription factors, Growth factors, Oxydoreductase, Proteins and others), thus it seems to confirm the association withthe exposure to microplastics of different types, sizes, exposure times and exposed species. Conclusions: This systematic review seems to support the relationship between the exposure to MPs and the inflammatory processboth in vivo and in vitro. Greater caution is needed about the role of NPs because ofa very small number of studies. Additional high-quality studies are warranted to confirm these results, especially the research should be focused on NPs being lacking literature.

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“…After that, the samples were fixed in a paraffin wax and then slides with an approximately 5 µm thickness were cut using a microtome (Histo-line, MR2258, Thermo Fisher Scientific, Waltham, MA, USA). The tissue slides were deparaffinized and rehydrated using a descending grade of alcohol; i.e., from 100% to 30%, and then stained using hematoxylin and eosin (H&E staining) according to protocols described previously [ 53 , 54 , 55 ]. Histological observations were carried out on five fields of one section per sample at 400× magnification using a light microscope (MEIJI, Model: MT4300H, Saitama, Japan), equipped with a Canon digital camera (EOS 1300D) for acquisition of photographs.…”

Section: Methodsmentioning

confidence: 99%

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

Noureen

Rehman

Jabeen

et al. 2022

IJERPH

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Different types of metal oxide nanoparticles (NPs) are being used for wastewater treatment worldwide but concerns have been raised regarding their potential toxicities, especially toward non-targeted aquatic organisms including fishes. Therefore, the present study aimed to evaluate the toxicity of copper oxide (CuO) NPs (1.5 mg/L; positive control group) in a total of 130 common carp (Cyprinus carpio), as well as the potential ameliorative effects of fenugreek (Trigonella foenum-graecum) seed extracts (100 mg/L as G-1 group, 125 mg/L as G-2 group, and 150 mg/L as G-3 group) administered to fish for 28 days. Significant changes were observed in the morphometric parameters: the body weight and length of the CuO-NP-treated fish respectively decreased from 45.28 ± 0.34 g and 14.40 ± 0.56 cm at day one to 43.75 ± 0.41 g and 13.57 ± 0.67 cm at day 28. Conversely, fish treated with T. foenum-graecum seed extract showed significant improvements in body weight and length. After exposure to CuO NPs, a significant accumulation of Cu was recorded in the gills, livers, and kidneys (1.18 ± 0.006 µg/kg ww, 1.38 ± 0.006 µg/kg ww, and 0.05 ± 0.006 µg/kg ww, respectively) of the exposed common carp, and significant alterations in fish hematological parameters and oxidative stress biomarkers (lipid peroxidation (LPO), glutathione (GSH), and catalase (CAT)) were also observed. However, supplementing diets with fenugreek extracts modulated the blood parameters and the oxidative stress enzymes. Similarly, histological observations revealed that sub-lethal exposure to CuO NPs caused severe histomorphological changes in fish gills (i.e., degenerative epithelium, fused lamellae, necrotic lamellae, necrosis of primary lamellae, complete degeneration, and complete lamellar fusion), liver (i.e., degenerative hepatocytes, vacuolization, damaged central vein, dilated sinusoid, vacuolated degeneration, and complete degeneration), and kidney (i.e., necrosis and tubular degeneration, abnormal glomerulus, swollen tubules, and complete degeneration), while the treatment with the fenugreek extract significantly decreased tissue damage in a dose-dependent manner by lowering the accumulation of Cu in the selected fish tissues. Overall, this work demonstrated the ameliorative effects of dietary supplementation with T. foenum-graecum seed extract against the toxicity of NPs in aquatic organisms. The findings of this study therefore provided evidence of the promising nutraceutical value of fenugreek and enhanced its applicative potential in the sector of fish aquaculture, as it was shown to improve the growth performance and wellness of organisms.

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Embryotoxicity of polystyrene microplastics in zebrafish Danio rerio

Cited by 83 publications

References 55 publications

Ingestion of Microplastic Fibres, But Not Microplastic Beads, Impacts Growth Rates in the Tropical House Cricket Gryllodes Sigillatus

Ingestion of Microplastic Fibres, But Not Microplastic Beads, Impacts Growth Rates in the Tropical House Cricket Gryllodes Sigillatus

Effects of Nano and Microplastics on the Inflammatory Process: In Vitro and In Vivo Studies Systematic Review

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

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