Embryonic Stem Cells: New Possible Therapy for Degenerative Diseases That Affect Elderly People

He, Qing; Li, Jian; Bettiol, Esther; Jaconi, Marisa

doi:10.1093/gerona/58.3.m279

Cited by 18 publications

(11 citation statements)

References 94 publications

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“…For these reasons, ESCs hold enormous promise for cell replacement therapies or tissue repair in many degenerative disorders and injuries. However, amid controversy [4], ethical concerns [5], technical hurdles [6], and safety issues [7] over creating and testing hESC lines for transplantation therapies the FDA has been reluctant to approve any clinical trials in humans.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Canine Embryonic Stem Cell Lines Derived From Different Niche Microenvironments

Wilcox

Semple

Gartley

et al. 2009

Stem Cells and Development

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Embryo-derived stem cells hold enormous potential for producing cell-based transplantation therapies, allowing high-throughput drug screening and delineating early embryonic development. However, potential clinical applications must first be tested for safety and efficacy in preclinical animal models. Due to physiological and genetic parity to humans, the domestic dog is widely used as a clinically relevant animal model for cardiovascular, neurodegenerative, orthopedic, and oncologic diseases. Therefore, we established numerous putative canine embryonic stem cell (cESC) lines by immunodissection of the inner cell mass (ICM), which we termed OVC.ID.1-23, and by explant outgrowths from whole canine blastocysts, named OVC.EX.1-16. All characterized lines were immunopositive for OCT4, SOX2, NANOG, SSEA-3, and SSEA-4; displayed high telomerase and alkaline phosphatase (ALP) activities; and were maintained in this state up to 37 passages ( approximately 160 days). Colonies from OVC.EX lines showed classic domed hESC-like morphology surrounded by a ring of fibroblast-like cells, whereas all OVC.ID lines exhibited a mixed cell colony of tightly packed cESCs surrounded by a GATA6+/CDX2- hypoblast-derived support layer. Spontaneous serum-only differentiation without feeder layers demonstrated a strong lineage selection associated with the colony niche type, and not the isolation method. Upon differentiation, cESC lines formed embryoid bodies (EB) comprised of cells representative of all germinal layers, and differentiated into cell types of each layer. Canine ESC lines such as these have the potential to identify differences between embryonic stem cell line derivations, and to develop or to test cell-based transplantation therapies in the dog before attempting human clinical trials.

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Section: Introductionmentioning

confidence: 99%

Characterization of Canine Embryonic Stem Cell Lines Derived From Different Niche Microenvironments

Wilcox

Semple

Gartley

et al. 2009

Stem Cells and Development

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“…As human cells, murine embryonic stem cells can be cultured for an unlimited propagation (Hanna et al, 2010). To remain undifferentiated, murine cells needs some proteins, like leukemia inhibitory factor or murine embryonic fibroblasts (He et al, 2003). In the medical field, in order to detect and to confirm the source of a stem cell within different populations surface markers are used.…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

Strategies based on stem cells for tissue engineering

2018

Biomater Tissue Eng Bull

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Cell-therapy is a new approach in medical research field. The increased number of patients, which is reported all over the world, has led to the development of innovative solutions capable of improving wound healing. Stem cells, due to their self-renewal and differentiation capacities, gradually replace the traditional medicine used in the past. Even if the risks of using stem cells exists, the promising results they can offer have led to the design of intelligent materials that can make life easier for patients. Stem cells can be classified by many criteria, but the most important are embryonic and adult stem cells. The ability of embryonic stem cells of differentiate into any type of tissue by various strategies makes them an ideal candidate in tissue engineering applications. Ethical problems must be taken into consideration, so the most used in different therapies are adult stem cells. Nowadays, the applications of stem cells are various. They can be used in order to restore the function of soft and hard tissue and to improve the healing time of a wound. Skin problems, osteogenesis, angiogenesis or fracture healing are some examples of tissue disorders that can be restored by using stem cells therapy and nanotechnology.

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“…Earlier studies demonstrated potential regenerative therapies using ESC, which have the capability to differentiate into any cell type in our body [132]. This ESC concept was soon challenged by ethical concerns over destruction of life and the fact that the studies involve short-term culture of human embryos [128].…”

Section: Challenges and Future Directions Of Mesenchymal Stem Cell Thmentioning

confidence: 99%

Therapeutic Potential of Mesenchymal Stem Cells for Oral and Systemic Diseases

Kim

Mehrazarin

Kang

2012

Dental Clinics of North America

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Embryonic Stem Cells: New Possible Therapy for Degenerative Diseases That Affect Elderly People

Cited by 18 publications

References 94 publications

Characterization of Canine Embryonic Stem Cell Lines Derived From Different Niche Microenvironments

Characterization of Canine Embryonic Stem Cell Lines Derived From Different Niche Microenvironments

Strategies based on stem cells for tissue engineering

Therapeutic Potential of Mesenchymal Stem Cells for Oral and Systemic Diseases

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