Embryonic Stem Cell Marker Expression Pattern in Human Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, Heart and Dermis

Riekstiņa, Una; Čakstiņa, Inese; Parfejevs, Vadims; Hoogduijn, Martin J.; Jankovskis, G; Muižnieks, Indriķis; Muceniece, Ruta; Ancans, Janis

doi:10.1007/s12015-009-9094-9

Cited by 274 publications

(205 citation statements)

References 31 publications

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“…It is also difficult to obtain adult stem cells because of their limited quantities in the tissue. According to some previous literatures, they are considered to be composed of 0.01% to 0.001% of the total cell number (Hill et al, 2012;Rendl et al, 2005;Riekstina et al, 2009;Shim et al, 2013;Wegner and Stolt, 2005;Wu et al, 2010). We found that hDSPCs in small quantity gradually lose pluripotency and the ability to proliferate once they are grown over long-term culture.…”

Section: Introductionmentioning

confidence: 80%

“…SOX2, a SRY transcription factor, plays an essential role in maintaining stemness in embryonic stem cells and some adult stem cells such as the dermal papilla cells (Rendl et al, 2005;Wegner and Stolt, 2005). NANOG, another embryonic stem cell marker, is also expressed in adult mesenchymal stem cell populations derived from bone marrow, adipose tissue, and dermis (Hill et al, 2012;Riekstina et al, 2009;Szade et al, 2011). S100B are known to be specifically upregulated in dermal stem cells (Driskell et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O 2 concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.

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Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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“…VI (4) VII (19) VIII (20,21) IX (3,(22)(23)(24)(25) X (4) XI (3,(22)(23)(24)(25) XII (3,(22)(23)(24)(25) XIII ( (17) IV (4) V (4,18) VI (4) VII (19) VIII (20,21) IX (3,(22)(23)(24)(25) X (4) XI (3,(22)(23)(24)(25) XII (3,(22)(23)(24)(25) XIII (26) RHAMM/HMMR marker expression in the same stem cell lineage obtained from various tissues may indicate differences in their pluripotency. It is also possible that stem cell marker expression pattern and levels depend on respective cell cycle phases.…”

mentioning

confidence: 99%

Phenotypes of stem cells from diverse origin

2009

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“…Mitsui et al 46 found high concentrations of mRNA Nanog within the morula stage during embryonic development, and likewise, Tay et al 47 found that in the absence of OCT-3/4 and LIF, Nanog is capable of maintaining a pluripotent cell. Riekstina et al 48 al. 52 and Elloul et al 53 verified the presence of the expression of mesenchymal cell markers such as CD44, CD29, CD90 and coexpression with high participation for vimentin and Ck-Pan.…”

Section: Cell Culturementioning

confidence: 99%

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Filho¹,

Neto²,

Carvalho³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) -Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers -Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 10 6 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.Key words: Cell Culture Techniques. Cell Differentiation. Stem Cells. Flow Cytometry. Goats. Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

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Embryonic Stem Cell Marker Expression Pattern in Human Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, Heart and Dermis

Cited by 274 publications

References 31 publications

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Phenotypes of stem cells from diverse origin

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

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