Embryonic Expression Suggests an Important Role for CRP2/SmLIM in the Developing Cardiovascular System

Jain, Mukesh K.; Kashiki, Saori; Hsieh, Chung Ming; Layne, Matthew D.; Yet, Shaw Fang; Sibinga, Nicholas E.S.; Chin, Michael T.; Feinberg, Mark W.; Woo, Ian; Maas, Richard L.; Haber, Edgar; Lee, Mu En

doi:10.1161/01.res.83.10.980

Cited by 59 publications

(56 citation statements)

References 20 publications

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“…Instead, csrp2 expression was first observed in vascular SMCs at E11.5 ( Figure 3C). Continued csrp2 expression was detected in both vascular and venous SMCs through development ( Figures 3D, 3E, 3I, and 3L), as indicated in a previous report (Jain et al 1998). csrp2 gut and stomach epithelium expression was first noted at E13.5 ( Figure 3G) and continued at later stages (Figure 3I).…”

supporting

confidence: 84%

Expression of the Gene Encoding the LIM Protein CRP2: A Developmental Profile

Henderson

Brown

Richardson

et al. 2002

J Histochem Cytochem.

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(JAR) S U M M A R Y Members of the cysteine-rich protein (CRP) family are evolutionarily conserved L in-11, I sl-1, M ac-3 (LIM) domain proteins that have been implicated in cell differentiation. Here we describe the expression pattern of the CRP family member CRP2 in mouse. Unlike other CRP family members, which are expressed primarily in muscle, CRP2 is more broadly expressed. In addition to expression in vascular smooth muscle cells, we also detect CRP2-specific transcripts in mesenchymal derivatives and several epithelia.

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supporting

confidence: 84%

Expression of the Gene Encoding the LIM Protein CRP2: A Developmental Profile

Henderson

Brown

Richardson

et al. 2002

J Histochem Cytochem.

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“…6). So far, three distinct CRPs have been identified: CRP1 is expressed in visceral smooth-muscle cells [23], CRP2 in vascular smooth-muscle, whereas CRP3 is found in striated muscle only [24,25]. It is postulated that their main function is to contribute to cytoskeleton dynamics.…”

Section: Discussionmentioning

confidence: 99%

The human and mouse orthologous LIM-only proteins respectively encoded in chromosome 6 and 17 show a different expression pattern

Casrouge

Veitia

Kirchner

et al. 2004

Microbes and Infection

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Thymocytes interact with various subpopulations of thymic epithelial cells (TECs) at different stages of their development. To identify new molecules specifically expressed in TECs and/or thymic nurse cells (TNCs), we used representational difference analysis. We identified a LIM protein located on mouse chromosome 17 (m17TLP) and belonging to the family of the LIM-only proteins (LIMo). We found a new splice variant in addition to the two describedA and B isoforms. The three alternative species of m17TLP are found strictly in the thymic stroma. This protein is expressed on a subpopulation of TECs and TNCs. Strikingly, we found that the human ortholog of m17TLP, located on chromosome 6 (h6LIMo), is expressed in most tissues, but not in skeletal muscle. We have identified four human splice variants of h6LIMo which differ in their carboxy-terminal regions. The sequence comprising the genomic structure suggests that CRP2 is the closest known relative of m17TLP. Although the human and mouse nucleotide sequences are 88-97% homologous, this homology is reduced to 47% in the promoter regions, which strongly suggests that their differential expression is related to their promoter regulatory activity.

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“…All three CRP family members have been reported to localize to the nucleus (Chang et al, 2003;Kadrmas and Beckerle, 2004) and interact with ␣-actinin and zyxin (Schmeichel and Beckerle, 1994;Arber and Caroni, 1996;Louis et al, 1997;Pomies et al, 1997). Whereas CRP2 and CRP3 expression is limited to muscle cells (Jain et al, 1998), CRP1 is expressed in multiple adult organs and is the only member with detectable expression in the mammalian CNS (McLaughlin et al, 1994;Jain et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

CRP1, a Protein Localized in Filopodia of Growth Cones, Is Involved in Dendritic Growth

Ma¹,

Greenwood²,

Schachner³

2011

J. Neurosci.

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The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the only CRP family member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpin RNA interference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression of CRP1 with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca 2ϩ influx after depolarization. Ca 2ϩ /calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca 2ϩ -induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca 2ϩ influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

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Embryonic Expression Suggests an Important Role for CRP2/SmLIM in the Developing Cardiovascular System

Cited by 59 publications

References 20 publications

Expression of the Gene Encoding the LIM Protein CRP2: A Developmental Profile

Expression of the Gene Encoding the LIM Protein CRP2: A Developmental Profile

The human and mouse orthologous LIM-only proteins respectively encoded in chromosome 6 and 17 show a different expression pattern

CRP1, a Protein Localized in Filopodia of Growth Cones, Is Involved in Dendritic Growth

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