The human and mouse orthologous LIM-only proteins respectively encoded in chromosome 6 and 17 show a different expression pattern

Abstract

Thymocytes interact with various subpopulations of thymic epithelial cells (TECs) at different stages of their development. To identify new molecules specifically expressed in TECs and/or thymic nurse cells (TNCs), we used representational difference analysis. We identified a LIM protein located on mouse chromosome 17 (m17TLP) and belonging to the family of the LIM-only proteins (LIMo). We found a new splice variant in addition to the two describedA and B isoforms. The three alternative species of m17TLP are found strictly in the thymic stroma. This protein is expressed on a subpopulation of TECs and TNCs. Strikingly, we found that the human ortholog of m17TLP, located on chromosome 6 (h6LIMo), is expressed in most tissues, but not in skeletal muscle. We have identified four human splice variants of h6LIMo which differ in their carboxy-terminal regions. The sequence comprising the genomic structure suggests that CRP2 is the closest known relative of m17TLP. Although the human and mouse nucleotide sequences are 88-97% homologous, this homology is reduced to 47% in the promoter regions, which strongly suggests that their differential expression is related to their promoter regulatory activity.

Species-, organ- and cell-type-dependent expression of SPARCL1 in human and mouse tissues

“…Moreover, our findings suggest that it is highly recommended to perform systematic expression analyses in humans and mice before performing mechanistic mouse studies. Similar differences in the expression pattern of a protein in the human and murine organisms have been reported in a few previous studies, for example, for LIM-only proteins [31].…”

Comparative expression analysis of cysteine-rich intestinal protein family members crip1, 2 and 3 during Xenopus laevis embryogenesis

“…Human CRIP3 could be detected in the fetal thymus, spleen, brain, heart, kidney, liver and lung partially in line with our observations (Casrouge et al, 2004). These limited findings make a comparison of the expression across species difficult.…”

“…In adult mammals, all crip genes exhibit a wide tissue distribution, which might indicate essential roles in diverse cellular functions (Birkenmeier and Gordon, 1986, Casrouge et al, 2004, Chung et al, 2011, Karim et al, 1996, Kirchner et al, 2001, Levenson et al, 1993, Nalik et al, 1989, Okano et al, 1993, Tsui et al, 1994, van Ham et al, 2003, Wang et al, 1992, Yu et al, 2002. The different crip members have been linked to tissue differentiation and remodelling, immune response as well as suppression of angiogenesis and tumorigenesis (Chung et al, 2011, Davis et al, 1998, Lanningham-Foster et al, 2002, Wei et al, 2011.…”