Embryonal mitochondrial DNA: relationship to embryo quality and transfer outcomes

Klimczak, Amber M.; Pacheco, Lucia; Lewis, Kelsey; Massahi, N.; Richards, Jon P.; Kearns, W.G.; Saad, Antonio F.; Crochet, John R.

doi:10.1007/s10815-018-1147-z

Cited by 53 publications

(61 citation statements)

References 38 publications

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“…However, in this study we did not observe any correlation between maternal age (24-46 years) at the time of oocyte retrieval and mtDNA levels in trophectodermal cells of blastocyst. Similar to our findings, other studies also failed to report any correlation between maternal age with mtDNA levels in the blastocysts (de Los Santos et al, 2018; Diez-Juan et al, 2015; Klimczak et al, 2018; Victor et al, 2017). Together the results indicate, maternal age does not contribute to alterations in mtDNA levels in the blastocysts.…”

Section: Discussionsupporting

confidence: 92%

“…On the basis of this hypothesis, various studies have analyzed the levels of mtDNA and its association of the developmental competence. Studies have reported high levels of mtDNA in poor quality embryos (de Los Santos et al, 2018; Diez-Juan et al, 2015; Klimczak et al, 2018; Murakoshi et al, 2013). Interestingly, in our study, we did not find any correlation between morphological grading of blastocysts and mtDNA levels in the trophectodermal cells.…”

Section: Discussionmentioning

confidence: 99%

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No association of mitochondrial DNA levels in trophectodermal cells with the developmental competence of the blastocyst and pregnancy outcomes

Ritu

Veerasigamani

Ashraf

et al. 2019

Preprint

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17Study question 18 Can mitochondrial DNA (mtDNA) levels in trophectodermal cells of the blastocyst predict the 19 blastocyst quality, ploidy status, implantation rate and clinical outcomes? 20 Summary answer 21 mtDNA levels in trophectodermal cells of the blastocyst do not associate with the blastocyst 22 quality, ploidy status, implantation potential and clinical outcomes, but can differentiate between 23 aneuploid and euploid blastocysts.24 What we already know 25 mtDNA levels in the trophectodermal cells have been suggested to be associated with blastocyst 26 morphology, ploidy and implantation rates, and has been proposed as biomarker to access 27 blastocyst quality and predict clinical outcomes. However, discrepancies exist if mtDNA levels 28 could serve as a marker for the same.29 Study design and duration 30

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

No association of mitochondrial DNA levels in trophectodermal cells with the developmental competence of the blastocyst and pregnancy outcomes

Ritu

Veerasigamani

Ashraf

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Initial studies reported that an increased mtDNA copy number is related to aneuploidy and a decreased embryo implantation potential [23,26]. However, there have been other studies contradicting these findings [66,67]. All of these studies used different methodologies.…”

Section: Discussionmentioning

confidence: 99%

Parameters impacting the live birth rate per transfer after frozen single euploid blastocyst transfer

Boynukalın¹,

Gültomruk²,

Cavkaytar³

et al. 2020

PLoS ONE

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Background To assess the predictive value of patient characteristics, controlled ovarian stimulation and embryological parameters on the live birth outcome of single euploid frozen-warmed blastocyst transfer (FBT). Methods This was a retrospective cohort study including 707 single FBTs after preimplantation genetic testing for aneuploidy (PGT-A) that were performed from October 1, 2015, to January 1, 2018. The effects of patient-, cycle-and embryology-related parameters on the live birth outcome after FBT were assessed.

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“…This has led to speculation that IVF treatments could be optimized by screening for mtDNA content when embryos are being selected for implantation, rejecting all those with higher mitochondrial densities [135]. However, follow-up studies have failed to replicate these results, finding no association between the mtDNA content of early human blastocysts and either the age of the mother or the likelihood of the blastocyst becoming implanted [137,138]. At present, it seems likely that the discrepancies between studies are due to differences in the way in which samples are either collected, stored, or analysed [138,139], and the true relationship between maternal age and mitochondrial content of the early embryo remains to be resolved.…”

Section: (C) Mitochondrial Ageing In the Germlinementioning

confidence: 99%

The deteriorating soma and the indispensable germline: gamete senescence and offspring fitness

2019

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The idea that there is an impenetrable barrier that separates the germline and soma has shaped much thinking in evolutionary biology and in many other disciplines. However, recent research has revealed that the so-called ‘Weismann Barrier’ is leaky, and that information is transferred from soma to germline. Moreover, the germline itself is now known to age, and to be influenced by an age-related deterioration of the soma that houses and protects it. This could reduce the likelihood of successful reproduction by old individuals, but also lead to long-term deleterious consequences for any offspring that they do produce (including a shortened lifespan). Here, we review the evidence from a diverse and multidisciplinary literature for senescence in the germline and its consequences; we also examine the underlying mechanisms responsible, emphasizing changes in mutation rate, telomere loss, and impaired mitochondrial function in gametes. We consider the effect on life-history evolution, particularly reproductive scheduling and mate choice. Throughout, we draw attention to unresolved issues, new questions to consider, and areas where more research is needed. We also highlight the need for a more comparative approach that would reveal the diversity of processes that organisms have evolved to slow or halt age-related germline deterioration.

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Embryonal mitochondrial DNA: relationship to embryo quality and transfer outcomes

Cited by 53 publications

References 38 publications

No association of mitochondrial DNA levels in trophectodermal cells with the developmental competence of the blastocyst and pregnancy outcomes

No association of mitochondrial DNA levels in trophectodermal cells with the developmental competence of the blastocyst and pregnancy outcomes

Parameters impacting the live birth rate per transfer after frozen single euploid blastocyst transfer

The deteriorating soma and the indispensable germline: gamete senescence and offspring fitness

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