Embryogenic cells in plant tissue cultures: Occurrence and behavior

Carman, John G.

doi:10.1007/bf02623615

Cited by 120 publications

(62 citation statements)

References 103 publications

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“…Although various explants from many plants have been observed to produce somatic embryos the choice of explants is still a determinant factor (Williams and Maheswaran, 1986). Reports also abound that embryo development is dependent on reduction or absence of auxin in the induction medium (Carman, 1990). However, in the present study, production of FEC were significantly improved following subculture on GD medium supplemented with 500 µM tyrosine, suggesting that the OES from the two cassava cultivars responded optimally to tyrosine.…”

Section: Resultscontrasting

confidence: 51%

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Ubalua¹,

Mbanaso²

2014

J. Evol. Biol. Res.

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The embryogenic ability of two cassava cultivars (Sandpaper and TMS 60444) was investigated by culturing their young leaf lobes on an induction medium (DKW2 50P). The explants formed organized embryogenic structures regardless of the concentration of picloram in the medium. Organized embryogenic structures induction was enhanced by increasing the concentration of picloram in the induction medium for both cultivars. The optimum level of picloram at which maximum stable frequencies (53.1±17.6 and 51.5±14.6 for Sandpaper and TMS 60444 respectively) of organized embryogenic structures were obtained was 5 mg/l. Friable embryogenic callus were further produced in GD2 50P medium supplemented with 500 µM tyrosine. The produced friable embryogenic calluses are prime target tissues for genetic transformation and plantlet regeneration.

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Section: Resultscontrasting

confidence: 51%

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Ubalua¹,

Mbanaso²

2014

J. Evol. Biol. Res.

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“…Not all types of cells respond in the same way to the cultivate conditions and, for this reason, only some give the desired response in the culture. As pointed out by Carman (1990), embryogenic competence varies in different cells and may be related to gene activity that affects somatic embryogenesis, thus explaining why cells of the same explants possess different competence abilities.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Stein

Paiva

Vargas³

et al. 2010

Rev. Árvore

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-Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis. Keywords: Anthers, Ovaries e Nodal segments. ANÁLISE ULTRAESTRUTURAL DE CALOS DE INGAZEIRO (

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“…II existe done bien une relation entre Ia qualite des emhryons somati- Les conditions d'induction des cellules emhryogenes ne sont pas encore maitrisees. Bien qu'il soit possible chez Ia carotte (Smith et Krikorian, 1989) ou chez le cafeier d'obtenir des cellules embryogenes avec un milieu sans auxine, un stress auxinique est indispensable pour Ia plus grande majorite des especes (Carman, 1990). II peut etre tres bref comme chez la carotte ou au contraire s'etendre sur une longue duree : chez le palmier a huile, des repiquages tous les deux mois pendant une periode de 2 ans sur un milieu contenant du 2,4D ont parfois ete necessaires pour certains genotypes.…”

Section: Conclusion Et Discussionunclassified

Modalités d'initiation des cellules à l'origine des embryons somatiques

Michaux-Ferrière

Schwendiman

1993

Acta Botanica Gallica

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Embryogenic cells in plant tissue cultures: Occurrence and behavior

Cited by 120 publications

References 103 publications

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Modalités d'initiation des cellules à l'origine des embryons somatiques

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