Embryo Rescue of Solanum Lycopersicum × S. Peruvianum Tomato Cross for Tocv Resistance

Encina, Carlos López; Martín, E.; Fernández-Muñoz, Rafael

doi:10.17660/actahortic.2012.935.12

Cited by 6 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…() and Encina et al . () tried to characterize the inheritance of the ToCV resistance found in the line 802‐11‐1, originating from self‐pollinating cycles of the lineage IAC‐CN‐RT, by crossing with susceptible tomato cv. Moneymaker, but did not succeed due to crossing barriers.…”

Section: Discussionmentioning

confidence: 99%

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

et al. 2018

View full text Add to dashboard Cite

Tomato chlorosis virus (ToCV) is an emerging crinivirus in Brazil that causes an economically important disease in tomato (Solanum lycopersicum) and other solanaceous species. ToCV is transmitted predominantly by the whitefly Bemisia tabaci Middle East‐Asia Minor 1 (MEAM1, formerly biotype B), in a semipersistent manner. As all cultivated tomato varieties and hybrids are susceptible to this crinivirus, the main alternatives for the control of the disease are the use of healthy seedlings for transplanting and the chemical control of the insect vector. The objective of this work was to evaluate the responses of tomato genotypes to infection with this crinivirus and their tolerance to the disease in order to support the development of other alternatives for disease control. Resistance to infection was evaluated by ToCV inoculation with viruliferous B. tabaciMEAM1 followed by virus detection by RT‐PCR and RT‐qPCR. To measure tolerance to the disease, plant development and fruit yield of ToCV‐infected and healthy plants were compared. Among 56 genotypes, only the lineage IAC‐CN‐RT (S. lycopersicum ‘Angela Gigante’ × S. peruvianum ‘LA 444‐1’) was highly resistant to infection with ToCV. Tolerance to the disease over two trials with different genotypes showed variable results. The effect of ToCV on plant development varied from 2.9% to 71.9% reduction, while yield loss varied from 0.2% to 51.8%. The highly ToCV‐resistant lineage IAC‐CN‐RT, which is also resistant to a Spanish isolate of ToCV, might be useful for tomato breeding programmes.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

et al. 2018

View full text Add to dashboard Cite

show abstract

“…These barriers can be overcome by embryo rescue (Barbono and Topoleski, 1984, Pico et al, 2002). Several reports have been published about the successful development of interspecific hybrids by embryo rescue method and screening for Tospovirus resistance (Angora et al, 1981, Thomas and Pratt, 1981, Imanishi et al, 1985, Poysa, 1990, Chen and Adachi, 1992, Chen and Adachi, 1996, Segeren et al, 1993a, Segeren et al, 1993b, Bhattarai et al, 2009, Encina et al, 2012, Kharkongar et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

Sohrab

Bhattacharya

Rana

et al. 2015

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Tospovirus has emerged as a serious viral pathogen for several crops including tomato. The tomato production is being severely affected worldwide by Tospovirus. Some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. Resistance to this virus has been identified as good source in wild tomato species (Lycopersicum peruvianum). But the introgression of resistance genes into cultivated tomato lines and the development of interspecific hybrid are hampered due to incompatibility, fertilization barriers and embryo abortion. But this barrier has been broken by applying the embryo rescue methods. This study describes the development of interspecific hybrid tomato plants by highly efficient embryo rescue method and screening for Tospovirus resistance. The interspecific hybrid tomato plants were developed by making a cross between wild tomato species (L. peruvianum) and cultivated tomato (Solanum lycopersicum). The immature embryos were cultured in standardized medium and interspecific hybrids were developed from embryogenic callus. The immature embryos excised from 7 to 35 days old fruits were used for embryo rescue and 31 days old embryos showed very good germination capabilities and produced the highest number of plants. Developed plants were hardened enough and shifted to green house. The hybrid nature of interspecific plants was further confirmed by comparing the morphological characters from their parents. The F1, F2 and F3 plants were found to have varying characters especially for leaf type, color of stem, fruits, size, shapes and they were further screened for virus resistance both in lab and open field followed by Enzyme linked Immunosorbant Assay confirmation. Finally, a total of 11 resistant plants were selected bearing red color fruits with desired shape and size.

show abstract

“…Imanishi (1988) found that F1 and BC1 embryo culture had similar efficiencies and were unable to produce any BC1 plant from the hybrid plant they regenerated from callus culture. Also, Encina et al (2012) found extremely difficult to obtain the backcross to tomato. However, these results contrast with the ones obtained by Doganlar et al (1997).…”

Section: Discussionmentioning

confidence: 99%

“…En el transcurso de la obtención de estas generaciones se probaron los materiales por su resistencia a ambos virus, demostrado la introgresión con éxito de la misma (rendimiento = 0,6%), pseudo-FnBC1 (4%) y pseudo-FnBC2 (30%), demostrando la superación de las barreras de incompatibilidad a medida que se avanza en la obtención de retrocruces. El rendimiento del primer retrocruce (cruces realizados con parentales masculinos de la generación Fn) fue marcadamente bajo, coincidiendo con el obtenido por diversos autores (Imanishi, 1988;Encina et al, 2012). Inferior fue el resultado obtenido por , ya que no pudieron obtener ningún descendiente.…”

Section: Obtención De Generaciones Avanzadas a Partir De Solanum Peruunclassified

Aprovechamiento de las especies Solanum peruvianum en la mejora del tomate

Campos¹

View full text Add to dashboard Cite

Embryo Rescue of Solanum Lycopersicum × S. Peruvianum Tomato Cross for Tocv Resistance

Cited by 6 publications

References 0 publications

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

Screening tomato genotypes for resistance and tolerance to Tomato chlorosis virus

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

Aprovechamiento de las especies Solanum peruvianum en la mejora del tomate

Contact Info

Product

Resources

About