Embryo-culture in<i>Lathyrus</i>

Pecket, R. C.; Selim, Ashraf

doi:10.1093/jxb/16.2.325

Cited by 19 publications

(3 citation statements)

References 7 publications

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“…A high osmotic potential of the medium prevents precocious germination of young embryos and supports normal embryonic growth, and a higher concentration of sucrose is therefore often utilized for culturing immature plant embryos (Sharma et al 1996). A relatively high concentration of sucrose (60 g/L) is often used to culture the initial stage of immature grape embryos (Bharathy et al 2003(Bharathy et al , 2005Emershad and Ramming 1994;Guo et al 2004Guo et al , 2011bJi et al 2013b;Nookaraju et al 2007;Pommer et al 1995;Tang et al 2009;), but as the young embryos develop the osmotic potential of the medium is less important (Amemiya 1964;Niederwieser et al 1990;Pecket and Selim 1965;Sharma et al 1996) and the concentration of sucrose used for subsequent embryo germination and plantlet formation can be reduced (Bharathy et al 2003(Bharathy et al , 2005Emershad and Ramming 1994;Guo et al 2004Guo et al , 2011bNookaraju et al 2007;Pommer et al 1995;Tang et al 2009;Valdez 2005;Valdez and Ulanovsky, 1997;Wakana et al 2003;Xu et al 2005).…”

Section: Medium Compositionmentioning

confidence: 99%

Embryo rescue technique and its applications for seedless breeding in grape

Wang

et al. 2014

Plant Cell Tiss Organ Cult

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Grape (Vitis vinifera L.) is one of the most important fruit crops in the world and is subject to intense breeding efforts to develop new seedless cultivars. To overcome inherent obstacles associated with crossing seedless selections, in ovulo embryo rescue was developed and successfully utilized by plant breeders to rescue inherently weak, immature and/or abortive embryos, in order to obtain progeny from seedless 9 seedless crosses. To date, embryo rescue has been utilized in grape breeding for more than three decades. Genotype, sampling/inoculation time and medium are the most crucial and well-studied factors affecting the success of grape embryo rescue. Besides, other factors, such as the culture methods and utilization of plant growth regulators are also important for grape embryo rescue. Thus far, embryo rescue was extensively applied in rescuing inherently weak grape embryos, breeding seedless grapes and triploid grapes, and distant hybridization between different Vitis species. Although grape embryo rescue has been widely investigated, the development of improved cultivars is few. Breeding novel grape cultivars through embryo rescue is still a challenging and long-term task, which requires persistent effort of grape breeders. This review provides updated and comprehensive information concerning factors and applications of embryo rescue in grape.

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Section: Medium Compositionmentioning

confidence: 99%

Embryo rescue technique and its applications for seedless breeding in grape

Wang

et al. 2014

Plant Cell Tiss Organ Cult

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“…Young embryos of Lathyrus (Pecket & Selim, 1965) had a clear requirement for high concentrations of sucrose which was osmotic in nature. Upon transfer of the embryos from the high sucrose medium to a lower concentration of sucrose, embryos initially 2 mm.…”

Section: In Vitromentioning

confidence: 99%

Addendum

1966

Biological Reviews

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“…Interspecific hybrid embryo culture has been successful in other Leguminosae species such as Arachis [1], Glycine [14], Lathyrus [16], Lotus [9], Melilotus [26], Ornithropus [27], Trifolium [18] and Vigna [8].…”

Section: Introductionmentioning

confidence: 99%

Embryo culture of Medicago scutellata and M. sativa

Bauchan

1987

Plant Cell Tiss Organ Cult

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Resistance to the alfalfa weevil (Hypera postica (Gyllenhal)) and the potato leafhopper (Empoasco fabae (Harris)) is lacking in cultivated alfalfa. However, a closely related annual Medicago, Medicago scutellata¢ possesses dense glandular stem and leaf hairswhich provides a mechanism for resistance. Several attempts have been made at transfering the glandular haired trait from M. seutellata to perennial alfalfa with limited success. Earlier studies have shown that one reason for the lack of success is embryo abortion. Therefore, this study was initiated to observe zygotic embryo-genesis and to develop an embryo rescue technique for M. scutellata and M. sativa. Observations of zygotic embryogenesis showed that the two species are similar in morphology and can be described from youngest to oldest as globular, heart, torpedo, and hook shaped embryos. M. sativa embryos are smaller than M. scutellata embryos and develop three to four days later. Self pollinated M. scutellata (PI 307446) and sib mated M. sativa (Saranac AR) embryos were cultivated on Murashige and Skoog (MS) basal medium with various combination of 2,4-dichlorophenoxyacetic acid (2,4-D), indolacetic acid (IAA), 6-benzylaminopurine (BAP), and kinetic (KIN). Embryos from both species were also cultured on Schenk and Hildebrandt's (SH) basal medium with the addition of L-glutamine and L-proline. The experimental design was a completely randomized factorial for each experiment. Heart and torpedo shaped embryos from M. scutellata grew best (27.5% plantlet recovery) when cultured on MS medium with 0.05 mgl -t of both IAA and BAP. After 15 to 30 days on this medium, the embryos had only developed shoots. Therefore, it was necessary to transfer the shoots to MS basal medium without phytohormones for rooting. Rooting occurred in 15 to 30 days and the plantlets could be acclimatized to soil within 2 to 4 weeks. M. sativa embryos grew best (31% plantlet recovery~ on SH medium with 50 mM L-glutamine. M. sativa embryos developed both shoots and roots on this medium. This information may now be applied to the development of an embryo culture method for recovering insect resistant hybrids between M. scutellata and M. sativa.

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Embryo-culture inLathyrus

Cited by 19 publications

References 7 publications

Embryo rescue technique and its applications for seedless breeding in grape

Embryo rescue technique and its applications for seedless breeding in grape

Addendum

Embryo culture of Medicago scutellata and M. sativa

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