Embolization of a common carotid aneurysm with rhVEGF coupled to a pH-responsive chitosan in a rat model

Pan, Hui; Zimmerman, Thomas; Zakhaleva, Julia; Abrahams, John M.; Jiang, Hongliang; Chen, Weiliam

doi:10.3171/2009.1.jns08411

Cited by 4 publications

(2 citation statements)

References 16 publications

(22 reference statements)

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“…However, improvements are required before clinical application. The short half-life and instability being interfered by endogenous substances remain problematic, preventing VEGF-coated coils from exerting biological effects [20].…”

Section: Discussionmentioning

confidence: 99%

“…Fifty-four healthy adult female Sprague-Dawley rats (200-250 g) were used in this study and randomly divided into three groups: i) P(DLLA-co-TMC)/VEGF-coated coil segment group; ii) P(DLLA-co-TMC)/heparin-coated coil segment group; iii) uncoated coil segment group. The details of aneurysm model construction have been previously described [9,16,20]. Briefly, after anesthesia by intraperitoneal administration of 60 mg/kg sodium pentobarbital, the rats were placed in the supine position, and a midline skin incision was made in the anterior neck.…”

Section: Aneurysm Creation and Coil Placementmentioning

confidence: 99%

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Acceleration of aneurysm healing by P(DLLA-co-TMC)-coated coils enabling the controlled release of vascular endothelial growth factor

et al. 2014

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Since the introduction of the detachable coil in endovascular treatment of intracranial aneurysms, the in-hospital mortality rate has been significantly decreased. Recurrence of the aneurysm remains the major drawback of using detachable coils. We prepared a bioactive coil coated with poly(d,l-lactide)-7co-(1,3-trimethylene carbonate) (P(DLLA-co-TMC)), a novel copolymer for controlling the release of vascular endothelial growth factor (VEGF). Platinum coils were prepared by successive coating with cationic P(DLLA-co-TMC) and anionic heparin. Then, recombinant human VEGF-165 (rhVEGF) was immobilized by affinity binding to heparin. The morphological characteristics and sustained in vitro release of rhVEGF were examined using scanning electron microscopy and enzyme-linked immunosorbent assay, respectively. The efficacy of these novel coils modified by P(DLLA-co-TMC)/rhVEGF was tested using a common carotid artery aneurysm model in rats. Experimental aneurysms were embolized with unmodified, P(DLLA-co-TMC)/heparin-coated or P(DLLA-co-TMC)/rhVEGF-coated platinum coils (n = 18). The coils were removed on days 15, 30 and 90 after insertion, and the histological and immunohistochemical analysis of factor VIII was performed to confirm the presence of endothelial cells in the organized area. In addition, the controlled in vivo release of VEGF was confirmed by Western blotting analysis. The release of VEGF tended to increase during the whole period and no burst release was observed. In the group treated with P(DLLA-co-TMC)/rhVEGF-coated platinum coils, clot organization and endothelial cell proliferation were accelerated. The immunohistochemistry study showed that the expression of factor VIII was found in the P(DLLA-co-TMC)/rhVEGF-coated coil group but not in the other two groups. Furthermore, Western blotting analysis confirmed that the major released VEGF in the aneurysm sac was from the P(DLLA-co-TMC)/VEGF-coated coil. P(DLLA-co-TMC)/rhVEGF-coated platinum coils can promote clot organization and endothelial cell proliferation in a rat aneurysm model.

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Section: Discussionmentioning

confidence: 99%

Section: Aneurysm Creation and Coil Placementmentioning

confidence: 99%

Acceleration of aneurysm healing by P(DLLA-co-TMC)-coated coils enabling the controlled release of vascular endothelial growth factor

et al. 2014

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Injectable hydrogels for vascular embolization and cell delivery: The potential for advances in cerebral aneurysm treatment

Kim¹,

Nowicki²,

Gross³

et al. 2021

Biomaterials

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Aspirin and Clopidogrel Inhibit Aneurysm Healing after HydroCoil Implantation in External Carotid Artery Aneurysm Model

Zhang

et al. 2016

Journal of Stroke and Cerebrovascular Diseases

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Embolization of a common carotid aneurysm with rhVEGF coupled to a pH-responsive chitosan in a rat model

Abstract: Chitosan with a bioactive agent such as rhVEGF showed excellent results in occluding aneurysms in a rat model.

Cited by 4 publications

References 16 publications

Acceleration of aneurysm healing by P(DLLA-co-TMC)-coated coils enabling the controlled release of vascular endothelial growth factor

Acceleration of aneurysm healing by P(DLLA-co-TMC)-coated coils enabling the controlled release of vascular endothelial growth factor

Injectable hydrogels for vascular embolization and cell delivery: The potential for advances in cerebral aneurysm treatment

Aspirin and Clopidogrel Inhibit Aneurysm Healing after HydroCoil Implantation in External Carotid Artery Aneurysm Model

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