Embedding of Precision-Cut Lung Slices in Engineered Hydrogel Biomaterials Supports Extended <i>Ex Vivo</i> Culture

Bailey, Kolene E.; Pino, Christopher J.; Lennon, Mallory L; Lyons, Anne C.; Jacot, Jeffrey G.; Lammers, Steven R.; Königshoff, Mélanie; Magin, Chelsea M.

doi:10.1165/rcmb.2019-0232ma

Cited by 46 publications

(48 citation statements)

References 43 publications

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“…Indeed, recent studies could show that human PCLS are viable, preserve structural integrity, contain all lung-relevant cell types and are metabolically active for up to 15 days of cultivation 12,13,36 . Recently, it could also been shown, that the embedding of PCLS into specifically engineered hydrogels is able to significantly increase viability and pneumocyte type 2 functionality over a course of 21 days of cultivation when compared with non-encapsulated controls 37 . Furthermore, human PCLS showed cytotoxic, inflammatory and immune responses to selected stimuli (e.g.…”

Section: Introductionmentioning

confidence: 99%

The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices

Preuß

Schubert

Werlein

et al. 2022

The American Journal of Pathology

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Section: Introductionmentioning

confidence: 99%

The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices

Preuß

Schubert

Werlein

et al. 2022

The American Journal of Pathology

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“…Bailey et al systematically evaluated PEG-based hydrogels for supporting extended ex vivo culture of precision-cut lung slices, specifically the maintenance of alveolar epithelial type II (ATII) cells, a progenitor cell capable of differentiation into alveolar epithelial type I (ATI) cells, the cells lining the gas exchange surfaces of the lung. This research demonstrated that incorporation of two short peptide sequences that bind β 1 -class integrins (0.2 mM YIGSR and 0.1 mM RGDS) supported production of surfactant protein c, that is, maintained the functionality of ATII cells within PLCS for up to 21 days, in contrast to unembedded controls that only survive ∼7 days in culture (53). In a recent example of pulmonary disease modeling, a dynamically responsive PEG-α-methacrylate (PEGαMA) hybrid-hydrogel containing proteins from decellularized lung extracellular matrix was stiffened in situ using light to increase the elastic modulus of the material from healthy (E = 3.6 ± 0.24 kPa) to fibrotic ranges (E = 13.4 ± 0.82 kPa).…”

Section: Opportunities For Tissue-informed Biomaterials To Advance Pulmonary Regenerative Medicinementioning

confidence: 92%

Engineering Tissue-Informed Biomaterials to Advance Pulmonary Regenerative Medicine

et al. 2021

Self Cite

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Biomaterials intentionally designed to support the expansion, differentiation, and three-dimensional (3D) culture of induced-pluripotent stem cells (iPSCs) may pave the way to cell-based therapies for chronic respiratory diseases. These conditions are endured by millions of people worldwide and represent a significant cause of morbidity and mortality. Currently, there are no effective treatments for the majority of advanced lung diseases and lung transplantation remains the only hope for many chronically ill patients. Key opinion leaders speculate that the novel coronavirus, COVID-19, may lead to long-term lung damage, further exacerbating the need for regenerative therapies. New strategies for regenerative cell-based therapies harness the differentiation capability of human iPSCs for studying pulmonary disease pathogenesis and treatment. Excitingly, biomaterials are a cell culture platform that can be precisely designed to direct stem cell differentiation. Here, we present a closer look at the state-of-the-art of iPSC differentiation for pulmonary engineering, offer evidence supporting the power of biomaterials to improve stem cell differentiation, and discuss our perspective on the potential for tissue-informed biomaterials to transform pulmonary regenerative medicine.

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“…Generally, PCLS can survive for a period of up to 7 days [ 179 ]. However, the cultivation period can be prolonged for up to ~14 days [ 180 , 181 , 182 ] or even 21 days [ 183 ] when cultured in appropriate media systems. Longer cultivation of PCLS is essential for chronic exposure experiments.…”

Section: Cellular Evaluation Of Drug Delivery System For Lung Cancermentioning

confidence: 99%

Advanced Nanoparticle-Based Drug Delivery Systems and Their Cellular Evaluation for Non-Small Cell Lung Cancer Treatment

Mohtar

Parumasivam

Gazzali

et al. 2021

Cancers

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Lung cancers, the number one cancer killer, can be broadly divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), with NSCLC being the most commonly diagnosed type. Anticancer agents for NSCLC suffer from various limitations that can be partly overcome by the application of nanomedicines. Nanoparticles is a branch within nanomedicine that can improve the delivery of anticancer drugs, whilst ensuring the stability and sufficient bioavailability following administration. There are many publications available in the literature exploring different types of nanoparticles from different materials. The effectiveness of a treatment option needs to be validated in suitable in vitro and/or in vivo models. This includes the developed nanoparticles, to prove their safety and efficacy. Many researchers have turned towards in vitro models that use normal cells or specific cells from diseased tissues. However, in cellular works, the physiological dynamics that is available in the body could not be mimicked entirely, and hence, there is still possible development of false positive or false negative results from the in vitro models. This article provides an overview of NSCLC, the different nanoparticles available to date, and in vitro evaluation of the nanoparticles. Different types of cells suitable for in vitro study and the important precautions to limit the development of false results are also extensively discussed.

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Embedding of Precision-Cut Lung Slices in Engineered Hydrogel Biomaterials Supports Extended Ex Vivo Culture

Cited by 46 publications

References 43 publications

The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices

The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices

Engineering Tissue-Informed Biomaterials to Advance Pulmonary Regenerative Medicine

Advanced Nanoparticle-Based Drug Delivery Systems and Their Cellular Evaluation for Non-Small Cell Lung Cancer Treatment

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