Elutive displacement of precipitate formed during electromigration of ions

Deman, Joris

doi:10.1021/ac50160a034

Cited by 14 publications

(13 citation statements)

References 3 publications

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“…In 1970, Deman et al [19,20] advanced the important concepts of ''precipitate reaction front'' and ''moving reaction front.'' At the same time, Deman [21,22] observed the elution displacement of metal ions and the separation of six rare earth ions in his experiments. In 1990s, the idea of equivalent electromigration reaction between H 1 and OH À was developed for protein concentration [23].…”

Section: Introductionmentioning

confidence: 93%

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Yang¹,

Fan²,

Shan-sheng³

et al. 2011

Electrophoresis

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In this paper, we developed a novel method of acid-base titration, viz. the electromigration acid-base titration (EABT), via a moving neutralization boundary (MNR). With HCl and NaOH as the model strong acid and base, respectively, we conducted the experiments on the EABT via the method of moving neutralization boundary for the first time. The experiments revealed that (i) the concentration of agarose gel, the voltage used and the content of background electrolyte (KCl) had evident influence on the boundary movement; (ii) the movement length was a function of the running time under the constant acid and base concentrations; and (iii) there was a good linearity between the length and natural logarithmic concentration of HCl under the optimized conditions, and the linearity could be used to detect the concentration of acid. The experiments further manifested that (i) the RSD values of intra-day and inter-day runs were less than 1.59 and 3.76%, respectively, indicating similar precision and stability in capillary electrophoresis or HPLC; (ii) the indicators with different pK(a) values had no obvious effect on EABT, distinguishing strong influence on the judgment of equivalence-point titration in the classic one; and (iii) the constant equivalence-point titration always existed in the EABT, rather than the classic volumetric analysis. Additionally, the EABT could be put to good use for the determination of actual acid concentrations. The experimental results achieved herein showed a new general guidance for the development of classic volumetric analysis and element (e.g. nitrogen) content analysis in protein chemistry.

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Section: Introductionmentioning

confidence: 93%

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Yang¹,

Fan²,

Shan-sheng³

et al. 2011

Electrophoresis

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“…In addition, moving reaction boundary equations could be deduced to describe the quantitative relations (mass balance) for the substances present on both sides of electrophoretic boundary. Since the concept of MRB introduced in 1970, 13,14 moving reaction boundaries based on different chemical reactions have been investigated. However, moving redox reaction boundary is seldom discussed, which is probably due to the more complicated reaction mechanism, relatively slow reaction rate and rigorous reaction medium.…”

Section: Introductionmentioning

confidence: 99%

Model creation of moving redox reaction boundary in agarose gel electrophoresis by traditional potassium permanganate method

Xie

Liu

et al. 2013

Analyst

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A novel moving redox reaction boundary (MRRB) model was developed for studying electrophoretic behaviors of analytes involving redox reaction on the principle of moving reaction boundary (MRB). Traditional potassium permanganate method was used to create the boundary model in agarose gel electrophoresis because of the rapid reaction rate associated with MnO(4)(-) ions and Fe(2+) ions. MRB velocity equation was proposed to describe the general functional relationship between velocity of moving redox reaction boundary (V(MRRB)) and concentration of reactant, and can be extrapolated to similar MRB techniques. Parameters affecting the redox reaction boundary were investigated in detail. Under the selected conditions, good linear relationship between boundary movement distance and time were obtained. The potential application of MRRB in electromigration redox reaction titration was performed in two different concentration levels. The precision of the V(MRRB) was studied and the relative standard deviations were below 8.1%, illustrating the good repeatability achieved in this experiment. The proposed MRRB model enriches the MRB theory and also provides a feasible realization of manual control of redox reaction process in electrophoretic analysis.

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“…The main characteristic and trait of MRB is the presence of the reaction such as the neutralization reaction between hydrogen ion and hydroxyl ion, the chelation reaction between the chelator and metal ions, the precipitate reaction between metal ions and hydroxyl ion, the affinity reaction between Ni(II) and histidine, and so on. In 1970, Deman and Rigole [34][35][36][37] advanced the important ideas of the 'precipitate reaction front' and 'moving reaction front' (MRF), developed the concept of elution boundary (EB) and performed the EB-induced separation of metal ions in a MRF. In 1993, Pospichal et al 38 advanced the concept of stationary neutralization boundary in electrofocusing apparatus of CE.…”

Section: Introductionmentioning

confidence: 99%

“…In 1997-2008, Cao et al [39][40][41] developed the concept of MRB for isoelectric focusing (IEF) and sample stacking in CE. Up to now, six kinds of MRB systems have been developed by the authors, including the moving precipitate boundary, [34][35][36][37]39 moving neutralization boundary, [38][39][40][41] moving chelation boundary (MCB), 40,42 moving oxidation-reduction boundary 40,43 and moving affinity boundary 44 as well as moving supramolecular or interaction boundary. 40,45 A novel MRB-based ITP mode has been observed in the experiments on MCB.…”

Section: Introductionmentioning

confidence: 99%

Theoretical and experimental studies on isotachophoresis in multi-moving chelation boundary system formed with metal ions and EDTA

Zhang

Guo

Fan

et al. 2013

Analyst

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In this paper, a general mode and theory of moving chelation boundary based isotachophoresis (MCB-based ITP), together with the concept of decisive metal ion (DMI) having the maximum complexation constant (lg Kmax) with the chelator, were developed from a multi-MCB (mMCB) system. The theoretical deductions were: (i) the reaction boundary velocities in the mMCB system at steady state were equal to each other, resulting in a novel MCB-based ITP separation of metal ions; (ii) the boundary directions and velocities in the system were controlled by the fluxes of chelator and DMI, rather than other metal ions; and (iii) a controllable stacking of metal ions could be simultaneously achieved in the developed system. To demonstrate the deductions, a series of experiments were conducted by using model chelator of EDTA and metal ions of Cu(II) and Co(II) due to characteristic colors of blue [Cu-EDTA](2-) and pink [Co-EDTA](2-) complexes. The experiments demonstrated the correctness of theoretical deductions, indicating the validity of the developed model and theory of ITP. These findings provide guidance for the development of MRB-based ITP separation and stacking of metal ions in biological sample matrix and heavy metal ions in environmental samples.

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Elutive displacement of precipitate formed during electromigration of ions

Cited by 14 publications

References 3 publications

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Equivalence‐point electromigration acid–base titration via moving neutralization boundary electrophoresis

Model creation of moving redox reaction boundary in agarose gel electrophoresis by traditional potassium permanganate method

Theoretical and experimental studies on isotachophoresis in multi-moving chelation boundary system formed with metal ions and EDTA

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