Elution of viruses by ionic and nonionic surfactants

Fujito, B T; Lytle, C. David

doi:10.1128/aem.62.9.3470-3473.1996

Cited by 22 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The influence of surfactant type (ionic and nonionic) and membrane binding type on the elution of two viruses (phi Xl74 410 and PR01) was investigated by Fujito and Lytle (1996). Complete elution of the virus only was achieved using matched combinations of surfactants and binding membranes.…”

Section: Virusesmentioning

confidence: 99%

An Analytical Method for Trifluoroacetic Acid in Water and Air Samples Using Headspace Gas Chromatographic Determination of the Methyl Ester

Zehavi

Seiber

1996

Anal. Chem.

View full text Add to dashboard Cite

An analytical method has been developed for the determination of trace levels of trifluoroacetic acid (TFA), an atmospheric breakdown product of several of the hydrofluorocarbon (HFC) and hydrochlorofluorocarbon (HCFC) replacements for the chlorofluorocarbon (CFC) refrigerants, in water and air. TFA is derivatized to the volatile methyl trifluoroacetate (MTFA) and determined by automated headspace gas chromatography (HSGC) with electron-capture detection or manual HSGC using GC/MS in the selected ion monitoring (SIM) mode. The method is based on the reaction of an aqueous sample containing TFA with dimethyl sulfate (DMS) in concentrated sulfuric acid in a sealed headspace vial under conditions favoring distribution of MTFA to the vapor phase. Water samples are prepared by evaporative concentration, during which TFA is retained as the anion, followed by extraction with diethyl ether of the acidified sample and then back-extraction of TFA (as the anion) in aqueous bicarbonate solution. The extraction step is required for samples with a relatively high background of other salts and organic materials. Air samples are collected in sodium bicarbonate-glycerin-coated glass denuder tubes and prepared by rinsing the denuder contents with water to form an aqueous sample for derivatization and analysis. Recoveries of TFA from spiked water, with and without evaporative concentration, and from spiked air were quantitative, with estimated detection limits of 10 ng/mL (unconcentrated) and 25 pg/mL (concentrated 250 mL:1 mL) for water and 1 ng/m(3) (72 h at 5 L/min) for air. Several environmental air, fogwater, rainwater, and surface water samples were successfully analyzed; many showed the presence of TFA.

show abstract

Section: Virusesmentioning

confidence: 99%

An Analytical Method for Trifluoroacetic Acid in Water and Air Samples Using Headspace Gas Chromatographic Determination of the Methyl Ester

Zehavi

Seiber

1996

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…No similar effect has been reported in the literature, except for mention of a higher bacteriophage titer achieved within an emulsion . Bacteriophages may be protected against inactivation due to “charge shielding” between bacteriophage surface proteins and nano‐emulsion droplets . This shielding mechanism, which could preserve lytic activity, and combined with more favorable contact between bacteriophage and bacteria in the presence of emulsion, could result in an enhanced killing effect.…”

Section: Resultsmentioning

confidence: 89%

“…49 Bacteriophages may be protected against inactivation due to "charge shielding" between bacteriophage surface proteins and nano-emulsion droplets. 50 This shielding mechanism, which could preserve lytic activity, and combined with more favorable contact between bacteriophage and bacteria in the presence of emulsion, could result in an enhanced killing effect. The literature suggests that certain surfactants (e.g., emulsan) do not interfere with phage binding to bacterial surfaces; moreover, the binding occurs at the emulsion interface.…”

Section: Efficacy Of Bacteriophage Preparationmentioning

confidence: 99%

Enhancement of the antimicrobial properties of bacteriophage‐K via stabilization using oil‐in‐water nano‐emulsions

Esteban

Alves

Enright

et al. 2014

Biotechnology Progress

View full text Add to dashboard Cite

Bacteriophage therapy is a promising new treatment that may help overcome the threat posed by antibiotic-resistant pathogenic bacteria, which are increasingly identified in hospitalized patients. The development of biocompatible and sustainable vehicles for incorporation of viable bacterial viruses into a wound dressing is a promising alternative. This article evaluates the antimicrobial efficacy of Bacteriophage K against Staphylococcus aureus over time, when stabilized and delivered via an oil-in-water nano-emulsion. Nano-emulsions were formulated via thermal phase inversion emulsification, and then bacterial growth was challenged with either native emulsion, or emulsion combined with Bacteriophage K. Bacteriophage infectivity, and the influence of storage time of the preparation, were assessed by turbidity measurements of bacterial samples. Newly prepared Bacteriophage K/nano-emulsion formulations have greater antimicrobial activity than freely suspended bacteriophage. The phage-loaded emulsions caused rapid and complete bacterial death of three different strains of S. aureus. The same effect was observed for preparations that were either stored at room temperature (18-20°C), or chilled at 4°C, for up to 10 days of storage. A response surface design of experiments was used to gain insight on the relative effects of the emulsion formulation on bacterial growth and phage lytic activity. More diluted emulsions had a less significant effect on bacterial growth, and diluted bacteriophage-emulsion preparations yielded greater antibacterial activity. The enhancement of bacteriophage activity when delivered via nano-emulsions is yet to be reported. This prompts further investigation into the use of these formulations for the development of novel anti-microbial wound management strategies.

show abstract

“…Nonionic surfactants Triton X-100 and Tween 80 and ionic surfactant SDS are commonly used in virus adsorption/elution to control the surface tension of the fluid and to prevent virus adsorption. While Triton X-100 would be expected to affect nonionic adsorption only, the anionic SDS would be expected to affect both ionic and nonionic adsorption and specifically counteract the adsorption forces (American Society for Testing and Materials, 1995;Fujito, B.T., Lytle, C.D. 1996;Lytle et al, 1992;Retta et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Concentration and detection of hepatitis A virus and its indicator from artificial seawater using zeolite

Cormier

Janes

2016

Journal of Virological Methods

View full text Add to dashboard Cite

Hepatitis A virus (HAV) infection is the leading worldwide cause of acute viral hepatitis, and outbreaks caused by this virus often occur in fecal polluted waters. Rapid concentration and detection of viral contamination in water environments can prevent economic loss and can identify the source of contamination within a short time. However, conventional methods for virus concentration are often laborious, time consuming, and subject to clogging. Furthermore, most methods require a secondary concentration step to reduce the final volume of samples. We developed a method to concentrate HAV from seawater using zeolite in aid of rapid detection. In this method,artificial seawater was inoculated with HAV (7-8 log TCID50) and filtered with zeolite. The viruses were then eluted from zeolite with sodium dodecyl sulfate and detected via real-time PCR (qPCR). Zeolite was able to concentrate HAV from artificial seawater with ∼99% efficiency in less than 5min and was more efficient in seawater than in fresh water. The entire concentration and detection can be done in approximately 2h. Compared to existing methods, this method eliminated the need for a secondary concentration step as well as the necessity to modify the pH or salinity of the seawater during concentration, and was simple and inexpensive.

show abstract

Elution of viruses by ionic and nonionic surfactants

Cited by 22 publications

References 11 publications

An Analytical Method for Trifluoroacetic Acid in Water and Air Samples Using Headspace Gas Chromatographic Determination of the Methyl Ester

An Analytical Method for Trifluoroacetic Acid in Water and Air Samples Using Headspace Gas Chromatographic Determination of the Methyl Ester

Enhancement of the antimicrobial properties of bacteriophage‐K via stabilization using oil‐in‐water nano‐emulsions

Concentration and detection of hepatitis A virus and its indicator from artificial seawater using zeolite

Contact Info

Product

Resources

About