Elucidating the mechanism of miRNA-214 in the regulation of gingival carcinoma

Abstract

Abstract. The aim of the present study was to evaluate the expression levels of microRNA (miRNA)-214 in tumor tissue, blood and saliva of patients with gingival carcinoma, and to investigate the mechanisms underlying the infiltration and invasion of gingival carcinoma. Between January 2013 and March 2015, blood and saliva samples, gingival carcinoma tumor specimens and peritumoral tumor tissues were harvested from 56 patients with gingival carcinoma. Blood and saliva samples were also harvested from 33 control patients without gingival carcinoma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect miRNA-214 and protein tyrosine phosphatase gene (PTEN) mRNA levels. Western blotting and ELISA were performed to detect PTEN protein levels. The results of RT-qPCR demonstrated that the expression of PTEN mRNA in tumor tissues, blood and saliva of patients with gingival carcinoma were significantly decreased compared with that of the control group (P<0.05). These findings were consistent were consistent with the results of PTEN protein expression detected via western blotting and ELISA in these samples (P<0.05). Conversely, the expression levels of miRNA-214 in these samples were significantly increased (P<0.05) in patients with gingival carcinoma compared with the control group. The decreased expression of PTEN may be associated with the expression of miRNA-214. miRNA-214 may regulate infiltration and invasion of gingival carcinoma via PTEN. These results suggest that miRNA-214 may be used as a marker of gingival carcinoma.

<p>Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma</p>

“…Given the critical roles of miR-214 in OSCC, 13,14,34 we investigated the biological function of the miR-214 inhibitor in Cal27 cells delivered via GO-PEI complexes. Transwell assays and wound healing assays were performed to investigate the effect of GO-PEI-inhibitor cell metastasis and migration.…”

“…[9][10][11] Among a variety of miRNAs, miR-214 has been shown to promote tumor progression by regulating multiple signal pathways in OSCC. [12][13][14] It has been reported that miR-214 knockdown inhibits tongue squamous cell carcinoma proliferation and promotes cell apoptosis. 15 Therefore, miRNA-214 could serve as a potential therapeutic target in OSCC.…”

The natural product lapiferin inhibits cell proliferation and promotes cell apoptosis in gingival squamous cell carcinoma via P21 regulation

“…The majority of gingival tumors are highly differentiated squamous cell carcinomas, including gingival squamous cell carcinoma (GSCC) ( 1 ). GSCC occurs in either the maxilla or the mandible ( 2 , 3 ).…”

“…In addition, GSCC is associated with a high risk of metastasis and bone invasion with delayed diagnosis ( 5 , 6 ). In the early stages, GSCC is able to infiltrate into mandibular alveoli, leading to bone destruction, tooth mobility and pain ( 1 ). Chemotherapy is the main therapeutic strategy used to treat malignancies; for example, cisplatin, a DNA-damaging agent, has potent antitumor properties and is commonly used to treat various cancer types, including lung cancer ( 7 ), ovarian cancer ( 8 ), and head and neck carcinomas, particularly oral squamous cell carcinoma ( 9 ).…”

MicroRNA-214 Suppresses Ovarian Cancer by Targeting β-Catenin

“…Therefore, the miRNA expression can indicate the prognosis of human cancers [14-18]. MicroRNA-214 (miR-214) has been found to be aberrantly expressed in different types of human tumors with varied roles in the development of cancers [19-24]. For instance, the expression of miR-214 was up-regulated in melanoma and promoted cancer cell progression by targeting TFAP2C and upregulating ALCAM [25, 26].…”