MicroRNA-214 Suppresses Ovarian Cancer by Targeting β-Catenin

Abstract

Background/Aims: Ovarian cancer is one of the most common malignancies with a high rate of mortality in women. However, current therapies for ovarian cancer treatment are ineffective. Therefore, novel target identification is an urgent requisite. The present study aimed to investigate the role of microRNA-214 (miR-214) in ovarian cancer. Methods: The expression of miR-214, β-catenin, cyclin D1, c-myc, and TCF-1 at the transcriptional level was measured by real-time PCR, while that of β-catenin, Cyclin D1, and c-Myc at the protein level were detected by western blot. Colony formation assay and transwell assay were used to explore the invasion ability of the cancer cells. Cell cycle was measured by flow cytometry. Results: Real-time PCR showed that miR-214 expression in ovarian cancer cell lines was lower than that in the human normal ovarian epithelial cells, IOSE80. Furthermore, the low expression of miR-214 was correlated with high pathological grade. The rate of colony formation and invasion of miR-214 overexpression in SKOV-3 cells were weaker than that in control cells. Moreover, miR-214 overexpression led to the G0/G1 phase arrest. The expression of β-catenin, Cyclin D1, and c-Myc was suppressed by the overexpression of miR-214. Conclusion: These results suggested that miR-214 may serve as a tumor suppressor of ovarian cancer by targeting the β-catenin pathway.

Inhibition of miR‑214 attenuates the migration and invasion of triple‑negative breast cancer cells

“…Increasing evidence has demonstrated that miR-214 is aberrantly expressed and its function is altered in various types of tumor; in particular, low miR-214 expression has been detected in liver cancer and ovarian carcinoma (21,33), whereas high miR-214 expression has been observed in BC, gastric cancer and pancreatic cancer (20,24,34). Notably, Kalniete et al (20) detected high expression levels of miR-214 in patients with TNBC.…”

“…The abnormal expression of miR-214 contributes to the formation of various human tumors, including ovarian cancer, colorectal cancer, gastric cancer and BC (19–22). Furthermore, miR-214 is closely associated with regulation of the development of tumor cells, including cell growth, apoptosis and metastasis (21,23–25); however, at present, the role and target of miR-214 in TNBC is not fully understood.…”

Wnt Signaling in Ovarian Cancer Stemness, EMT, and Therapy Resistance

“…Several other miRs have been demonstrated to impact migration, invasion, and cancer progression via Wnt signaling in ovarian cancer [47,48,49,50,51,52,53,54,55,56,57,58,59]. Interestingly, miR-939 has been suggested to function as a tumor promotor by regulating Wnt signaling through direct suppression of the previously discussed APC2 tumor suppressor [48].…”

Roles of microRNAs in Ovarian Cancer Tumorigenesis: Two Decades Later, What Have We Learned?

“… Cell cycle progression and major regulatory miRNAs in ovarian cancer. This figure was drawn based on the information from Liu et al ( 183 ), Shi et al ( 190 ), Qie et al ( 221 ), and Liu et al ( 224 ). …”